diff --git a/CHANGELOG.md b/CHANGELOG.md
index 68397452c..5b32ce1b2 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -7,6 +7,10 @@ This project adheres to [Semantic Versioning](http://semver.org/).
 
 - Adds retroseq for mobile element detection
 
+### Databases
+
+- expansionhunter variant catalog: v4.0.2 -> v5.0.0
+
 ### Tools
 
 - RetroSeq: 9d4f3b5
diff --git a/definitions/rd_dna_parameters.yaml b/definitions/rd_dna_parameters.yaml
index 29eb40ca9..d048e6d2b 100755
--- a/definitions/rd_dna_parameters.yaml
+++ b/definitions/rd_dna_parameters.yaml
@@ -880,7 +880,7 @@ expansionhunter_variant_catalog_file_path:
   associated_recipe:
     - expansionhunter
   data_type: SCALAR
-  default: grch37_expansionhunter_variant_catalog_-4.0.2-.json
+  default: grch37_expansionhunter_variant_catalog_-5.0.0-.json
   exists_check: file
   is_reference: 1
   reference: reference_dir
diff --git a/t/data/references/grch37_expansionhunter_variant_catalog_-4.0.2-.json b/t/data/references/grch37_expansionhunter_variant_catalog_-5.0.0-.json
similarity index 100%
rename from t/data/references/grch37_expansionhunter_variant_catalog_-4.0.2-.json
rename to t/data/references/grch37_expansionhunter_variant_catalog_-5.0.0-.json
diff --git a/templates/mip_download_rd_dna_config_-1.0-.yaml b/templates/mip_download_rd_dna_config_-1.0-.yaml
index e5daeb2e6..585795270 100644
--- a/templates/mip_download_rd_dna_config_-1.0-.yaml
+++ b/templates/mip_download_rd_dna_config_-1.0-.yaml
@@ -41,9 +41,8 @@ reference:
     - 20150915
     - 20200310
   expansionhunter:
-    - 3.0.1
-    - 3.1.2
     - 4.0.2
+    - 5.0.0
   gatk_mitochondrial_ref:
     - v0_m_amb
     - v0_m_ann
@@ -445,20 +444,6 @@ reference_feature:
         url_prefix: https://raw.githubusercontent.com/dellytools/delly/master/excludeTemplates/
   expansionhunter:
     grch37:
-      3.0.1:
-        file: variant_catalog_grch37.json
-        file_check: variant_catalog_grch37.json.md5
-        outfile: grch37_expansionhunter_variant_catalog_-3.0.1-.json
-        outfile_check: grch37_expansionhunter_variant_catalog_-3.0.1-.json.md5
-        outfile_check_method: md5sum
-        url_prefix: https://raw.githubusercontent.com/Clinical-Genomics/reference-files/master/rare-disease/disease_loci/ExpansionHunter-v3.0.1/
-      3.1.2:
-        file: variant_catalog_grch37.json
-        file_check: variant_catalog_grch37.json.md5
-        outfile: grch37_expansionhunter_variant_catalog_-3.1.2-.json
-        outfile_check: grch37_expansionhunter_variant_catalog_-3.1.2-.json.md5
-        outfile_check_method: md5sum
-        url_prefix: https://raw.githubusercontent.com/Clinical-Genomics/reference-files/master/rare-disease/disease_loci/ExpansionHunter-v3.1.2/
       4.0.2:
         file: variant_catalog_grch37.json
         file_check: variant_catalog_grch37.json.md5
@@ -466,21 +451,14 @@ reference_feature:
         outfile_check: grch37_expansionhunter_variant_catalog_-4.0.2-.json.md5
         outfile_check_method: md5sum
         url_prefix: https://raw.githubusercontent.com/Clinical-Genomics/reference-files/master/rare-disease/disease_loci/ExpansionHunter-v4.0.2/
-    grch38:
-      3.0.1:
-        file: variant_catalog_grch38.json
-        file_check: variant_catalog_grch38.json.md5
-        outfile: grch38_expansionhunter_variant_catalog_-3.0.1-.json
-        outfile_check: grch38_expansionhunter_variant_catalog_-3.0.1-.json.md5
-        outfile_check_method: md5sum
-        url_prefix: https://raw.githubusercontent.com/Clinical-Genomics/reference-files/master/rare-disease/disease_loci/ExpansionHunter-v3.0.1/
-      3.1.2:
-        file: variant_catalog_hg38.json
-        file_check: variant_catalog_hg38.json.md5
-        outfile: grch38_expansionhunter_variant_catalog_-3.1.2-.json
-        outfile_check: grch38_expansionhunter_variant_catalog_-3.1.2-.json.md5
+      5.0.0:
+        file: variant_catalog_grch37.json
+        file_check: variant_catalog_grch37.json.md5
+        outfile: grch37_expansionhunter_variant_catalog_-5.0.0-.json
+        outfile_check: grch37_expansionhunter_variant_catalog_-5.0.0-.json.md5
         outfile_check_method: md5sum
-        url_prefix: https://raw.githubusercontent.com/Clinical-Genomics/reference-files/master/rare-disease/disease_loci/ExpansionHunter-v3.1.2/
+        url_prefix: https://raw.githubusercontent.com/Clinical-Genomics/reference-files/master/rare-disease/disease_loci/ExpansionHunter-v5.0.0/
+    grch38:
       4.0.2:
         file: variant_catalog_hg38.json
         file_check: variant_catalog_hg38.json.md5
@@ -488,6 +466,13 @@ reference_feature:
         outfile_check: grch38_expansionhunter_variant_catalog_-4.0.2-.json.md5
         outfile_check_method: md5sum
         url_prefix: https://raw.githubusercontent.com/Clinical-Genomics/reference-files/master/rare-disease/disease_loci/ExpansionHunter-v4.0.2/
+      5.0.0:
+        file: variant_catalog_hg38.json
+        file_check: variant_catalog_hg38.json.md5
+        outfile: grch38_expansionhunter_variant_catalog_-5.0.0-.json
+        outfile_check: grch38_expansionhunter_variant_catalog_-5.0.0-.json.md5
+        outfile_check_method: md5sum
+        url_prefix: https://raw.githubusercontent.com/Clinical-Genomics/reference-files/master/rare-disease/disease_loci/ExpansionHunter-v5.0.0/
   gatk_mitochondrial_ref:
     grch38:
       v0_m_amb: