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DNA concentration using SpeedVac
DNA_library_prep_(parchman).md
LICENSE
README.md
Silica bead rejuvenation protocol.md
burt_dna_extraction.md
casting_gels.md
cleaning_beads.md
dna_concentration.md
gel_extraction_protocol.md
high_throughput_bead_autoclave_protocol.md
quantitating_dna.md
running_gels.md
single_dna_extraction.md
water_autoclave_protocol.md

README.md

Protocols

This repository holds protocols for the lab of Andrew J. Eckert (EckertLab) at Virginia Commonwealth University. Everyone in the Eckert Lab is an admin for this organization, but if you have trouble please let us know.

Printing

If you want to print the protocols, use the awesome GitPrint extension or bookmarklet.


Even if you're not in the lab, pull requests are welcome and encouraged.

Protocols specific to publications

For a specific set of protocols regarding a particular publication, please email us. Most of our recent work will be done using ddRadSeq, the progression of laboratory efforts is as follows:

  1. DNA extraction via Qiagen DNeasy 96 Plant Kit
  2. High throughput multiplexed DNA library preparation (sensu Parchman 2012)
  3. Fragment size selection and prep for sequencing