a script which automatically creates "positive gates" using control samples' properties.
HOW TO USE THIS FLOWJO SCRIPT: Download and save this script, and/or the template + data. In FlowJo version 10.0.8, load in your Controls and Samples data into groups CONTROLS and SAMPLES, respectively. The script assumes these groups will own a gate called "Live" and all calculations and results will be performed at that gating level.
Go to Tools - and Open the Script Editor. At the bottom of the script editor, click the 'Open Script' icon, then navigate to the script and open it, and then click 'Run' in the Script Editor.
Controls' Live gate will be assessed for the percentile of FITC distribution, and the most inclusive one will be used to create a FITC_A gate. This gate will then be applied to all samples in the SAMPLES group, at the Live gate level.
Note: If you edit the script, remember to save it (using the button next to 'open script')
FOR MORE INFORMATION: http://docs.flowjo.com/d2/advanced-features/script-editor/
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