diff --git a/docs/cmd_list.txt b/docs/cmd_list.txt
index 94bd326b..0ae11fc0 100644
--- a/docs/cmd_list.txt
+++ b/docs/cmd_list.txt
@@ -31,7 +31,8 @@ Workflow execution commands:
   validate  Validate workflow specification file.
 
 Workflow sharing commands:
-  share-add  Share a workflow with other users (read-only).
+  share-add     Share a workflow with other users (read-only).
+  share-remove  Unshare a workflow.
 
 Workspace interactive commands:
   close  Close an interactive session.
diff --git a/reana_client/api/client.py b/reana_client/api/client.py
index d06aba2a..11e4aa9d 100644
--- a/reana_client/api/client.py
+++ b/reana_client/api/client.py
@@ -1327,3 +1327,41 @@ def share_workflow(
             f"Message: {e.response.json()['message']}"
         )
         raise Exception(e.response.json()["message"])
+
+
+def unshare_workflow(workflow, user_email_to_unshare_with, access_token):
+    """Unshare a workflow with a user.
+
+    :param workflow: name or id of the workflow.
+    :param user_email_to_unshare_with: user to unshare the workflow with.
+    :param access_token: access token of the current user.
+
+    :return: a dictionary containing the ``workflow_id``, ``workflow_name``, and
+             a ``message`` key with the result of the operation.
+    """
+    try:
+        unshare_params = {
+            "workflow_id_or_name": workflow,
+            "user_email_to_unshare_with": user_email_to_unshare_with,
+            "access_token": access_token,
+        }
+
+        (response, http_response) = current_rs_api_client.api.unshare_workflow(
+            **unshare_params
+        ).result()
+
+        if http_response.status_code == 200:
+            return response
+        else:
+            raise Exception(
+                "Expected status code 200 but replied with "
+                f"{http_response.status_code}"
+            )
+
+    except HTTPError as e:
+        logging.debug(
+            "Workflow could not be unshared: "
+            f"\nStatus: {e.response.status_code}\nReason: {e.response.reason}\n"
+            f"Message: {e.response.json()['message']}"
+        )
+        raise Exception(e.response.json()["message"])
diff --git a/reana_client/cli/workflow.py b/reana_client/cli/workflow.py
index cee22892..a1dfb941 100644
--- a/reana_client/cli/workflow.py
+++ b/reana_client/cli/workflow.py
@@ -1545,3 +1545,64 @@ def share_workflow_add(
 
     else:
         display_message(f"Cannot find workflow {workflow}", msg_type="error")
+
+
+@workflow_sharing_group.command("share-remove")
+@check_connection
+@add_workflow_option
+@add_access_token_options
+@click.option(
+    "-u",
+    "--user",
+    "users",
+    multiple=True,
+    help="Users to unshare the workflow with.",
+    required=True,
+)
+@click.pass_context
+def share_workflow_remove(ctx, workflow, access_token, users):  # noqa D412
+    """Unshare a workflow.
+
+    The `share-remove` command allows for unsharing a workflow. The workflow
+    will no longer be visible to the users with whom it was shared.
+
+    Example:
+
+    $ reana-client share-remove -w myanalysis.42 --user bob@example.org
+    """
+    from reana_client.api.client import unshare_workflow
+
+    unshare_errors = []
+    unshared_users = []
+
+    if workflow:
+        try:
+            for user in users:
+                try:
+                    logging.info(f"Unsharing workflow {workflow} with user {user}")
+                    unshare_workflow(workflow, user, access_token)
+                    unshared_users.append(user)
+                except Exception as e:
+                    unshare_errors.append(
+                        f"Failed to unshare {workflow} with {user}: {str(e)}"
+                    )
+                    logging.debug(traceback.format_exc())
+        except Exception as e:
+            logging.debug(traceback.format_exc())
+            logging.debug(str(e))
+            display_message(
+                "An error occurred while unsharing workflow:\n{}".format(str(e)),
+                msg_type="error",
+            )
+
+        if unshared_users:
+            display_message(
+                f"{workflow} is no longer shared with {', '.join(unshared_users)}",
+                msg_type="success",
+            )
+        if unshare_errors:
+            for error in unshare_errors:
+                display_message(error, msg_type="error")
+
+    else:
+        display_message(f"Cannot find workflow {workflow}", msg_type="error")
diff --git a/tests/test_cli_workflows.py b/tests/test_cli_workflows.py
index 4d3c3050..c4af475e 100644
--- a/tests/test_cli_workflows.py
+++ b/tests/test_cli_workflows.py
@@ -1026,3 +1026,38 @@ def test_share_add_workflow():
             )
             assert result.exit_code == 0
             assert response["message"] in result.output
+
+
+def test_share_remove_workflow():
+    """Test share-remove workflows."""
+    status_code = 200
+    response = {
+        "message": "is no longer shared with",
+        "workflow_id": "string",
+        "workflow_name": "string",
+    }
+    env = {"REANA_SERVER_URL": "localhost"}
+    mock_http_response, mock_response = Mock(), Mock()
+    mock_http_response.status_code = status_code
+    mock_response = response
+    reana_token = "000000"
+    runner = CliRunner(env=env)
+    with runner.isolation():
+        with patch(
+            "reana_client.api.client.current_rs_api_client",
+            make_mock_api_client("reana-server")(mock_response, mock_http_response),
+        ):
+            result = runner.invoke(
+                cli,
+                [
+                    "share-remove",
+                    "-t",
+                    reana_token,
+                    "--workflow",
+                    "test-workflow.1",
+                    "--user",
+                    "bob@.cern.ch",
+                ],
+            )
+            assert result.exit_code == 0
+            assert response["message"] in result.output