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Quick start of NanoDJ VICE app in DE

Goal

NanoDJ is a Dockerized Jupyter Notebook for interactive Oxford Nanopore MinION sequence manipulation and genome assembly. It integrates basecalling, read trimming and quality control, simulation and plotting routines with a variety of widely used aligners and assemblers, including procedures for hybrid assembly.

  • Facilitate ONT sequence analyses by integrating capabilities for data manipulation, sequence comparison and assembly in field experiments or for educational purposes.
  • Interactively explore your data with beautiful visualizations that provide new perspectives.
  • Easily share results with your team, even those members without NanoDJ installed.

In this quick start, we will show you how to launch NanoDJ VICE app in DE.


Prerequisites

Downloads, access, and services

In order to complete this tutorial you will need access to the following services/software

Prerequisite Preparation/Notes Link/Download
CyVerse account You will need a CyVerse account to complete this exercise Register

Platform(s)

We will use the following CyVerse platform(s):

Platform Interface Link Platform Documentation Learning Center Docs
Discovery Environment Web/Point-and-click Discovery Environment DE Manual Guide

Input and example data

In order to complete this quickstart you will need to have the following inputs prepared.

Note

The example input data (which contains data and Jupyter notebooks) is already prefilled for you during app launch

Input File(s) Format Preparation/Notes Example Data
Fast5 and Jupyter notebooks
  • Fast5
  • Jupyter notebooks
Input data can be a list of FAST5 files from previous basecalled runs (e.g. a Metrichor output) or event level signal data to be basecalled using the latest ONT caller data_transfer.sh (Community Data -> iplantcollaborative -> example_data -> nanodj and select data_transfer.sh script)

Get started: Launch NanoDJ

  1. Login to the Discovery Environment.
  2. Click on Apps window in the DE workspace and search for and run NanoDJ.

Tip

Alternatively you can clik this nanodj logo button to directly launch the NanoDJ VICE app.

  1. Under “Analysis Name” leave the defaults or make any desired notes.
  2. Under “Parameters” for ‘Input files', click the "+" and browse data_transfer.sh (iplantcollaborative > example_data > nanodj and select data_transfer.sh script).
  3. Click Launch Analysis. You will receive couple of notifications on the bell corresponding to job submission and running with the "Access your running analysis here".
  4. Clicking on the "Access your running analysis" will open the NanoDJ JupyterLab in another tab in the browser after a brief building phase.

Note

You will be asked to authenticate again to the JupyterLab with your CyVerse username and password

  1. Open the Terminal in the main JupyterLab interface (under 'others' section), navigate to the vice folder and execute the data_transfer.sh script to initiate the test data downloading process. You will be asked to enter your CyVerse password again to initiate the downloading process.
cd vice
bash data_transfer.sh
Enter your current iRODS password:
  1. Finally, once you finish analysis, navigate to the Discovery Environment tab, select the Analysis window and select the analysis, click "save and complete analysis". Upon clicking complete analysis, the analysis will be completed and all the outputs will be brought back to the analysis folder. Alternatively you can use iput -rPVT <folder name> command to transfer the data back to the data store.

Note

Currently quick starts uses a reduced test data but if you want to try the full test data, then instead of using data_transfer.sh, use data_transfer_full.sh script which is in the same folder as the other script.


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