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How to convert metabolic parameters from literature? #271

regulus92 opened this Issue Jan 16, 2019 · 2 comments


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regulus92 commented Jan 16, 2019

Hello, I'm a beginner to PBPK and I have a question. In PKSim, some drug metabolic and transport processes under Michaelis-Menten type use units of volume such as μmol/l/min for Vmax, however, I found that mg-1 protein are more frequently used in references. For example, Vmax value of metformin transport by hOCT2-expressing HEK293 cells was 11.9 ± 1.5 nmol mg protein−1 min−1 (Naoko Kimura 2005). So how should I convert such value for PKSim modelling?


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StephanSchaller commented Jan 17, 2019

Just select one of the lower entries:



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regulus92 commented Jan 17, 2019

MW of hOCT2 is 62.59 kDa, 1 mg protein = 10e-3/62590*10e9=15.98 pmol protein, so Vmax 11.9 noml/min/mg = 0.745 nmol/min/pmol. Is this right ?

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