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LFAQ v1.0.1

Contents

Overview

LFAQ is a novel algorithm for label-free absolute protein quantification, which can correct the biased MS intensities using the predicted peptide quantitative factors for all identified peptides.

Repository Contents

ExecutableFiles: The executable files of LFAQ
Sourcecode:The source codes of LFAQ
TestData: Real datasets to demo LFAQ

System Requirements

Hardware Requirements

  • 1 CPU processor at 2.4 GHz or higher
  • 2G RAM or higher
  • 100G of free hard disk space or higher

Software Requirements

  • Verified operating system (OS) versions (32-bit or 64-bit)
    • Windows 7 SP1
    • Windows 10
    • Windows Server 2008 R2
    • Windows Server 2012 R2
  • .NET Framework 4.5 or above from Microsoft, which can be downloaded from Micosoft.
  • Microsoft Visual C++ Redistributable for Visual Studio 2013: download from here.
  • R version 3.3.3 and above (for Windows) from R project
    • Download and install R from R-project
    • Set the path of RScript.exe as system environment variable
    • Install R package "ggplot2"
        install.packages("ggplot2")
    

Installation

Download the project files and Uncompress the zip package into a specified file folder. Double-click “LFAQ.exe” to run LFAQ. For detailed information, please see the file called "User Guide for LFAQ.pdf".

Documentation

This version of LFAQ is a full release, including source code and user guide documentation-"User Guide for LFAQ.pdf". As more releases occur, better documentation will be included.

The Latest Version

Details of the latest version can be found on the LFAQ project page under https://lfaq.github.io/LFAQ/.

Licensing

Please see the file called "LICENSE.md".

Contacts

For any questions involving LFAQ, please contact us.
Cheng Chang or ZhiQiang Gao

Acknowledgments

Dr. Cheng Chang - algorithm design and validation
ZhiQiang Gao   - software development and optimization

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a novel algorithm for label-free absolute protein quantification

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