LIGER (Linked Inference of Genomic Experimental Relationships)
liger) is a package for integrating and analyzing multiple single-cell datasets, developed and maintained by the Macosko lab. It relies on integrative non-negative matrix factorization to identify shared and dataset-specific factors.
LIGER can be used to compare and contrast experimental datasets in a variety of contexts, for instance:
- Across experimental batches
- Across individuals
- Across sex
- Across tissues
- Across species (e.g., mouse and human)
- Across modalities (e.g., scRNAseq and spatial transcriptomics data, scMethylation, or scATAC-seq)
Once multiple datasets are integrated, the package provides functionality for further data exploration, analysis, and visualization. Users can:
- Identify clusters
- Find significant shared (and dataset-specific) gene markers
- Compare clusters with previously identified cell types
- Visualize clusters and gene expression using t-SNE and UMAP
We have also designed LIGER to interface with existing single-cell analysis packages, including Seurat.
For usage examples and guided walkthroughs, check the
vignettes directory of the repo.
liger is written in R and has a few other system requirements (Java) and recommended packages (umap in Python). To install the most recent development version, follow these instructions:
- Install R (>= 3.4)
- Install Rstudio (recommended)
- Make sure you have Java installed in your machine. Check by typing
java -versioninto Terminal or CommandPrompt.
- Use the following R commands.
install.packages('devtools') library(devtools) install_github('MacoskoLab/liger')
Additional Installation Steps for MacOS (recommended before step 4)
Installing RcppArmadillo on R>=3.4 requires Clang >= 4 and gfortran-6.1. Follow the instructions below if you have R version 3.4.0-3.4.4. These instructions (using clang4) may also be sufficient for R>=3.5 but for newer versions of R, it's recommended to follow the instructions in this post.
- Install gfortran as suggested here
- Download clang4 from this page
- Uncompress the resulting zip file and type into Terminal (
mv /path/to/clang4/ /usr/local/
.R/Makevarsfile containing following:
# The following statements are required to use the clang4 binary CC=/usr/local/clang4/bin/clang CXX=/usr/local/clang4/bin/clang++ CXX11=/usr/local/clang4/bin/clang++ CXX14=/usr/local/clang4/bin/clang++ CXX17=/usr/local/clang4/bin/clang++ CXX1X=/usr/local/clang4/bin/clang++ LDFLAGS=-L/usr/local/clang4/lib
For example, use the following Terminal commands:
cd ~ mkdir .R cd .R nano Makevars
Paste in the required text above and save with
Detailed Instructions for FIt-SNE Installation for use in runTSNE (recommended for large datasets)
Using FIt-SNE is recommended for computational efficiency when using runTSNE on very large datasets. Installing and compiling the necessary software requires the use of git, FIt-SNE, and FFTW. For a basic overview of installation, visit this page.
Basic installation for most Unix machines can be achieved with the following commands after downloading the latest version of FFTW from here. In the fftw directory, run:
./configure make make install
(Additional instructions if necessary). Then in desired directory:
git clone https://github.com/KlugerLab/FIt-SNE.git cd FIt-SNE g++ -std=c++11 -O3 src/sptree.cpp src/tsne.cpp src/nbodyfft.cpp -o bin/fast_tsne -pthread -lfftw3 -lm pwd
Use the output of
pwd as the
fitsne.path parameter in runTSNE.