diff --git a/python/docs/source/migration_guide/pyspark_upgrade.rst b/python/docs/source/migration_guide/pyspark_upgrade.rst
index 992101734ff68..53d82554d7525 100644
--- a/python/docs/source/migration_guide/pyspark_upgrade.rst
+++ b/python/docs/source/migration_guide/pyspark_upgrade.rst
@@ -42,6 +42,7 @@ Upgrading from PySpark 3.5 to 4.0
 * In Spark 4.0, ``squeeze`` parameter from ``ps.read_csv`` and ``ps.read_excel`` has been removed from pandas API on Spark.
 * In Spark 4.0, ``null_counts`` parameter from ``DataFrame.info`` has been removed from pandas API on Spark, use ``show_counts`` instead.
 * In Spark 4.0, the result of ``MultiIndex.append`` does not keep the index names from pandas API on Spark.
+* In Spark 4.0, ``col_space`` parameter from ``DataFrame.to_latex`` and ``Series.to_latex`` has been removed from pandas API on Spark.
 
 
 Upgrading from PySpark 3.3 to 3.4
diff --git a/python/pyspark/pandas/frame.py b/python/pyspark/pandas/frame.py
index 3aebbd6542731..fedbfcdb5f31a 100644
--- a/python/pyspark/pandas/frame.py
+++ b/python/pyspark/pandas/frame.py
@@ -2473,7 +2473,6 @@ def to_latex(
         self,
         buf: Optional[IO[str]] = None,
         columns: Optional[List[Name]] = None,
-        col_space: Optional[int] = None,
         header: bool = True,
         index: bool = True,
         na_rep: str = "NaN",
@@ -2509,11 +2508,6 @@ def to_latex(
             Buffer to write to. If None, the output is returned as a string.
         columns : list of label, optional
             The subset of columns to write. Writes all columns by default.
-        col_space : int, optional
-            The minimum width of each column.
-
-            .. deprecated:: 3.4.0
-
         header : bool or list of str, default True
             Write out the column names. If a list of strings is given, it is assumed to be aliases
             for the column names.
@@ -2590,11 +2584,6 @@ def to_latex(
         \bottomrule
         \end{tabular}
         """
-        warnings.warn(
-            "Argument `col_space` will be removed in 4.0.0.",
-            FutureWarning,
-        )
-
         args = locals()
         psdf = self
         return validate_arguments_and_invoke_function(
diff --git a/python/pyspark/pandas/series.py b/python/pyspark/pandas/series.py
index 863e98c42ead4..5ef814eea367b 100644
--- a/python/pyspark/pandas/series.py
+++ b/python/pyspark/pandas/series.py
@@ -1662,7 +1662,6 @@ def to_latex(
         self,
         buf: Optional[IO[str]] = None,
         columns: Optional[List[Name]] = None,
-        col_space: Optional[int] = None,
         header: bool = True,
         index: bool = True,
         na_rep: str = "NaN",
@@ -1682,11 +1681,6 @@ def to_latex(
         multicolumn_format: Optional[str] = None,
         multirow: Optional[bool] = None,
     ) -> Optional[str]:
-        warnings.warn(
-            "Argument `col_space` will be removed in 4.0.0.",
-            FutureWarning,
-        )
-
         args = locals()
         psseries = self
         return validate_arguments_and_invoke_function(
diff --git a/python/pyspark/pandas/tests/test_dataframe_conversion.py b/python/pyspark/pandas/tests/test_dataframe_conversion.py
index d245b449d57d4..655c0970082f8 100644
--- a/python/pyspark/pandas/tests/test_dataframe_conversion.py
+++ b/python/pyspark/pandas/tests/test_dataframe_conversion.py
@@ -211,7 +211,6 @@ def test_to_latex(self):
         psdf = self.psdf
 
         self.assert_eq(psdf.to_latex(), pdf.to_latex())
-        self.assert_eq(psdf.to_latex(col_space=2), pdf.to_latex(col_space=2))
         self.assert_eq(psdf.to_latex(header=True), pdf.to_latex(header=True))
         self.assert_eq(psdf.to_latex(index=False), pdf.to_latex(index=False))
         self.assert_eq(psdf.to_latex(na_rep="-"), pdf.to_latex(na_rep="-"))
diff --git a/python/pyspark/pandas/tests/test_series_conversion.py b/python/pyspark/pandas/tests/test_series_conversion.py
index cbdb02db85aa0..757da1672500a 100644
--- a/python/pyspark/pandas/tests/test_series_conversion.py
+++ b/python/pyspark/pandas/tests/test_series_conversion.py
@@ -58,7 +58,6 @@ def test_to_latex(self):
         psser = self.psser
 
         self.assert_eq(psser.to_latex(), pser.to_latex())
-        self.assert_eq(psser.to_latex(col_space=2), pser.to_latex(col_space=2))
         self.assert_eq(psser.to_latex(header=True), pser.to_latex(header=True))
         self.assert_eq(psser.to_latex(index=False), pser.to_latex(index=False))
         self.assert_eq(psser.to_latex(na_rep="-"), pser.to_latex(na_rep="-"))