DAFT-seq is short for "Directional Amplification-free Transcriptome sequencing". It's a PCR-free RNA-seq protocol that was
Chappell et al 2019: https://www.biorxiv.org/content/10.1101/852038v2
Siegel*, Chappell* et al. 2020: https://www.biorxiv.org/content/10.1101/2020.03.20.998294v1
With a bit of luck this software should be able to reproduce the analysis of the DAFT-seq data in the manuscripts linked above
- tophat version 2
- samtools Version: 0.1.19-44428
- bedtools v2.17.0