diff --git a/config.py b/config.py index e6ff35f..6840a7f 100644 --- a/config.py +++ b/config.py @@ -4,14 +4,15 @@ LOG_GROUP_NAME = APP_NAME # DOCKER REGISTRY INFORMATION: -DOCKERHUB_TAG = 'cellprofiler/distributed-cellprofiler:2.0.0_4.1.3' +DOCKERHUB_TAG = 'cellprofiler/distributed-cellprofiler:2.0.0_4.2.4' # AWS GENERAL SETTINGS: AWS_REGION = 'us-east-1' AWS_PROFILE = 'default' # The same profile used by your AWS CLI installation SSH_KEY_NAME = 'your-key-file.pem' # Expected to be in ~/.ssh AWS_BUCKET = 'your-bucket-name' # Bucket to use for logging -SOURCE_BUCKET = 'bucket-name' # Bucket to download files from +SOURCE_BUCKET = 'bucket-name' # Bucket to download image files from +WORKSPACE_BUCKET = 'bucket-name' # Bucket to download non-image files from DESTINATION_BUCKET = 'bucket-name' # Bucket to upload files to UPLOAD_FLAGS = '' # Any flags needed for upload to destination bucket @@ -19,8 +20,8 @@ ECS_CLUSTER = 'default' CLUSTER_MACHINES = 3 TASKS_PER_MACHINE = 1 -MACHINE_TYPE = ['m4.xlarge'] -MACHINE_PRICE = 0.10 +MACHINE_TYPE = ['m5.xlarge'] +MACHINE_PRICE = 0.20 EBS_VOL_SIZE = 30 # In GB. Minimum allowed is 22. DOWNLOAD_FILES = 'False' diff --git a/documentation/DCP-documentation/step_1_configuration.md b/documentation/DCP-documentation/step_1_configuration.md index 6e54969..272cbe7 100644 --- a/documentation/DCP-documentation/step_1_configuration.md +++ b/documentation/DCP-documentation/step_1_configuration.md @@ -25,7 +25,9 @@ For more information and examples, see [External Buckets](external_buckets.md). * **AWS_BUCKET:** The bucket to which you would like to write log files. This is generally the bucket in the account in which you are running compute. -* **SOURCE_BUCKET:** The bucket where the files you will be reading are. +* **SOURCE_BUCKET:** The bucket where the image files you will be reading are. +Often, this is the same as AWS_BUCKET. +* **WORKSPACE:** The bucket where non-image files you will be reading are (e.g. pipeline, load_data.csv, etc.). Often, this is the same as AWS_BUCKET. * **DESTINATION_BUCKET:** The bucket where you want to write your output files. Often, this is the same as AWS_BUCKET. diff --git a/example_project/README.md b/example_project/README.md index 3d064d1..25fdaef 100644 --- a/example_project/README.md +++ b/example_project/README.md @@ -1,3 +1,5 @@ +# Distributed-CellProfiler Minimal Example + Included in this folder is all of the resources for running a complete mini-example of Distributed-Cellprofiler. It includes 3 sample image sets and a CellProfiler pipeline that identifies cells within the images and makes measuremements. It also includes the Distributed-CellProfiler files pre-configured to create a queue of all 3 jobs and spin up a spot fleet of 3 instances, each of which will process a single image set. @@ -9,21 +11,23 @@ It also includes the Distributed-CellProfiler files pre-configured to create a q Before running this mini-example, you will need to set up your AWS infrastructure as described in our [online documentation](https://distributedscience.github.io/Distributed-CellProfiler/step_0_prep.html). This includes creating the fleet file that you will use in Step 3. -Upload the 'sample_project' folder to the top level of your bucket. +Upload the 'sample_project' folder to the top level of your bucket. While in the `Distributed-CellProfiler` folder, use the following command, replacing `yourbucket` with your bucket name: ```bash # Copy example files to S3 BUCKET=yourbucket -aws s3 sync example_project/project_folder s3://${BUCKET}/project_folder +aws s3 sync example_project/demo_project_folder s3://${BUCKET}/demo_project_folder # Replace the default config with the example config cp example_project/config.py config.py ``` ### Step 1 + In config.py you will need to update the following fields specific to your AWS configuration: -``` + +```python # AWS GENERAL SETTINGS: AWS_REGION = 'us-east-1' AWS_PROFILE = 'default' # The same profile used by your AWS CLI installation @@ -32,17 +36,21 @@ AWS_BUCKET = 'your-bucket-name' SOURCE_BUCKET = 'your-bucket-name' # Only differs from AWS_BUCKET with advanced configuration DESTINATION_BUCKET = 'your-bucket-name' # Only differs from AWS_BUCKET with advanced configuration ``` + Then run `python3 run.py setup` ### Step 2 -This command points to the job file created for this demonstartion and should be run as-is. + +This command points to the job file created for this demonstration and should be run