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#!perl
use Config;
use File::Basename qw(&basename &dirname);
use Cwd;
$origdir = cwd;
chdir dirname($0);
$file = basename($0, '.PL','.PLS');
$file .= $^O eq 'VMS' ? '.com' : '.pl';
open OUT,">$file" or die "Can't create $file: $!";
print "Extracting $file (with variable substitutions)\n";
print OUT <<"!GROK!THIS!";
$Config{startperl}
!GROK!THIS!
# In the following, perl variables are not expanded during extraction.
print OUT <<'!NO!SUBS!';
use constant ACEDB => 'sace://aceserver.cshl.org:2005';
use strict;
use Ace;
my @framework = qw(mex-3 spe-15 lin-17 unc-11 dhc-1 unc-40 smg-5
unc-13 unc-29 eat-16 lin-11 spe-9 par-6 unc-59 unc-54 mab-9 lin-42
sri-71 smu-2 vab-1 bli-2 dpy-10 him-14 mig-5 unc-4 bli-1 sqt-1 rol-1
his-14 unc-52 unc-45 par-2 let-805 sel-8 mab-21 daf-4 sma-3 lin-39
unc-32 tax-4 ced-9 tra-1 nob-1 daf-1 ced-2 lin-1 unc-17 dpy-13 unc-5
smg-7 dif-1 lin-49 elt-1 daf-14 dpy-20 dpy-26 unc-30 tra-3 sup-24
rho-1 egl-8 unc-60 srh-36 apx-1 unc-62 let-418 dpy-11 let-413 sel-9
unc-42 egl-9 sma-1 sqt-3 odr-3 hda-1 unc-76 gcy-20 skr-5 par-4 unc-51
egl-17 lim-6 fox-1 fax-1 lon-2 unc-97 unc-6 unc-18 mec-10 sop-1 mab-18
sdc-2 odr-7 unc-9 unc-3 gas-1 ace-1);
my %framework = map {$_=>1} @framework;
my %framework_seen = ();
my $USAGE = <<USAGE;
This script massages the Wormbase GFF files located at
ftp://www.wormbase.org/pub/wormbase/GENE_DUMPS into a version of the
GFF format suitable for display by the generic genome browser. It
mainly adds comments to the annotations and designates certain
well-spaced genetic loci as framework landmarks.
This script requires the AcePerl distribution, which is available on
CPAN (look for the "Ace" module).
To use this script, get the WormBase GFF files from the FTP site
listed above and place them in a directory. It might be a good idea
to name the directory after the current release, such as WS61. You do
not need to uncompress the files.
Then give that directory as the argument to this script and capture
the script's output to a file:
% process_wormbase.pl ./WS61 > wormbase.gff
It may take a while before you see output from this script, since it
must first fetch gene and protein database from the remote AceDB
running at www.wormbase.org.
The wormbase.gff file can then be loaded into a Bio::DB::GFF database
using the following command:
% bulk_load_gff.pl -d <databasename> wormbase.gff
USAGE
;
#'
die $USAGE if $ARGV[0]=~/^-?-h/i;
my $db = Ace->connect(-url=>ACEDB,
-query_timeout=>500) or die "Can't open ace database:",Ace->error;
if (-d $ARGV[0]) {
@ARGV = <$ARGV[0]/*.gff.gz>;
}
@ARGV || die $USAGE;
foreach (@ARGV) { # GFF FILES
$_ = "gunzip -c $_ |" if /\.gz$/;
}
my (%NOTES,%LOCUS,%GENBANK,%CONFIRMED,%ORFEOME);
get_confirmed($db,\%CONFIRMED);
get_genbank($db,\%GENBANK);
get_loci($db,\%LOCUS);
get_transcripts($db,\%LOCUS);
get_notes($db,\%NOTES);
get_orfeome($db,\%ORFEOME);
while (<>) {
chomp;
next if /^\#/;
my ($ref,$source,$method,$start,$stop,$score,$strand,$phase,$group) = split /\t/;
next if $source eq 'assembly_tag'; # don't want 'em, don't need 'em
next if $method eq 'HOMOL_GAP'; # don't want that neither
$ref =~ s/^CHROMOSOME_//;
$group =~ s/CHROMOSOME_//;
$source ='' if $source eq '*UNKNOWN*';
if ($method =~ /Transcript|CDS/
&& ($source =~ /curated|RNA|miRNA/)
&& $group =~ /Transcript|CDS "(\w+\.\d+[a-z]?)"/) {
my @notes;
push @notes,map { qq(Note "$_") } @{$NOTES{$1}} if $NOTES{$1};
push @notes,map { qq(Note "$_") } @{$LOCUS{$1}} if $LOCUS{$1};
push @notes,qq(Confirmed_by "$CONFIRMED{$1}") if $CONFIRMED{$1};
$group = join ' ; ',$group,@notes;
if (my $loci = $LOCUS{$1}) {
foreach (@$loci) {
print join("\t",$ref,$source,'gene',$start,$stop,$score,$strand,$phase,"Locus $_"),"\n";
print join("\t",$ref,'framework','gene',$start,$stop,$score,$strand,$phase,"Locus $_"),"\n"
if $framework{$_} && !$framework_seen{$_}++;
}
}
}
if ($method eq 'region' && $source eq 'Genomic_canonical' && $group =~ /Sequence "(\w+)"/) {
if (my $accession = $GENBANK{$1}) {
$group .= qq( ; Note "Genbank $accession");
print join("\t",$ref,'Genbank',$method,$start,$stop,$score,$strand,$phase,"Genbank \"$accession\""),"\n";
}
}
if ($method eq 'intron' && $source =~ /^tRNAscan/) {
next; # messing up tRNA scanning
}
if ($method eq 'PCR_product' && $source eq 'Orfeome' && $group =~ /PCR_product "([^\"]+)"/) {
