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LOCUS pOpenMTA 2977 bp ds-DNA circular 19-JUL-2017
DEFINITION .
KEYWORDS "creator:Keoni Gandall" "marker:GenR"
FEATURES Location/Qualifiers
promoter 1398..1403
/label="tac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 2217..2220
/label="Methylation site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
rep_origin 2357..2945
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind 1215..1231
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 2946..2949
/label="ori"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
source 26..473
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 1300..1310
/label="BsmBI"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 2221..2231
/label="BsmBI"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 2207..2211
/label="5bp-cut"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
gene 2946..2949
/label="repA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1355..1360
/label="RBS"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS complement(588..1121)
/label="GenR"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
misc_feature complement(1260..1278)
/label="SLiCE-rev"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 1420..1426
/label="tac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
source 2946..2949
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 1469..2185
/label="GFP"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1278..1299
/label="BioBrick prefix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
terminator 2288..2335
/label="T7 terminator"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
RBS 1451..1462
/label="RBS"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1377..1381
/label="5bp-cut"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 1404..1419
/label="tac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(1122..1214)
/label="ApmP"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 2235..2249
/label="BioBrick suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
source 2946..2949
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
gene 26..50
/label="repA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 2336..2339
/label="elim-seq"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer_bind complement(2274..2292)
/label="T7 Reverse"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 26..587
/label="ori"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 1368..1370
/label="ATG"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
promoter 1325..1343
/label="T7 promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
source 26..473
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 1322..1367
/label="T7-RBS"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
terminator 1232..1263
/label="tonB terminator"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
gene complement(426..473)
/label="pBS72_0020"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 2255..2273
/label="SLiCE-for"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer_bind complement(2340..2356)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_binding 277..285
/label="DnaA binding site"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
ORIGIN
1 CTCTCTTAAG GTAGCACCTG CGACCaatcg aactaagaaa agtgcaataa actgctgaag
61 tatttcagca gtttttttta tttagaaata gtgaaaaaaa tataatcagg gaggtatcaa
121 tatttaatga gtactgattt aaatttattt agactggaat taataattaa cacgtagact
181 aattaaaatt taatgaggga taaagaggat acaaaaatat taatttcaat ccctattaaa
241 ttttaacaag ggggggatta aaatttaatt agaggtttat ccacaagaaa agaccctaat
301 aaaattttta ctagggttat aacactgatt aatttcttaa tgggggaggg attaaaattt
361 aatgacaaag aaaacaatct tttaagaaaa gcttttaaaa gataataata aaaagagctt
421 tgcgattaag caaaactctt tactttttca ttgacattat caaattcatc gatatgacac
481 acattaacaa atttcgtgag gagtctccag aagaatgcca ttaaatgaac gaagtagagc
541 gaggacaaaa taatgcgggt atagtggagt atcaggttgt gccataaTTA CGTTGCAGTG
601 GATGGGTCAA TATCGAAGTG CATAACTTCC TCACGAATAC CCAACTTGGT GTATAATGCC
661 ACTGCGGGAT CATCACCATA GTCAGCTTGA ACATAAATTA CATATGCTCC AAGGGCATTT
721 GCCTCATGTT TCAACAGATT GATTAATGCA GTGGCAATGC CCTGGCGGCG GTGTTCCCCC
781 GAAACTGCAA GATCGTAGAT GTAAATTTCT GAACGCGGTT GCTCGAAACG CGGTAAGACA
841 TAGGCGGCCA ATGCTCCTAC AACTGCTTCT TGGTCGAATG CTGCTAAGGC AATAAAGGTC
901 TTGGAGCGCA ATAAGTTGCC TAAATAATCT GAGTCAGGTT GATGTTGGGA ATAGGTAGCG
961 ACATCCCCAA ACTCACGCCC AAATAAATCC AACGCCGCAC GCATACTTTT CACTTGGTCT
1021 GGACCCAAGC GGCACGTACG AATGATCCCC ATGCTTGAAC CCCCAAGTTT GGTTTTCGGA
1081 CGGCTCCCCT GCTGAGTAAC GTCGTTTGAA CTGCGCAGCA Tcagtcgatc atagcacgat
1141 caacggcact gttgcaaata gtcggtggtg ataaacttat catccccttt tgctgatgga
1201 gctgcacatg aaccGTAAAA CGACGGCCAG Tagtcaaaag cctccgaccg gaggcttttg
1261 actTGGTCAG GTGGAGTgaa ttcgcggccg cttctagagt cggcgagacg GCTCTTCGTA
1321 Agaataatac gactcactat agggcttaag tataaggagg aaaaaatATG GGTCTCTAAT
1381 GCGGTCTTCC GCATCGCTTG ACAATTAATC ATCGGCTCGT ATAATGtgtg gaatttcaca
1441 catactagag aaagaggaga aatactagAT GAGTAAGGGA GAGGAATTGT TCACCGGGGT
1501 TGTTCCCATT TTGGTGGAAC TGGATGGTGA TGTTAATGGG CACAAATTTT CCGTGTCGGG
1561 TGAGGGAGAA GGTGACGCAA CATACGGTAA GTTGACTCTG AAATTTATTT GTACAACTGG
1621 GAAACTTCCC GTACCGTGGC CGACATTGGT AACAACTTTC TCATACGGGG TACAATGCTT
1681 CTCACGCTAC CCAGACCACA TGAAGCAACA TGATTTTTTT AAAAGCGCTA TGCCTGAAGG
1741 GTATGTGCAA GAACGCACCA TCTTCTTCAA AGATGACGGA AATTATAAAA CCCGCGCGGA
1801 AGTGAAATTT GAAGGAGACA CCTTAGTTAA TCGTATCGAG CTGAAAGGTA TTGACTTCAA
1861 AGAAGATGGC AACATTTTAG GACATAAACT TGAGTACAAC TATAATAGCC ACAACGTCTA
1921 TATTATGGCC GACAAGCAAA AAAACGGGAT TAAGGTTAAT TTCAAAATCC GCCATAACAT
1981 TGAGGACGGC TCAGTCCAGT TAGCAGATCA TTACCAACAA AACACGCCAA TCGGCGACGG
2041 ACCCGTGTTG CTGCCGGATA ACCACTACCT TTCCACTCAG AGCGCCTTGT CGAAGGACCC
2101 AAACGAGAAA CGTGATCACA TGGTTTTATT GGAATTTGTA ACAGCAGCGG GGATTACGCA
2161 TGGGATGGAC GAACTGTATA AGTAAGCGAT GTTGAAGACC ATGAAGAGCT TAGAGACCGG
2221 cgtctctgac cAAAtactag tTCctgcagG GCTCGAGGTG TCAATCGTCG GaGCCGCTGA
2281 GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG
2341 TCATAGCTGT TTCCTGTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
2401 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
2461 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
2521 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
2581 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
2641 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
2701 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
2761 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
2821 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
2881 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
2941 GGAAAaatcA ATGGCAGGTT AGGGATAACA GGGTAAT
//