as-is. `python3 run.py submitJob example_project/files/exampleJob.json` ### Step 3 + This command should point to whatever fleet file you created in Step 0 so you may need to update the `exampleFleet.json` file name. `python3 run.py startCluster files/exampleFleet.json` ### Step 4 + This command points to the monitor file that is automatically created with your run and should be run as-is. `python3 run.py monitor files/FlyExampleSpotFleetRequestId.json` @@ -51,4 +59,23 @@ This command points to the monitor file that is automatically created with your While the run is happening, you can watch real-time metrics in your Cloudwatch Dashboard by navigating in the [Cloudwatch console](https://console.aws.amazon.com/cloudwatch). Note that the metrics update at intervals that may not be helpful with this fast, minimal example. -After the run is done, you should see your CellProfiler output files in S3 at s3://${BUCKET}/project_folder/output in per-image folders. \ No newline at end of file +After the run is done, you should see your CellProfiler output files in S3 at s3://${BUCKET}/project_folder/output in per-image folders. + +## Cleanup + +The spot fleet, queue, and task definition will be automatically cleaned up after your demo is complete because you are running `monitor`. + +To remove everything else: + +```bash +# Remove files added to S3 bucket +BUCKET=yourbucket +aws s3 rm --recursive s3://${BUCKET}/demo_project_folder + +# Remove Cloudwatch logs +aws logs delete-log-group --log-group-name FlyExample +aws logs delete-log-group --log-group-name FlyExample_perInstance + +# Delete DeadMessages queue +aws sqs delete-queue --queue-url ExampleProject_DeadMessages +``` diff --git 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from example_project/project_folder/images/01_POS076_D.TIF rename to example_project/demo_project_folder/images/01_POS076_D.TIF diff --git a/example_project/project_folder/images/01_POS076_F.TIF b/example_project/demo_project_folder/images/01_POS076_F.TIF similarity index 100% rename from example_project/project_folder/images/01_POS076_F.TIF rename to example_project/demo_project_folder/images/01_POS076_F.TIF diff --git a/example_project/project_folder/images/01_POS076_R.TIF b/example_project/demo_project_folder/images/01_POS076_R.TIF similarity index 100% rename from example_project/project_folder/images/01_POS076_R.TIF rename to example_project/demo_project_folder/images/01_POS076_R.TIF diff --git a/example_project/project_folder/images/01_POS218_D.TIF b/example_project/demo_project_folder/images/01_POS218_D.TIF similarity index 100% rename from example_project/project_folder/images/01_POS218_D.TIF rename to example_project/demo_project_folder/images/01_POS218_D.TIF diff --git a/example_project/project_folder/images/01_POS218_F.TIF b/example_project/demo_project_folder/images/01_POS218_F.TIF similarity index 100% rename from example_project/project_folder/images/01_POS218_F.TIF rename to example_project/demo_project_folder/images/01_POS218_F.TIF diff --git a/example_project/project_folder/images/01_POS218_R.TIF b/example_project/demo_project_folder/images/01_POS218_R.TIF similarity index 100% rename from example_project/project_folder/images/01_POS218_R.TIF rename to example_project/demo_project_folder/images/01_POS218_R.TIF diff --git a/example_project/project_folder/workspace/ExampleFly.cppipe b/example_project/demo_project_folder/workspace/ExampleFly.cppipe similarity index 100% rename from example_project/project_folder/workspace/ExampleFly.cppipe rename to example_project/demo_project_folder/workspace/ExampleFly.cppipe diff --git a/example_project/project_folder/workspace/load_data.csv b/example_project/demo_project_folder/workspace/load_data.csv similarity index 100% rename from example_project/project_folder/workspace/load_data.csv rename to example_project/demo_project_folder/workspace/load_data.csv diff --git a/example_project/files/exampleJob.json b/example_project/files/exampleJob.json index c1ad5ee..e2c1308 100644 --- a/example_project/files/exampleJob.json +++ b/example_project/files/exampleJob.json @@ -1,9 +1,9 @@ { "_comment1": "Paths in this file are relative to the root of your S3 bucket", - "pipeline": "project_folder/workspace/ExampleFly.cppipe", - "data_file": "project_folder/workspace/load_data.csv", - "input": "project_folder/workspace/", - "output": "project_folder/output", + "pipeline": "demo_project_folder/workspace/ExampleFly.cppipe", + "data_file": "demo_project_folder/workspace/load_data.csv", + "input": "demo_project_folder/workspace/", + "output": "demo_project_folder/output", "output_structure": "Metadata_Position", "_comment2": "The