#!/usr/bin/env python
# encoding: utf-8
"""
example2.py
Created by Hariharan Jayaram on 2009-01-28.
Copyright (c) 2009 __MyCompanyName__. All rights reserved.
"""
import masterplate
import plate
import component
def main():
# Create the plate in this case each well will have 2000 ml
mp = masterplate.Masterplate(2000)
p = plate.Plate("A1","H12",mp)
# Define a component with concentration units that you keep constant for this component. i.e
# if you use percent then you remember to use percent everytime you want to dispense this component
c = component.Component("CaAc2",2000,100000)
# Gradient along X dispensed a gradient of component along x ( i.e along number axis on 96 well plate)
p.gradient_along_x(c,0,200)
peg400 = component.Component("peg400",50,100000)
# Gradient along Y dispensed a gradient of component along y ( i.e along alphabet axis)
p.gradient_along_y(peg400,22,30)
# Now we will use a Tribuffer as described by Newman J et al
t1 = component.Component("tribfferpH4",1.0,100000)
t2 = component.Component("tribufferpH10",1.0,100000)
# Say we want to do a pH from 4 to 10 along the x axis we do
p.maketo100_alongx(t1,t2,0.1,0,100)
Water = component.Component("100.00% Water",100,100000)
p.fill_water(Water)
# The makefileforformulatrix method of masterplate.Masterplate Class writes the dispense list
mp.makefileforformulatrix("example6_newman.dl.txt")
if __name__ == '__main__':
main()
