LCMS raw data and MSGF+ results viewer
LCMS-Spectator is a standalone Windows program for viewing LC-MS data and peptide identifications.
It reads Thermo raw files (*.raw) and mzML files and shows MS and MS/MS spectra as well as precursor and product ion chromatograms as resizable, floatable tabbed-documents.
Identification and target files can also be loaded to show the spectral and chromatographic evidence. The tool currently supports MS-GF+ MzIdentML files (.mzid) and MSPathFinder tab-delimited result files (.tsv).
It uses Informed-Proteomics for reading spectrum files and can function as a GUI for ProMex and MSPathFinder.
A guide for using LCMS-Spectator can be found on our GitHub Wiki Page.
The latest version of the application is available on the AppVeyor CI server
Supported file formats
LCMS-Spectator supports the use of centroided mzML files as spectrum input. It can also directly read Thermo .Raw files using the bundled Thermo RawFileReader library.
Several other formats are supported if an appropriate version of ProteoWizard is installed (Download here; make sure the version downloaded matches system architecture, typically Windows 64-bit)
Search result files
Supports .mzid files, but will only show limited information about the results for .mzid files that were not produced by MS-GF+ or MSPathFinder (scores will not be shown for other search tools).
Also supports .tsv files created by MSPathFinder or MS-GF+.
- .NET 4.6.2
- 2.4 GHz, quad-core CPU
- 16 GB RAM
- Windows 7 or newer
- 250 GB hard drive
Running MSPathFinder from LCMS Spectator
The suggested method for running MSPathFinder is to use the console version, available at https://github.com/PNNL-Comp-Mass-Spec/Informed-Proteomics/releases This has the advantage that you can see all of the log messages shown at the console.
However, LCMS Spectator also supports running MSPathFinder, as outlined here:
Convert your data file to a centroided .mzML file
msconvert.exe --32 --mzML --filter "peakPicking true 1-" DatasetName.raw
Start LCMS Spectator and choose the Search menu
Define the Dataset Info
- Spectrum File Path: path to the .mzML file
- Fasta DB File Path: path to the protein sequence FASTA file
- Feature File path: leave blank
- Output Directory: will be auto-defined as the directory with the .mzML file, but can be changed
- Define the search settings
- Default settings are shown; customize if desired
- If you specify a custom scan range, the features file (.ms1ft) will not be created, and the viewing of MS/MS spectra will show all data points, not just peaks, which can greatly slow down the viewer
- Select the proteins to search
- By default, selects all proteins in the FASTA file
Define dynamic and/or static modifications
- A progress dialog will appear, but will stay blank while the .mzML file is being processed to create the .pbf file
- Next, a new FASTA file is created based on the specified FASTA file
- It will include the proteins you seleced on the Sequence tab
- It tracks the protein name and the first word of the protein description, plus the residues of each protein
The FASTA file will be indexed, creating files .icanno, .icseq, and .icplcp
The target (forward) sequence database will be searched
The decoy (shuffled) sequence database will be searched
When finished, E-values are computed and results written, creating a .tsv file named DatasetName_IcDecoy.tsv
Written by Chris Wilkins and Bryson Gibbons for the Department of Energy (PNNL, Richland, WA)
E-mail: firstname.lastname@example.org or email@example.com
Website: https://omics.pnl.gov/ or https://panomics.pnnl.gov/
Licensed under the Educational Community License, Version 2.0 (the "License"); you may not use this file except in compliance with the License. You may obtain a copy of the License at http://www.osedu.org/licenses/ECL-2.0
Unless required by applicable law or agreed to in writing, software distributed under the License is distributed on an "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the License for the specific language governing permissions and limitations under the License.
Copyright 2018 Battelle Memorial Institute
RawFileReader reading tool. Copyright © 2016 by Thermo Fisher Scientific, Inc. All rights reserved.