following groups are tasks, and each will be run in parallel", "groups": [ diff --git a/example_project_CPG/README.md b/example_project_CPG/README.md new file mode 100644 index 0000000..8fa3506 --- /dev/null +++ b/example_project_CPG/README.md @@ -0,0 +1,82 @@ +# CPG Example Project + +Included in this folder is all of the resources for running a complete mini-example of Distributed-CellProfiler. +This example differs from the other example project in that it reads data hosted in the public data repository the [Cell Painting Gallery](https://github.com/broadinstitute/cellpainting-gallery) instead of reading images from your own bucket. +Workspace files are hosted in your own S3 bucket, and data is output to your bucket, and compute is performed in your account. +It includes the Distributed-CellProfiler files pre-configured to create a queue of 3 jobs and spin up a spot fleet of 3 instances, each of which will process a single image set. + +## Running example project + +### Step 0 + +Before running this mini-example, you will need to set up your AWS infrastructure as described in our [online documentation](https://distributedscience.github.io/Distributed-CellProfiler/step_0_prep.html). +This includes creating the fleet file that you will use in Step 3. + +Upload the 'sample_project' folder to the top level of your bucket. +While in the `Distributed-CellProfiler` folder, use the following command, replacing `yourbucket` with your bucket name: + +```bash +# Copy example files to S3 +BUCKET=yourbucket +aws s3 sync example_project_CPG/demo_project_folder s3://${BUCKET}/demo_project_folder + +# Replace the default config with the example config +cp example_project_CPG/config.py config.py +``` + +### Step 1 + +In config.py you will need to update the following fields specific to your AWS configuration: + +```python +# AWS GENERAL SETTINGS: +AWS_REGION = 'us-east-1' +AWS_PROFILE = 'default' # The same profile used by your AWS CLI installation +SSH_KEY_NAME = 'your-key-file.pem' # Expected to be in ~/.ssh +AWS_BUCKET = 'your-bucket-name' +WORKSPACE_BUCKET = 'your-bucket-name' # Only differs from AWS_BUCKET with advanced configuration +DESTINATION_BUCKET = 'your-bucket-name' # Only differs from AWS_BUCKET with advanced configuration +``` + +Then run `python run.py setup` + +### Step 2 + +This command points to the job file created for this demonstration and should be run as-is. +`python run.py submitJob example_project_CPG/files/exampleCPGJob.json` + +### Step 3 + +This command should point to whatever fleet file you created in Step 0 so you may need to update the `exampleFleet.json` file name. +`python run.py startCluster files/exampleFleet.json` + +### Step 4 + +This command points to the monitor file that is automatically created with your run and should be run as-is. +`python run.py monitor files/ExampleCPGSpotFleetRequestId.json` + +## Results + +While a run is happening, you can watch real-time metrics in your Cloudwatch Dashboard by navigating in the [Cloudwatch console](https://console.aws.amazon.com/cloudwatch). +Note that the metrics update at intervals that may not be helpful with this fast, minimal example. + +After the run is done, you should see your CellProfiler output files in your S3 bucket at s3://${BUCKET}/project_folder/output in per-well-and-site folders. + +## Cleanup + +The spot fleet, queue, and task definition will be automatically cleaned up after your demo is complete because you are running `monitor`. + +To remove everything else: + +```bash +# Remove files added to S3 bucket +BUCKET=yourbucket +aws s3 rm --recursive s3://${BUCKET}/demo_project_folder + +# Remove Cloudwatch logs +aws logs delete-log-group --log-group-name ExampleCPG +aws logs delete-log-group --log-group-name ExampleCPG_perInstance + +# Delete DeadMessages queue +aws sqs delete-queue --queue-url ExampleProject_DeadMessages +``` diff --git a/example_project_CPG/config.py b/example_project_CPG/config.py new file mode 100644 index 0000000..4f77e4c --- /dev/null +++ b/example_project_CPG/config.py @@ -0,0 +1,55 @@ +# Constants (User configurable) + +APP_NAME = 'ExampleCPG' # Used to generate derivative names unique to the application. + +# DOCKER REGISTRY INFORMATION: +DOCKERHUB_TAG = 