#!/usr/bin/env python
# encoding: utf-8
"""
example2.py
Created by Hariharan Jayaram on 2009-01-28.
Copyright (c) 2009 __MyCompanyName__. All rights reserved.
"""
import masterplate
import plate
import component
def main():
# Create the plate in this case each well will have 2000 ml
mp = masterplate.Masterplate(2000)
p = plate.Plate("A1","H12",mp)
# Define a component with concentration units that you keep constant for this component. i.e
# if you use percent then you remember to use percent everytime you want to dispense this component
nabr = component.Component("NaBr1M",1000,100000)
peg300 = component.Component("peg300",60,300000)
acid = component.Component("newmanpH4",1.0,100000)
base = component.Component("newmanph10",1.0,100000)
# Gradient along X dispensed a gradient of component along x ( i.e along number axis on 96 well plate)
p.gradient_along_y(peg300,35,43)
p.constant_salt(nabr,100)
# Now we will use a Tribuffer as described by Newman J et al
# Say we want to do a pH from 4 to 10 along the x axis we do
p.maketo100_alongx(acid,base,0.1,100,0)
Water = component.Component("100.00% Water",100,100000)
p.fill_water(Water)
# The makefileforformulatrix method of masterplate.Masterplate Class writes the dispense list
mp.makefileforformulatrix("ericNabr100_newman.dl.txt")
if __name__ == '__main__':
main()
