Table of software for the analysis of single-cell RNA-seq data.
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README.md

scRNA-tools

scRNA-tools

Project Status Lifecycle

A database of software tools for the analysis of single-cell RNA-seq data. To make it into the database software must be available for download and public use somewhere (CRAN, Bioconductor, PyPI, Conda, GitHub, Bitbucket, a private website etc). To view the database head to https://www.scRNA-tools.org.

Purpose

This database is designed to be an overview of the currently available scRNA-seq analysis software, it is unlikely to be 100% complete or accurate but will be updated as new software becomes available. If you notice a problem or would like to add something please make a pull request or open an issue.

Citation

If you find the scRNA-tools database useful for your work please cite our preprint: Zappia L, Phipson B, Oshlack A. "Exploring the single-cell RNA-seq analysis landscape with the scRNA-tools database", PLOS Computational Biology (2018), DOI: 10.1371/journal.pcbi.1006245"

Structure

The main tools table has the following columns:

  • Name
  • Platform - Programming language or platform where it can be used
  • DOIs - Publication DOIs separated by semi-colons
  • PubDates - Publication dates separated with semi-colons. Preprints are marked with PREPRINT and will be updated when published.
  • Code - URL for publicly available code.
  • Description
  • License - Software license
  • FUNCTION COLUMNS (Described below)
  • Added - Date when the entry added.
  • Updated - Date when the entry was last updated.

Function columns

The function columns are TRUE/FALSE columns indicating if the software has a particular function. These are designed to be used as filters, for example when looking for software to accomplish a particular task. They are also the most likely to be inaccurate as software is frequently updated and it is hard to judge all the functions a package has without making significant use of it. The function columns ask the following questions of the software:

  • Assembly - Can it perform assembly?
  • Alignment - Does it align reads to a reference?
  • UMIs - Does it handle Unique Molecular Identifiers?
  • Quantification - Does it quantify expression from reads?
  • QualityControl - Does it perform some type of quality control of cells?
  • Normalisation - Does it perform some type of normalisation?
  • Imputation - Can it impute missing dropout values?
  • Integration - Does it combine scRNA-seq datasets or other single-cell data types?
  • GeneFiltering - Does it perform some filtering of genes?
  • Clustering - Does it perform clustering of cells?
  • Classification - Does it classify cells based on a reference dataset?
  • Ordering - Does it order cells along a (pseudotime) trajectory?
  • DifferentialExpression - Does it do some kind of differential expression?
  • MarkerGenes - Does it identify or mark use of cell type markers?
  • ExpressionPatterns - Can it find genes with interesting patterns over (psuedo) time?
  • VariableGenes - Does it identify highly variable genes?
  • GeneSets - Does it test or make use of annotated gene sets?
  • GeneNetworks - Does it find co-regulated gene networks?
  • CellCycle - Does it identify or correct for the cell cycle or cell cycle (or similar) genes?
  • DimensionalityReduction - Can it perform some type of dimensionality reduction?
  • Transformation - Does it transform between expression values and some over measure?
  • Modality - Does it identify or make use of modality in expression?
  • AlternativeSplicing - Does it identify alternatice splicing?
  • RareCells - Does it identify rare cells types?
  • StemCells - Does it identify stem cells in a population?
  • Variants - Does it detect or make use of variants?
  • Haplotypes - Does it make use of haplotypes or perform phasing?
  • AlleleSpecific - Does it detect allele specific expression?
  • Visualisation - Does it do some kind of visualisation? (showing how to make a plot using ggplot or matplotlib doesn't count)
  • Interactive - Does it have some kind of interactive component or a GUI?
  • Simulation - Does it have functions for simulating scRNA-seq data?

Contributors

Thank you to everyone who has contributed to scRNA-tools! Your efforts to build and improve this resource for the community are greatly appreciated!

The following people have made significant contributions to the scRNA-tools database or website:

These people have added new tools or updated existing entries in the database:

@nsalomonis, @TomSmithCGAT, @PeteHaitch, @lucapinello