'erinweisbart/distributed-cellprofiler:workspace_bucket' + +# AWS GENERAL SETTINGS: +AWS_REGION = 'us-east-1' +AWS_PROFILE = 'default' # The same profile used by your AWS CLI installation +SSH_KEY_NAME = 'your-key-file.pem' # Expected to be in ~/.ssh +AWS_BUCKET = 'your-bucket-name' # Bucket to use for logging +SOURCE_BUCKET = 'cellpainting-gallery' # Bucket to download image files from +WORKSPACE_BUCKET = 'your-bucket-name' # Bucket to download non-image files from +DESTINATION_BUCKET = 'your-bucket-name' # Bucket to upload files to + +# EC2 AND ECS INFORMATION: +ECS_CLUSTER = 'default' +CLUSTER_MACHINES = 3 +TASKS_PER_MACHINE = 1 +MACHINE_TYPE = ['c4.xlarge'] +MACHINE_PRICE = 0.13 +EBS_VOL_SIZE = 22 # In GB. Minimum allowed is 22. +DOWNLOAD_FILES = 'True' + +# DOCKER INSTANCE RUNNING ENVIRONMENT: +DOCKER_CORES = 1 # Number of CellProfiler processes to run inside a docker container +CPU_SHARES = DOCKER_CORES * 1024 # ECS computing units assigned to each docker container (1024 units = 1 core) +MEMORY = 7000 # Memory assigned to the docker container in MB +SECONDS_TO_START = 3*60 # Wait before the next CP process is initiated to avoid memory collisions + +# SQS QUEUE INFORMATION: +SQS_QUEUE_NAME = APP_NAME + 'Queue' +SQS_MESSAGE_VISIBILITY = 10*60 # Timeout (secs) for messages in flight (average time to be processed) +SQS_DEAD_LETTER_QUEUE = 'ExampleProject_DeadMessages' + +# LOG GROUP INFORMATION: +LOG_GROUP_NAME = APP_NAME + +# CLOUDWATCH DASHBOARD CREATION +CREATE_DASHBOARD = 'True' # Create a dashboard in Cloudwatch for run +CLEAN_DASHBOARD = 'True' # Automatically remove dashboard at end of run with Monitor + +# REDUNDANCY CHECKS +CHECK_IF_DONE_BOOL = 'False' #True or False- should it check if there are a certain number of non-empty files and delete the job if yes? +EXPECTED_NUMBER_FILES = 7 #What is the number of files that trigger skipping a job? +MIN_FILE_SIZE_BYTES = 1 #What is the minimal number of bytes an object should be to "count"? +NECESSARY_STRING = '' #Is there any string that should be in the file name to "count"? + +# PLUGINS +USE_PLUGINS = 'False' +UPDATE_PLUGINS = 'False' +PLUGINS_COMMIT = '' # What commit or version tag do you want to check out? +INSTALL_REQUIREMENTS = 'False' +REQUIREMENTS_FILE = '' # Path within the plugins repo to a requirements file diff --git a/example_project_CPG/demo_project_folder/workspace/ExampleCPG.cppipe b/example_project_CPG/demo_project_folder/workspace/ExampleCPG.cppipe new file mode 100644 index 0000000..c7bb048 --- /dev/null +++ b/example_project_CPG/demo_project_folder/workspace/ExampleCPG.cppipe @@ -0,0 +1,134 @@ +CellProfiler Pipeline: http://www.cellprofiler.org +Version:4 +DateRevision:426 +GitHash: +ModuleCount:7 +HasImagePlaneDetails:False + +LoadData:[module_num:1|svn_version:'Unknown'|variable_revision_number:6|show_window:True|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Input data file location:Default Input Folder sub-folder|Desktop/github/Distributed-CellProfiler/example_project_CPG/project_folder/workspace + Name of the file:load_data.csv + Load images based on this data?:Yes + Base image location:Default Input Folder| + Process just a range of rows?:No + Rows to process:1,100000 + Group images by metadata?:Yes + Select metadata tags for grouping:Well,Site + Rescale intensities?:Yes + +IdentifyPrimaryObjects:[module_num:2|svn_version:'Unknown'|variable_revision_number:15|show_window:True|notes:['Identify the nuclei from the DAPI image. ']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the input image:OrigDNA + Name the primary objects to be identified:Nuclei + Typical diameter of objects, in pixel units (Min,Max):15,90 + Discard objects outside the diameter range?:Yes + Discard objects touching the border of the image?:Yes + Method to distinguish clumped objects:Shape + Method to draw dividing lines between clumped objects:Intensity + Size of smoothing filter:10 + Suppress local maxima that are closer than this minimum allowed distance:5 + Speed up by using lower-resolution image to find local maxima?:Yes + Fill holes in identified objects?:After declumping only + Automatically calculate size of smoothing filter for declumping?:Yes + Automatically calculate minimum allowed distance between local maxima?:Yes + Handling of objects if excessive number of objects identified:Continue + Maximum number of objects:500 + Use advanced settings?:Yes + Threshold setting version:12 + Threshold strategy:Global + Thresholding method:Minimum Cross-Entropy + Threshold smoothing scale:1 + Threshold correction factor:1.0 + Lower and upper bounds on threshold:0.005,1 + Manual threshold:0.0 + Select the measurement to threshold with:None + Two-class or three-class thresholding?:Three classes + Log transform before thresholding?:No + Assign pixels in the middle intensity class to the foreground or the background?:Background + Size of adaptive window:10 + Lower outlier fraction:0.05 + Upper outlier fraction:0.05 + Averaging method:Mean + Variance method:Standard deviation + # of deviations:2 + Thresholding method:Otsu + +IdentifySecondaryObjects:[module_num:3|svn_version:'Unknown'|variable_revision_number:10|show_window:True|notes:['Identify the cells by using the nuclei as a "seed" region, then growing outwards until stopped by the image threshold or by a neighbor.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the input objects:Nuclei + Name the objects to be identified:Cells + Select the method to identify the secondary objects:Watershed - Image + Select the input image:OrigRNA + Number of pixels by which to expand the primary objects:10 + Regularization factor:0.05 + Discard secondary objects touching the border of the image?:No + Discard the associated primary objects?:No + Name the new primary objects:FilteredNuclei + Fill holes in identified objects?:Yes + Threshold setting version:12 + Threshold strategy:Global + Thresholding method:Otsu + Threshold smoothing scale:0 + Threshold correction factor:.7 + Lower and upper bounds on threshold:0.005,.6 + Manual threshold:0 + Select the measurement to threshold with:None + Two-class or three-class thresholding?:Three classes + Log transform before thresholding?:Yes + Assign pixels in the middle intensity class to the foreground or the background?:Foreground + Size of adaptive window:10 + Lower outlier fraction:0.05 + Upper outlier fraction:0.05 + Averaging method:Mean + Variance method:Standard deviation + # of deviations:2 + Thresholding method:Otsu + +IdentifyTertiaryObjects:[module_num:4|svn_version:'Unknown'|variable_revision_number:3|show_window:True|notes:['Identify the cytoplasm by "subtracting" the nuclei objects from the cell objects.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the larger identified objects:Cells + Select the smaller identified objects:Nuclei + Name the tertiary objects to be identified:Cytoplasm + Shrink smaller object prior to subtraction?:Yes + +MeasureObjectSizeShape:[module_num:5|svn_version:'Unknown'|variable_revision_number:3|show_window:True|notes:['Measure morphological features from the cell, nuclei and cytoplasm objects.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select object sets to measure:Cells, Nuclei, Cytoplasm + Calculate the Zernike features?:Yes + Calculate the advanced features?:No + +MeasureObjectIntensity:[module_num:6|svn_version:'Unknown'|variable_revision_number:4|show_window:True|notes:['Measure intensity features from nuclei, cell, and cytoplasm objects in all channels']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select images to measure:OrigAGP, OrigDNA, OrigER, OrigMito, OrigRNA + Select objects to measure:Nuclei, Cells, Cytoplasm + +ExportToSpreadsheet:[module_num:7|svn_version:'Unknown'|variable_revision_number:13|show_window:True|notes:['Export any measurements to a comma-delimited file (.csv). The measurements made for the nuclei, cell and cytoplasm objects will be saved to separate .csv files, in addition to the per-image .csv’s.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the column delimiter:Comma (",") + Add image metadata columns to your object data file?:No + Add image file and folder names to your object data file?:No + Select the measurements to export:No + Calculate the per-image mean values for object measurements?:No + Calculate the per-image median values for object measurements?:No + Calculate the per-image standard deviation values for object measurements?:No + Output file location:Default Output Folder|. + Create a GenePattern GCT file?:No + Select source of sample row name:Metadata + Select the image to use as the identifier:None + Select the metadata to use as the identifier:None + Export all measurement types?:Yes + Press button to select measurements:None|None + Representation of Nan/Inf:NaN + Add a prefix to file names?:No + Filename prefix:MyExpt_ + Overwrite existing files without warning?:Yes + Data to export:Image + Combine these object measurements with those of the previous