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Profile HMM-based hybrid error correction algorithm for long reads
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README.md

README.md

Hercules: a profile HMM-based hybrid error correction algorithm for long reads

Installing Hercules

  • Make sure you have a compiler that has support for C++14.
  • Download the code from its GitHub repository.
git clone https://github.com/BilkentCompGen/hercules.git
  • Change directory to hercules/src/ and run the Makefile. If everything goes well, you will have a binary called hercules inside the bin folder.
cd hercules/src/
make
cd ../bin/

Now you can copy this binary wherever you want (preferably under a directory that is included in your $PATH). Assuming that you are in the directory that the binary exists, you may run the command below to display the help message.

./hercules -h

Running Preprocessing step

To display the help message for the preprocessing step, you may run:

./hercules -1 -h

Assume that you have paired-end short reads and a long read (short_1.fastq, short_2.fastq, long.fasta). Then you may simply run:

mkdir preprocessing
./hercules -1 -li long.fasta -si short_1.fastq -si short_2.fastq -o preprocessing/

Note that the output folder preprocessing must exists prior to run the following command. The output of command above will give you the necessary information to proceed for the next steps until the correction step. You should just simply align compressed short reads to the compressed long reads (i.e. both are located in the output folder preprocessing). You must also sort them and preferably remove the duplicates. If you have bowtie2 installed in one of your $PATH directories, then you may simply run:

../utils/runBowtieRmDup.sh preprocessing/compressed_long.fasta preprocessing/compressed_short.fasta bowtie 30

This will run bowtie2 in 30 threads to align compressed short reads to the compressed long reads. The resulting alignment file will be stored in the directory bowtie with a file name alignment.bam. Note that this file will already be sorted and its duplicates will be removed. You do not need to run afteralignment.sh after runBowtieRmDup.sh. However, if you want to use another aligner without sorting its output file, then you must call afteralignment.sh to sort and remove its duplicates unless you want to do it by yourself:

../utils/afteralignment.sh alignment.bam output_alignment.bam 30 8G

Resulting alignment file will be output_alignment.bam. Note that the command above will use 30 threads and 8G of your memory while sorting.

Correction step

To get information about the parameters for the preprocessing step, you may run:

./hercules -2 -h

Assume that you have your alignment file alignment.bam, original long reads long.fasta, short reads (uncompressed, generated during preprocessing step) preprocessing/short.fasta and you would like to store corrected reads inside corrected_long.fasta. The command below will use 30 threads while correcting the original long reads:

./hercules -2 -li long.fasta -ai alignment.bam -si preprocessing/short.fasta -t 30 -o corrected_long.fasta

Resulting fasta file corrected_long.fasta will be the final output of Hercules.

Running Hercules via Docker

To build a Docker image:

cd docker
docker build . -t hercules:latest

Your image named "hercules" should be ready. You can run hercules using this image by

docker run --user=$UID -v /path/to/inputs:/input -v /path/to/outputdir:/output hercules [args]
  • [args] are usual arguments you would pass to hercules executable. Be careful about mapping. You need to specify folders respective to container directory structure.
  • You need to map host machine input and output directory to responding volume directories inside the container. These options are specified by '-v' argment.
  • Docker works with root user by default. "--user" option saves your outputs.
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