What are the strategies to overcome diffusion limitation in growing cells in suspension?
Diffusion limitation can be overcome in growing cells in suspension by implementing the following strategies:

1) Increase culture volume: By increasing the volume of the culture, the concentration of nutrients and oxygen can be kept at adequate levels, reducing the impact of diffusion limitation.

2) Stirring or agitation: Constant stirring or agitation can help to distribute nutrients and oxygen evenly throughout the culture, reducing the impact of diffusion limitation.

3) Oxygen-permeable membrane: By using an oxygen-permeable membrane, the oxygen levels can be regulated, helping to overcome diffusion limitation.

4) Microcarriers: Microcarriers can be used to support the growth of cells in suspension, providing a large surface area for cells to attach and access nutrients, which can help to overcome diffusion limitation.

5) Sparging: The process of sparging, where air or oxygen is bubbled through the culture, can help to increase the oxygen levels and reduce the impact of diffusion limitation.

6) Nutrient supplementation: Supplementing the culture with additional nutrients can help to maintain optimal nutrient levels, reducing the impact of diffusion limitation.
Ah, and just to be sure, what mediums are best suited for culture development? I hear agar is common for petri dish growth, but what of suspension?