object?:No + File name:Image.csv + Use the object name for the file name?:No + Data to export:Nuclei + Combine these object measurements with those of the previous object?:No + File name:Nuclei.csv + Use the object name for the file name?:No + Data to export:Cells + Combine these object measurements with those of the previous object?:No + File name:Cells.csv + Use the object name for the file name?:No + Data to export:Cytoplasm + Combine these object measurements with those of the previous object?:No + File name:Cytoplasm.csv + Use the object name for the file name?:No diff --git a/example_project_CPG/demo_project_folder/workspace/load_data.csv b/example_project_CPG/demo_project_folder/workspace/load_data.csv new file mode 100644 index 0000000..ab86644 --- /dev/null +++ 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0000000..ced422b --- /dev/null +++ b/example_project_CPG/files/exampleCPGJob.json @@ -0,0 +1,15 @@ +{ + "_comment1": "Paths in this file are relative to the root of your S3 bucket", + "pipeline": "demo_project_folder/workspace/ExampleCPG.cppipe", + "data_file": "demo_project_folder/workspace/load_data.csv", + "input": "demo_project_folder/workspace/", + "output": "demo_project_folder/output", + "output_structure": "Metadata_Well-Metadata_Site", + "_comment2": "The following groups are tasks, and each will be run in parallel", + "groups": [ + {"Metadata": "Metadata_Well=A01,Metadata_Site=1"}, + {"Metadata": "Metadata_Well=A01,Metadata_Site=2"}, + {"Metadata": "Metadata_Well=A02,Metadata_Site=1"} + ] +} + diff --git a/run.py b/run.py index 371a93f..6ef6a05 100644 --- a/run.py +++ b/run.py @@ -11,6 +11,7 @@ # Back compatability with old config versions SOURCE_BUCKET = 'False' +WORKSPACE_BUCKET = 'False' UPLOAD_FLAGS = 'False' UPDATE_PLUGINS = 'False' CREATE_DASHBOARD = 'False' @@ -124,7 +125,7 @@ def generate_task_definition(AWS_PROFILE): {"name": "NECESSARY_STRING", "value": NECESSARY_STRING}, {"name": "DOWNLOAD_FILES", "value": DOWNLOAD_FILES}, ] - if SOURCE_BUCKET.lower()=='true': + if SOURCE_BUCKET.lower()!='false': task_definition['containerDefinitions'][0]['environment'] += [ { 'name': 'SOURCE_BUCKET', @@ -134,7 +135,13 @@ def generate_task_definition(AWS_PROFILE): 'name': 'DESTINATION_BUCKET', 'value': DESTINATION_BUCKET }] - if UPLOAD_FLAGS.lower()=='true': + if WORKSPACE_BUCKET.lower()!='false': + task_definition['containerDefinitions'][0]['environment'] += [ + { + 'name': 'WORKSPACE_BUCKET', + 'value': WORKSPACE_BUCKET + }] + if UPLOAD_FLAGS.lower()!='false': task_definition['containerDefinitions'][0]['environment'] += [ { 'name': 'UPLOAD_FLAGS', @@ -829,3 +836,4 @@ def monitor(cheapest=False): startCluster() elif sys.argv[1] == 'monitor': monitor() + \ No newline at end of file diff --git a/worker/cp-worker.py b/worker/cp-worker.py index f93424e..b2a1c74 100644 --- a/worker/cp-worker.py +++ b/worker/cp-worker.py @@ -23,6 +23,10 @@ DESTINATION_BUCKET = os.environ['AWS_BUCKET'] else: DESTINATION_BUCKET = os.environ['DESTINATION_BUCKET'] +if 'WORKSPACE_BUCKET' not in os.environ: + WORKSPACE_BUCKET = os.environ['SOURCE_BUCKET'] +else: + WORKSPACE_BUCKET = os.environ['WORKSPACE_BUCKET'] if 'UPLOAD_FLAGS' in os.environ: UPLOAD_FLAGS = os.environ['UPLOAD_FLAGS'] else: @@ -39,11 +43,11 @@ else: USE_PLUGINS = os.environ['USE_PLUGINS'] if 'NECESSARY_STRING' not in os.environ: - NECESSARY_STRING = False + NECESSARY_STRING = '' else: NECESSARY_STRING = os.environ['NECESSARY_STRING'] if 'DOWNLOAD_FILES' not in os.environ: - DOWNLOAD_FILES = False + DOWNLOAD_FILES = 'False' else: DOWNLOAD_FILES = os.environ['DOWNLOAD_FILES'] @@ -162,97 +166,112 @@ def runCellProfiler(message): bucketlist=s3client.list_objects(Bucket=DESTINATION_BUCKET,Prefix=f'{remoteOut}/') objectsizelist=[k['Size'] for k in bucketlist['Contents']] objectsizelist = [i for i in objectsizelist if i >= MIN_FILE_SIZE_BYTES] - if NECESSARY_STRING: - if NECESSARY_STRING != '': - objectsizelist = [i for i in objectsizelist if NECESSARY_STRING in i] + if NECESSARY_STRING != '': + objectsizelist = [i for i in objectsizelist if NECESSARY_STRING in i] if len(objectsizelist)>=int(EXPECTED_NUMBER_FILES): printandlog('File not run due to > expected number of files',logger) logger.removeHandler(watchtowerlogger) return 'SUCCESS' except KeyError: #Returned if that folder does not exist pass - - data_file_path = os.path.join(DATA_ROOT,message['data_file']) downloaded_files = [] - # Optional- download files - if DOWNLOAD_FILES: - if DOWNLOAD_FILES.lower() == 'true': - if not os.path.exists(localIn): - os.mkdir(localIn) - s3 = boto3.resource('s3') - if message['data_file'][-3:]=='.csv': - printandlog('Figuring which files to download', logger) - import pandas - csv_in = pandas.read_csv(data_file_path) - csv_in=csv_in.astype('str') - #Figure out what metadata fields we need in this experiment, as a dict - if type(message['Metadata'])==dict: - filter_dict = message['Metadata'] + # Optional - download all files, bypass S3 mounting + if DOWNLOAD_FILES.lower() == 'true': + # Download load data file and image files + data_file_path = os.path.join(localIn,message['data_file']) + printandlog(f"Downloading {message['data_file']} from {WORKSPACE_BUCKET}", logger) + csv_insubfolders = message['data_file'].split('/') + subfolders = '/'.join((csv_insubfolders)[:-1]) + if not os.path.exists(os.path.join(localIn,subfolders)): + os.makedirs(os.path.join(localIn,subfolders), exist_ok=True) + s3 = boto3.resource('s3') + s3.meta.client.download_file(WORKSPACE_BUCKET, message['data_file'], data_file_path) + if message['data_file'][-4:]=='.csv': + printandlog('Figuring which files to download', logger) + import pandas + csv_in = pandas.read_csv(data_file_path) + csv_in=csv_in.astype('str') + #Figure out what metadata fields we need in this experiment, as a dict + if type(message['Metadata'])==dict: + filter_dict = message['Metadata'] + else: + filter_dict = {} + for eachMetadata in message['Metadata'].split(','): + filterkey, filterval = eachMetadata.split('=') + filter_dict[filterkey] = filterval + #Filter our CSV to just the rows CellProfiler will process, so that we can download only what we need + for eachfilter in filter_dict.keys(): + csv_in = csv_in[csv_in[eachfilter] == filter_dict[eachfilter]] + if len(csv_in) < 1: + printandlog('WARNING: All rows filtered out of csv before download. Check your Metadata.',logger) + logger.removeHandler(watchtowerlogger) + import shutil + shutil.rmtree(localOut, ignore_errors=True) + return 'INPUT_PROBLEM' + #Figure out the actual file names and get them + channel_list = [x.split('FileName_')[1] for x in csv_in.columns if 'FileName' in x] + printandlog(f'Downloading files for channels {channel_list}', logger) + for channel in channel_list: + for field in range(csv_in.shape[0]): + full_old_file_name = os.path.join(list(csv_in[f'PathName_{channel}'])[field],list(csv_in[f'FileName_{channel}'])[field]) + prefix_on_bucket = full_old_file_name.split(DATA_ROOT)[1][1:] + new_file_name = os.path.join(localIn,prefix_on_bucket) + if not os.path.exists(os.path.split(new_file_name)[0]): + os.makedirs(os.path.split(new_file_name)[0]) + printandlog(f'Made directory {os.path.split(new_file_name)[0]}',logger) + if not os.path.exists(new_file_name): + s3.meta.client.download_file(SOURCE_BUCKET,prefix_on_bucket,new_file_name) + downloaded_files.append(new_file_name) + printandlog(f'Downloaded {str(len(downloaded_files))} files',logger) + import random + newtag = False + while newtag == False: + tag = str(random.randint(100000,999999)) #keep files from overwriting one another + local_data_file_path = os.path.join(localIn,tag,os.path.split(data_file_path)[1]) + if not os.path.exists(local_data_file_path): + if not os.path.exists(os.path.split(local_data_file_path)[0]): + os.makedirs(os.path.split(local_data_file_path)[0]) + csv_in = pandas.read_csv(data_file_path) + csv_in.replace(DATA_ROOT,localIn,regex=True, inplace=True) + csv_in.to_csv(local_data_file_path,index=False) + print('Wrote updated CSV') + newtag = True else: - filter_dict = {} - for eachMetadata in message['Metadata'].split(','): - filterkey, filterval = eachMetadata.split('=') - filter_dict[filterkey] = filterval - #Filter our CSV to just the rows CellProfiler will process, so that we can download only what we need - for eachfilter in filter_dict.keys(): - csv_in = csv_in[csv_in[eachfilter] == filter_dict[eachfilter]] - if len(csv_in) <= 1: - printandlog('WARNING: All rows filtered out of csv before download. Check your Metadata.') - #Figure out the actual file names and get them - channel_list = [x.split('FileName_')[1] for x in csv_in.columns if 'FileName' in x] - printandlog('Downloading files', logger) - for channel in channel_list: - for field in range(csv_in.shape[0]): - full_old_file_name = os.path.join(list(csv_in[f'PathName_{channel}'])[field],list(csv_in[f'FileName_{channel}'])[field]) - prefix_on_bucket = full_old_file_name.split(DATA_ROOT)[1][1:] - new_file_name = os.path.join(localIn,prefix_on_bucket) - if not os.path.exists(os.path.split(new_file_name)[0]): - os.makedirs(os.path.split(new_file_name)[0]) - printandlog(f'made directory {os.path.split(new_file_name)[0]}',logger) - if not os.path.exists(new_file_name): - s3.meta.client.download_file(AWS_BUCKET,prefix_on_bucket,new_file_name) - downloaded_files.append(new_file_name) - printandlog(f'Downloaded {str(len(downloaded_files))} files',logger) - import random - newtag = False - while newtag == False: - tag = str(random.randint(100000,999999)) #keep files from overwriting one another - local_data_file_path = os.path.join(localIn,tag,os.path.split(data_file_path)[1]) - if not os.path.exists(local_data_file_path): - if not os.path.exists(os.path.split(local_data_file_path)[0]): - os.makedirs(os.path.split(local_data_file_path)[0]) - csv_in = pandas.read_csv(data_file_path) - csv_in.replace(DATA_ROOT,localIn,regex=True, inplace=True) - csv_in.to_csv(local_data_file_path,index=False) - print('Wrote updated CSV') - newtag = True - else: - newtag = False - data_file_path = local_data_file_path - elif message['data_file'][-3:]=='.txt': - printandlog('Downloading files', logger) - with open(data_file_path, 'r') as f: - for file_path in f: - prefix_on_bucket = file_path.split(DATA_ROOT)[1][1:] - new_file_name = os.path.join(localIn,prefix_on_bucket) - if not os.path.exists(os.path.split(new_file_name)[0]): - os.makedirs(os.path.split(new_file_name)[0]) - printandlog(f'made directory {os.path.split(new_file_name)[0]}',logger) - if not os.path.exists(new_file_name): - s3.meta.client.download_file(AWS_BUCKET,prefix_on_bucket,new_file_name) - downloaded_files.append(new_file_name) - printandlog(f'Downloaded {str(len(downloaded_files))} files',logger) + newtag = False + data_file_path = local_data_file_path + elif message['data_file'][-4:]=='.txt': + printandlog('Downloading files', logger) + with open(data_file_path, 'r') as f: + for file_path in f: + prefix_on_bucket = file_path.split(DATA_ROOT)[1][1:] + new_file_name = os.path.join(localIn,prefix_on_bucket) + if not os.path.exists(os.path.split(new_file_name)[0]): + os.makedirs(os.path.split(new_file_name)[0]) + printandlog(f'made directory {os.path.split(new_file_name)[0]}',logger) + if not os.path.exists(new_file_name): + s3.meta.client.download_file(SOURCE_BUCKET,prefix_on_bucket,new_file_name) + downloaded_files.append(new_file_name) + printandlog(f'Downloaded {str(len(downloaded_files))} files',logger) + else: + printandlog("Couldn't parse data file for file download. Not supported input of .csv or .txt",logger) + # Download pipeline and update pipeline path in message + printandlog(f"Downloading {message['pipeline']} from {WORKSPACE_BUCKET}", logger) + pipepath = os.path.join(localIn, message['pipeline'].split('/')[-1]) + s3.meta.client.download_file(WORKSPACE_BUCKET, message['pipeline'], pipepath) + else: + data_file_path = os.path.join(DATA_ROOT,message['data_file']) + pipepath = os.path.join(DATA_ROOT,message["pipeline"]) # Build and run CellProfiler command cpDone = f'{localOut}/cp.is.done' if message['data_file'][-4:]=='.csv': - cmd = f'cellprofiler -c -r -p {DATA_ROOT}/{message["pipeline"]} -i {DATA_ROOT}/{message["input"]} -o {localOut} -d {cpDone} --data-file={data_file_path} -g {message["Metadata"]}' + cmd = f'cellprofiler -c -r -p {pipepath} -i {DATA_ROOT}/{message["input"]} -o {localOut} -d {cpDone} --data-file={data_file_path} -g {message["Metadata"]}' elif message['data_file'][-3:]=='.h5': - cmd = f'cellprofiler -c -r -p {DATA_ROOT}/{message["pipeline"]} -i {DATA_ROOT}/{message["input"]} -o {localOut} -d {cpDone} -g {message["Metadata"]}' + cmd = f'cellprofiler -c -r -p {pipepath} -i {DATA_ROOT}/{message["input"]} -o {localOut} -d {cpDone} -g {message["Metadata"]}' elif message['data_file'][-4:]=='.txt': - cmd = f'cellprofiler -c -r -p {DATA_ROOT}/{message["pipeline"]} -i {DATA_ROOT}/{message["input"]} -o {localOut} -d {cpDone} --file-list={data_file_path} -g {message["Metadata"]}' + cmd = f'cellprofiler -c -r -p {pipepath} -i {DATA_ROOT}/{message["input"]} -o {localOut} -d {cpDone} --file-list={data_file_path} -g {message["Metadata"]}' else: printandlog("Didn't recognize input file",logger) if USE_PLUGINS.lower() == 'true': @@ -343,4 +362,4 @@ def main(): logging.basicConfig(level=logging.INFO) print('Worker started') main() - print('Worker finished') + print('Worker finished') \ No newline at end of file