diff --git a/src/prefect/server/schemas/actions.py b/src/prefect/server/schemas/actions.py
index e6673797de00..8cfce54611ba 100644
--- a/src/prefect/server/schemas/actions.py
+++ b/src/prefect/server/schemas/actions.py
@@ -434,7 +434,6 @@ class FlowRunCreate(ActionBaseModel):
         ),
         deprecated=True,
     )
-    job_variables: Optional[Dict[str, Any]] = FieldFrom(schemas.core.FlowRun)
 
     class Config(ActionBaseModel.Config):
         json_dumps = orjson_dumps_extra_compatible
diff --git a/tests/server/api/test_flow_runs.py b/tests/server/api/test_flow_runs.py
index 198bb2288e0c..c7148ff73819 100644
--- a/tests/server/api/test_flow_runs.py
+++ b/tests/server/api/test_flow_runs.py
@@ -49,24 +49,6 @@ async def test_create_flow_run(self, flow, client, session):
         )
         assert flow_run.flow_id == flow.id
 
-    async def test_create_flow_run_with_job_variables(self, flow, client, session):
-        job_vars = {"key": "value"}
-        response = await client.post(
-            "/flow_runs/",
-            json=actions.FlowRunCreate(
-                flow_id=flow.id,
-                name="",
-                job_variables=job_vars,
-            ).dict(json_compatible=True),
-        )
-        assert response.status_code == status.HTTP_201_CREATED
-        assert response.json()["job_variables"] == job_vars
-
-        flow_run = await models.flow_runs.read_flow_run(
-            session=session, flow_run_id=response.json()["id"]
-        )
-        assert flow_run.job_variables == job_vars
-
     async def test_create_flow_run_with_infrastructure_document_id(
         self, flow, client, infrastructure_document_id
     ):
diff --git a/tests/server/models/test_flow_runs.py b/tests/server/models/test_flow_runs.py
index 503460a34e61..50b3048bf454 100644
--- a/tests/server/models/test_flow_runs.py
+++ b/tests/server/models/test_flow_runs.py
@@ -17,18 +17,6 @@ async def test_create_flow_run(self, flow, session):
         )
         assert flow_run.flow_id == flow.id
 
-    @pytest.mark.parametrize("job_vars", [{}, None, {"foo": "bar"}])
-    async def test_create_flow_run_with_job_variables(
-        self, flow, session, job_vars: dict
-    ):
-        flow_run = await models.flow_runs.create_flow_run(
-            session=session,
-            flow_run=schemas.core.FlowRun(flow_id=flow.id, job_variables=job_vars),
-        )
-
-        expected = {} if job_vars is None else job_vars
-        assert flow_run.job_variables == expected
-
     async def test_create_flow_run_with_infrastructure(
         self, flow, session, infrastructure_document_id
     ):