From 196ffc3088671d3a00c89fabc480e85d70e93131 Mon Sep 17 00:00:00 2001
From: Karoline Holler <63735546+KarolineHoller@users.noreply.github.com>
Date: Fri, 20 Nov 2020 18:23:29 +0100
Subject: [PATCH] Update README.md

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-# tomo-seq
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+# Find additional information for your own tomo-seq experiment. Or reproduce our results on zebrafish embryos.
+
+## How to run your experiment: 
+
+1. Primers for tomo-seq can be found in the file 'tomoseq_primer_sequences.csv'.
+
+2. A detailed protocol can be found here: Holler K and Junker JP, 2019.
+
+3. Map your sequenced experiment with 'mapping_tomoseq_data.sh'. Depended on the sequencing depth this can take some hours.
+
+4. Analyze the data in your favourite data mining language. We used R and appended our script to reproduce the figures of our paper. That file is named 'tomoseq_data_analysis_danioRerio.R'.