hERG assay - appeal for collaborator #192

mattodd opened this Issue May 2, 2014 · 2 comments


None yet

1 participant

OpenSourceMalaria member

A hERG assay was run on two compounds:

march 2014 herg compounds

Data: http://malaria.ourexperiment.org/biological_data/9562

which were both positive (numbers were pIC50 = 5.6 and 5.2 while ideally we'd want compounds to be at about 4.5). This represents a warning flag, rather than a show-stopper - hERG is typically more of a problem with bases. Reducing this activity should be an aim of future analog design in OSM's Series 4 (http://openwetware.org/wiki/OpenSourceMalaria:Triazolopyrazine_%28TP%29_Series).

Future use of this assay: The assay employed in this case was a low-throughput patch-clamp assay. It would be useful to run a higher-throughput competitive binding assay for future analogs in OSM Series 4.

Appeal - Is there a lab willing to run a high throughput hERG competitive binding assay on future Series 4 compounds? Comments can be below, or via email to opensourcemalaria@gmail.com, or via any other means of contacting members of the consortium listed on the landing page: http://opensourcemalaria.org/

@mattodd mattodd self-assigned this May 2, 2014
OpenSourceMalaria member

More data should be coming #211
But still need collaborator lab long-term here.

OpenSourceMalaria member

AstraZeneca ran the latest hERG assay
The data obtained point to an ongoing need for screening of future compounds in this assay, as well as the need for a predictive model.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment