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DEST

Scripts for mapping and quality control of DEST dataset

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populationInfo\: Has supplemental data from the DrosEU, DrosRTEC, and DPGP files to make a unified meta-datafile. This datafile is supplemental table 1 of Kapun et al 2021. The meta-data file includes collection information, library quality filtering, inversion frequencies, weather stations and WorldClim environmental data.

PoolSeq mapping pipeline

mappingPipeline\: Contains dockerized mapping pipeline. Downloads data, produces bam files, filter files, gSYNC files

Incorporate Drosophila Genome Nexus data

add_DGN_datda\: Downloads, formats, lifts-over the Drosophila Genome Nexus data into gSYNC format

SNP calling

snpCalling\: SNP calling based on gSYNC files. Runs PoolSNP and SNAPE using snakemake pipeline

Example Scripts

examples: Provides a basic script to pull allele frequencies from a GDS table and also calculates the effective coverage

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