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GTDB-Tk is a software toolkit for assigning objective taxonomic classifications to bacterial and archaeal genomes based on the Genome Database Taxonomy (GTDB). It is designed to work with recent advances that allow hundreds or thousands of metagenome-assembled genomes (MAGs) to be obtained directly from environmental samples. It can also be applied to isolate and single-cell genomes. The GTDB-Tk is open source and released under the GNU General Public License (Version 3).

Notifications about GTDB-Tk releases will be available through the GTDB Twitter account and the GTDB Announcements Forum.

Please post questions and issues related to GTDB-Tk on the Issues section of the GitHub repository. Questions related to the GTDB can be posted on the GTDB Forum or sent to the GTDB team.

πŸš€ Getting started

Be sure to check the hardware requirements, then choose your preferred method:

πŸ“– Documentation

Documentation for GTDB-Tk can be found here.

✨ New Features

GTDB-Tk v2.3.0+ includes the following new features:

  • New functionality convert_to_species function to convert GTDB genome IDs to GTDB species names

πŸ“ˆ Performance

Using ANI screen "can" reduce computation by >50%, although it depends on the set of input genomes. A set of input genomes consisting primarily of new species will not benefit from ANI screen as much as a set of genomes that are largely assigned to GTDB species clusters. In the latter case, the ANI screen will reduce the number of genomes that need to be classified by pplacer which reduces computation time substantially (between 25% and 60% in our testing).

πŸ“š References

GTDB-Tk is described in:

The Genome Taxonomy Database (GTDB) is described in:

We strongly encourage you to cite the following 3rd party dependencies:

Copyright 2017 Pierre-Alain Chaumeil. See LICENSE for further details.