a script which automatically creates "positive gates" using control samples' properties.
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ControlBasedGating
LICENSE
README.md
template.wspt

README.md

ControlBasedInclusionGating

a script which automatically creates "positive gates" using control samples' properties.

  1. HOW TO USE THIS FLOWJO SCRIPT: Download and save this script, and/or the template + data. In FlowJo version 10.0.8, load in your Controls and Samples data into groups CONTROLS and SAMPLES, respectively. The script assumes these groups will own a gate called "Live" and all calculations and results will be performed at that gating level.

  2. Go to Tools - and Open the Script Editor. At the bottom of the script editor, click the 'Open Script' icon, then navigate to the script and open it, and then click 'Run' in the Script Editor.

  3. Controls' Live gate will be assessed for the percentile of FITC distribution, and the most inclusive one will be used to create a FITC_A gate. This gate will then be applied to all samples in the SAMPLES group, at the Live gate level.

  4. Note: If you edit the script, remember to save it (using the button next to 'open script')

  5. FOR MORE INFORMATION: http://docs.flowjo.com/d2/advanced-features/script-editor/

  6. DISCLAIMER: Software distributed under the APACHE 2.0 License is distributed on an "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the License for the specific language governing permissions and limitations under the License.

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  1. the software will meet your requirements.
  2. the software will be uninterrupted, timely, secure or error-free.
  3. the results that may be obtained from the use of the software will be effective, accurate or reliable.
  4. the quality of the software will meet your expectations.
  5. any errors in the software obtained from the FlowJo Exchange web site will be corrected.

The use of the software downloaded through the FlowJo Exchange site is done at your own discretion and risk and with agreement that you will be solely responsible for any damage to your computer system or loss of data that results from such activities. No advice or information, whether oral or written, obtained by you from FlowJo, LLC, its its website, its contributors, employees, or contractors shall create any warranty for the software.