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My samples had 60-80 million read pairs, however Kraken2 reported 120-160 million fragments (two times as much).
Apparently each read is counted. At the same time same taxa have uneven numbers of associated fragments.
This indicates that the reads of a read pair could be mapped to different taxa.
The text was updated successfully, but these errors were encountered:
Tried to classify samples from paired-end sequencing using the command:
kraken2 --db {database} --paired {query} {mate} --report {report} --threads {threads} > {out} 2> {log}
My samples had 60-80 million read pairs, however Kraken2 reported 120-160 million fragments (two times as much).
Apparently each read is counted. At the same time same taxa have uneven numbers of associated fragments.
This indicates that the reads of a read pair could be mapped to different taxa.
The text was updated successfully, but these errors were encountered: