Overview Website: NeuBtracker.org
Paper: DOI 10.1038/NMETH.4459
| Description | Company | Part Name / ID | |
|---|---|---|---|
| Illumination | IR - LED | Roithner Laser Technik | 12x ELD-1060-525 |
| IR - LED Holder | Custom Made | Ring for LED placement | |
| GCaMP-Exc. | Mountain Photonics | UHP-T-LED-460 Ultra High Power Blue LED Light Source | |
| Obj. | 1x Objective | Nikon | HR Plan Apo WD 54 |
| 1x Objective | Nikon | CFI P-Achromat UW 1x/ 0.04/ 3,20 | |
| Tube Lens | Thorlabs | ITL200 Infinity-Corrected Tube Lens | |
| 1x/IR | IR Camera | Ximea | MQ013RG-E2 |
| 1:3 Relay | Thorlabs | 1:3.33 Matched Achromatic Pair, B Coated | |
| 1:5 Relay | Thorlabs | 150 & 50 mm Achrom. Doublet pairs | |
| 3D Scanning System | Galvo (XY) | Thorlabs | GVS312/M - 2D Large Beam (10 mm) Diameter Galvo System |
| ETL (Z) | Optotune | Fast electrically tunable lens EL-10-30-C-VIS-LD | |
| Scanning Relay and Magnification | 1 Telesc. Lens | Thorlabs | 2x 100mm, 60mm Achrom. (Plössl Configuration) |
| 2 Telesc. Lens | Thorlabs | f=150 mm, Ø2" Achromatic Doublet | |
| Zooming System | Leica | Z16 Apo | |
| 1 Telesc. Lens | Thorlabs | f=50 mm, Ø1" Achromatic Doublet, | |
| 2 Telesc. Lens | Thorlabs | f=200 mm, Ø2" Achromatic Doublet | |
| Fluor. Acq. | Camera | Andor | Zyla5.5-USB3 (or Neo sCMOS) |
| Manipulation Laser | Laser | Coherent | 407 nm / 50 mW |
| Alignment Mirrors | Thorlabs | Kinematic Mirror Mount for Ø2" Optics | |
| Coupling Lens | Thorlabs | f=200 mm, Ø2" Achromatic Doublet | |
| Controllers | Main (PC) | Terra | i7-3770, 32GB RAM, Scorpio PCI-SSD@ 2GB/s |
| for Galvo Mirrors | National Instruments | USB-9263, 4-Channel, ±10 V, 16-Bit Analog Voltage Output Module | |
| for Green LED | Arduino | Uno | |
| Filters | Filters/Dichroics | Chroma Technology | ET470/40x, T495lpxr, (LED coupling) T770lpxr, ET780lp, (IR Camera) zt405rdc (stimulation laser coupling) ET525/50m (Fluorescence Camera) |
| Filter Cubes | Thorlabs | 30 mm Cage-Compatible Fluorescence Filter Cube | |
| Example pieces for mechanical support | Rails | Thorlabs | Extended Dovetail Rail Carrier |
| Bases | Thorlabs | Standard Ø1/2" Post Holders | |
| Post | Thorlabs | Ø12.7 mm Graduated Optical Post | |
| Rods | Thorlabs | ER Assembly Rods | |
| Cage Syst. Holder | Thorlabs | 30 mm to 60 mm Cage Plate Adapter, M4 Tap | |
| Sliding Mount | Thorlabs | Removable Filter Holder | |
| Pivoting Mount | Thorlabs | Pivoting, Quick-Release, Ø1" Optic Mount | |
| Kin. Lens Mounts | Thorlabs | XY Translating Lens Mount | |
| Add-ons | Motorized Stage | Zaber | Motorized linear slide, 150 mm travel, RS-232 |
| Alternative GCaMP Excitation | Coherent | OBIS 488 nm- LX 150 mW (coupled through ZT488dcrb) | |
| 500FPS camera | Ximea | MQ003MG-CM |
Please cite our original publication in Nature Methods:
Symvoulidis P. et al. (2017) NeuBtracker – imaging neurobehavioral dynamics in freely bahaving fish. In: Nature Methods DOI: 10.1038/NMETH.4459
@Article{Symvoulidis2017,
author={Symvoulidis, Panagiotis
and Lauri, Antonella
and Stefanoiu, Anca
and Cappetta, Michele
and Schneider, Steffen
and Jia, Hongbo
and Stelzl, Anja
and Koch, Maximilian
and Perez, Carlos Cruz
and Myklatun, Ahne
and Renninger, Sabine
and Chmyrov, Andriy
and Lasser, Tobias
and Wurst, Wolfgang
and Ntziachristos, Vasilis
and Westmeyer, Gil G.},
title={NeuBtracker[mdash]imaging neurobehavioral dynamics in freely behaving fish},
journal={Nat Meth},
year={2017},
month={Oct},
day={02},
publisher={Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.},
volume={advance online publication},
note={Brief Communication},
issn={1548-7105},
url={http://dx.doi.org/10.1038/nmeth.4459}
}
Corresponding Author: Gil G Westmeyer