Do you want to learn how to align RNA-sequences to a reference genome?
Do you want to bypass the process of installing alignment software and get a preview into a panel of different alignment strategies?
Do you want to compare how popular mapping algorithms perform on your data?
A Docker image is available for this project. For a detailed description of docker, please refer to this overview.
Follow instructions to install Docker for your environment.
Get a pre-built image from DockerHub and run the server
docker pull stevetsa/rna_mapping docker run -it stevetsa/rna_mapping At the root prompt inside the container, execute the script - /RNA_mapping/build/doAll.sh
Run this image on the NIH biowulf cluster
sinteractive --cpus-per-task=16 --mem=10g module load singularity singularity shell docker://stevetsa/rna_mapping inside Singularity container, execute the script - /RNA_mapping/build/doAll.sh