A toolkit for working with ATAC-seq data.
What's in the box?
Programs we've found useful in ATAC-seq pipelines
trim_adapters: based on Jason Buenrostro's utility for trimming Illumina adapters by aligning paired reads to each other.
make_cut_matrix: useful in conjunction with CENTIPEDE, and in generating plots of transcription factor binding sites.
plot_aggregate_matrix.R: generates plots for motifs given the aggregate output of make_cut_matrix
A Python library you can use in your own tools for processing ATAC-seq data
The code underpinning our command-line tools has allowed us to make our pipelines shorter and faster. Our ATAC-seq scoring functions work directly with a BAM file and run in parallel, without the overhead of invoking external applications. Particularly if you're trying to produce quantitative metrics from your data, starting with your BAM files, converting them to BED and bigWig so you can run bigWigSummary, you might find your pipeline can be simplified too.
- Python. We've run it successfully under versions 2.7.10 and 3.4.3.
At the command line:
git clone https://github.com/ParkerLab/atactk pip install ./atactk
GPLv3 or any later version.