Quick start of NanoDJ VICE app in DE
NanoDJ is a Dockerized Jupyter Notebook for interactive Oxford Nanopore MinION sequence manipulation and genome assembly. It integrates basecalling, read trimming and quality control, simulation and plotting routines with a variety of widely used aligners and assemblers, including procedures for hybrid assembly.
- Facilitate ONT sequence analyses by integrating capabilities for data manipulation, sequence comparison and assembly in field experiments or for educational purposes.
- Interactively explore your data with beautiful visualizations that provide new perspectives.
- Easily share results with your team, even those members without NanoDJ installed.
In this quick start, we will show you how to launch NanoDJ VICE app in DE.
Downloads, access, and services
In order to complete this tutorial you will need access to the following services/software
Prerequisite Preparation/Notes Link/Download CyVerse account You will need a CyVerse account to complete this exercise Register
We will use the following CyVerse platform(s):
|Platform||Interface||Link||Platform Documentation||Learning Center Docs|
|Discovery Environment||Web/Point-and-click||Discovery Environment||DE Manual||Guide|
Input and example data
In order to complete this quickstart you will need to have the following inputs prepared.
The example input data (which contains data and Jupyter notebooks) is already prefilled for you during app launch
|Input File(s)||Format||Preparation/Notes||Example Data|
|Fast5 and Jupyter notebooks||
||Input data can be a list of FAST5 files from previous basecalled runs (e.g. a Metrichor output) or event level signal data to be basecalled using the latest ONT caller||
Get started: Launch NanoDJ
- Login to the Discovery Environment.
- Click on Apps window in the DE workspace and search for and run NanoDJ.
- Under “Analysis Name” leave the defaults or make any desired notes.
- Under “Parameters” for ‘Input files', click the "+" and browse
data_transfer.sh(iplantcollaborative > example_data > nanodj and select
- Click Launch Analysis. You will receive couple of notifications on the bell corresponding to job submission and running with the "Access your running analysis here".
- Clicking on the "Access your running analysis" will open the NanoDJ JupyterLab in another tab in the browser after a brief building phase.
You will be asked to authenticate again to the JupyterLab with your CyVerse username and password
- Open the Terminal in the main JupyterLab interface (under 'others' section), navigate to the
vicefolder and execute the
data_transfer.shscript to initiate the test data downloading process. You will be asked to enter your CyVerse password again to initiate the downloading process.
cd vice bash data_transfer.sh Enter your current iRODS password:
- Finally, once you finish analysis, navigate to the Discovery Environment tab, select the Analysis window and select the analysis, click "save and complete analysis". Upon clicking complete analysis, the analysis will be completed and all the outputs will be brought back to the analysis folder. Alternatively you can use
iput -rPVT <folder name>command to transfer the data back to the data store.
Currently quick starts uses a reduced test data but if you want to try the full test data, then instead of using
data_transfer_full.sh script which is in the same folder as the other script.
Additional information, help
- Full materials for the webinar is available here
- See the original JupyterLab quick start
- See the original NanoDJ for how to run ONT data analysis
- Search for an answer: CyVerse Learning Center or CyVerse Wiki
- Contact CyVerse support by clicking the intercom button on the page.
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