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Welcome to the pbalign wiki!
ATTENTION: pbalign and blasr has been significantly changed in SMRTAnalysis v3.0. And blasr and pbalign in SMRTAnalysis v3.0 (and later version) are NOT BACKWARD-COMPATIBLE with legacy blasr and pbalign in SMRTAnalysis v2.3 (and previous version).
BAM files and represented by DATASETS. We recommend to use SMRTAnalysis v3.0+ (e.g., the latest blasr and pbalign in GitHub) to align SEQUEL sequencing data.PacBio SEQUEL sequencing data are now saved in
convert BAX/BAS.H5 files to BAM files and then analyze BAM files using SMRTAnalysis v3.0+ (e.g., the latest blasr and pbalign in GitHub)PacBio RSII sequencing data used to be saved in BAX/BAS.H5 files. Although users can still use SMRTAnalysis v2.3- for analyzing RSII sequencing data, we recommend to first
pbalign v0.3.0 no longer supports CMP.H5 output, and must go with blasr v5.1+.
pbalign v0.2.0 replaced
[SMRTAnalysis v3.0+: Deal with large RSII and SEQUEL datasets using the latest pbalign/blasr?] --- TBD