PhyloProfile is an R package that comes together with a Shiny-based tool for integrating, visualizing and exploring multi-layered phylogenetic profiles.
Alongside the presence/absence patterns of orthologs across large taxon collections, PhyloProfile allows the integration of any two additional information layers. These complementary data, like sequence similarity between orthologs, similarities in their domain architecture, or differences in functional annotations enable a more informed interpretation of phylogenetic profiles.
By utilizing the NCBI taxonomy, PhyloProfile can dynamically collapse taxa into higher systematic groups. This enables rapidly changing the resolution from the comparative analyses of proteins in individual species to that of entire kingdoms or even domains without changes to the input data.
PhyloProfile furthermore allows for a dynamic filtering of profiles – taking the taxonomic distribution and the additional information layers into account. This, along with functions to estimate the age of genes and core gene sets facilitates the exploration and analysis of large phylogenetic profiles.
Table of Contents
Installation & Usage
PhyloProfile requires R (version ≥ 3.6.0). Please install or update R on your computer before continue.
Then start R to install and use PhyloProfile.
PhyloProfile is available at Bioconductor (require Bioc version ≥ 3.10). To install PhyloProfile, start R (version ≥ 3.6.0) and enter:
if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager") BiocManager::install("PhyloProfile")
To install the development version of PhyloProfile, please use the devel version of Bioconductor:
if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager") BiocManager::install(version='devel') BiocManager::install("PhyloProfile")
or install the dev version of PhyloProfile from our github repository using devtools.
The dev version of PhyloProfile can be installed from this github repository using
if (!requireNamespace("devtools")) install.packages("devtools") devtools::install_github("BIONF/PhyloProfile", INSTALL_opts = c('--no-lock'), build_vignettes = TRUE)
This step can take a while, as the tool will try do download and install all necessary dependencies automatically. (Note: Depending on your system this sometimes fails, please check the console log for error messages concerning the dependency installation)
Start PhyloProfile's Shiny app
From the R terminal, enter:
Check your web browser, PhyloProfile will be displayed there ;-) For the first time running, the tool will download a pre-caculated taxonomy data. Please be patient until you see a message for uploading input files.
Please check our detailed instructions if you encounter any problems while installing and starting the program.
PhyloProfile can read a number of different input files, including multi-FASTA files, regular tab-separated files, OMA ID lists or OrthoXML files. The additional information layers can be embedded in the OrthoXML or be provided separately as tab-separated files.
Walkthrough and Examples
Read the walkthrough slides to explore the functionality of the PhyloProfile GUI.
Check the vignette for learning how to use PhyloProfile's functions in some specific use-cases:
We would like to thank
- Bastian for the great initial idea and his kind support,
- Members of Ebersberger group for many valuable suggestions and ...bug reports :)
This tool is released under MIT license.
Ngoc-Vinh Tran, Bastian Greshake Tzovaras, Ingo Ebersberger; PhyloProfile: Dynamic visualization and exploration of multi-layered phylogenetic profiles, Bioinformatics, , bty225, https://doi.org/10.1093/bioinformatics/bty225
or use the citation function in R CMD to have it directly in BibTex or LaTeX format
Vinh Tran firstname.lastname@example.org