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inconsistent between .fq.gz and .fastq? #4

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AMChalkie opened this issue Dec 2, 2019 · 5 comments
Closed

inconsistent between .fq.gz and .fastq? #4

AMChalkie opened this issue Dec 2, 2019 · 5 comments
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@AMChalkie
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@AMChalkie AMChalkie commented Dec 2, 2019

Strange as this may sound, I get different results when using a .fq.gz vs a .fastq file (when the .fastq is a zcat of original). I get different mappability results (0.018 ish vs the expected 85%).

Would be great if this was handled automatically and input from .fq.gz was handled correctly, or alternatively at least a warning that this might be what's happening.

Otherwise - very useful software thank you!

@hyunhwaj
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@hyunhwaj hyunhwaj commented Dec 2, 2019

Dear @AMChalkie,

The current version does not support gzipped FASTQ file, so you need to unzip the files. But the suggestion will be great for the CB^2. I will start to add the function soon.

Thank you,

Hyun-Hwan Jeong

@hyunhwaj hyunhwaj self-assigned this Dec 2, 2019
@hyunhwaj
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@hyunhwaj hyunhwaj commented Dec 12, 2019

@AMChalkie, the current github version supports gzipped FASTQ files. I wonder you are willing to test the version and let me know if you have any problem with that.

Thank you!

Hyun-Hwan Jeong

@AMChalkie
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@AMChalkie AMChalkie commented Dec 13, 2019

Yes that works fine now. I notice that mappability information is not printed during the process now, which I thought was a good feature previously.

@hyunhwaj
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@hyunhwaj hyunhwaj commented Dec 16, 2019

@AMChalkie you should see the message if you set the verbose parameter as TRUE.

Thank you,

Hyun-Hwan Jeong

@hyunhwaj
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@hyunhwaj hyunhwaj commented Dec 16, 2019

@AMChalkie, Can you test on it again? The version which you tested has an incompatibility issue with Windows OS. So, I made some changes and wonder the change doesn't cause any problem.

Thank you,

Hyun-Hwan Jeong

@hyunhwaj hyunhwaj closed this Dec 27, 2019
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