my $amp = $ORFEOME{$1};
$group .= qq( ; Amplified $amp) if defined $amp;
}
# fix variant fields: Variant "T" => Note "T"
$group =~ s/(?:Variant|Insert) "(\w+)"/Note "$1"/;
# fix UTR fields
if ($group =~ /UTR "([35])_UTR:(\S+)"/) {
$method = 'UTR';
$source = "curated";
$group = qq(CDS "$2");
}
print join("\t",$ref,$source,$method,$start,$stop,$score,$strand,$phase,$group),"\n";
}
sub get_loci {
my ($db,$hash) = @_; # hash keys are predicted gene names, values are one or more loci names
my @genes = $db->fetch(-query=>'find Locus CDS',-filltag=>'CDS');
foreach my $obj (@genes) {
my @genomic = $obj->CDS or next;
foreach (@genomic) {
push @{$hash->{$_}},$obj;
}
}
}
sub get_transcripts {
my ($db,$hash) = @_; # hash keys are predicted gene names, values are one or more loci names
my @genes = $db->fetch(-query=>'find Locus Transcript',-filltag=>'Transcript');
foreach my $obj (@genes) {
my @genomic = $obj->Transcript or next;
foreach (@genomic) {
push @{$hash->{$_}},$obj;
}
}
}
sub get_notes {
my ($db,$hash) = @_; # hash keys are predicted gene names, values are one or more brief identifications
my @genes = $db->fetch(-query=>'find CDS Brief_identification',-filltag=>'Brief_identification');
# Should probably also look for notes attached to sequences, yes? As before...
push (@genes,$db->fetch(-query=>'find Sequence Brief_identification',-filltag=>'Brief_identification'));
foreach my $obj (@genes) {
my @notes = $obj->Brief_identification or next;
$hash->{$obj} = \@notes;
}
}
sub get_genbank {
my ($db,$hash) = @_; # hash keys are cosmid names, values are genbank accessions (1 to 1)
my @cosmids = $db->fetch(-query=>'find Genome_Sequence Database',-filltag=>'Database');
for my $cosmid (@cosmids) {
my @dbs = $cosmid->Database;
foreach (@dbs) {
foreach my $col ($_->col) {
next unless $col eq 'NDB_AC';
$hash->{$cosmid} = $col->right;
}
}
}
}
sub get_confirmed {
my ($db,$hash) = @_; # hash keys are predicted gene names, values are confirmation type
my @confirmed = $db->fetch(-query=>'find Sequence Confirmed_by',-filltag=>'Confirmed_by');
foreach my $obj (@confirmed) {
my $confirmed_by = $obj->Confirmed_by || 'Unknown';
$hash->{$obj} = $confirmed_by;
}
}
sub get_orfeome {
my ($db,$hash) = @_;
my @mv_primers = $db->fetch(-query=>'find PCR_Product mv*',-filltag=>'Amplified');
for my $obj (@mv_primers) {
my $amplified = $obj->Amplified;
$hash->{$obj} = $amplified;
}
}
__END__
=head1 NAME
process_wormbase.pl - Massage WormBase GFF files into a version suitable for the Generic Genome Browser
=head1 SYNOPSIS
% process_wormbase.pl ./WS121 > wormbase.gff
=head1 DESCRIPTION
This script massages the Wormbase GFF files located at
ftp://www.wormbase.org/pub/wormbase/GENE_DUMPS into a version of the
GFF format suitable for display by the generic genome browser. It is
current for data releases WS121 and beyond. It mainly adds comments to
the annotations and designates certain well-spaced genetic loci as
framework landmarks.
This script requires the AcePerl distribution, which is available on
CPAN (look for the "Ace" module).
To use this script, get the WormBase GFF files from the FTP site
listed above and place them in a directory. It might be a good idea
to name the directory after the current release, such as WS61. You do
not need to uncompress the files.
Then give that directory as the argument to this script and capture
the script's output to a file:
% process_wormbase.pl ./WS121 > wormbase.gff
It may take a while before you see output from this script, since it
must first fetch gene and protein database from the remote AceDB
running at www.wormbase.org.
The wormbase.gff file can then be loaded into a Bio::DB::GFF database
using the following command:
% bulk_load_gff.pl -d <databasename> wormbase.gff
=head1 SEE ALSO
L<Bio::DB::GFF>, L<bulk_load_gff.pl>, L<load_gff.pl>
=head1 AUTHOR
Lincoln Stein <lstein@cshl.org>.
Copyright (c) 2002 Cold Spring Harbor Laboratory
This library is free software; you can redistribute it and/or modify
it under the same terms as Perl itself. See DISCLAIMER.txt for
disclaimers of warranty.
=cut
!NO!SUBS!
close OUT or die "Can't close $file: $!";
chmod 0755, $file or die "Can't reset permissions for $file: $!\n";
exec("$Config{'eunicefix'} $file") if $Config{'eunicefix'} ne ':';
chdir $origdir;
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