diff --git a/benches/conftest.py b/benches/conftest.py
index 386e4ce98723..a8f8313c0128 100644
--- a/benches/conftest.py
+++ b/benches/conftest.py
@@ -20,14 +20,3 @@ def handle_saving(*args, **kwargs):
 
 
 pytest_benchmark.session.BenchmarkSession.handle_saving = handle_saving
-
-
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
diff --git a/src/integrations/prefect-aws/tests/conftest.py b/src/integrations/prefect-aws/tests/conftest.py
index f6a9132ff519..c53607460b61 100644
--- a/src/integrations/prefect-aws/tests/conftest.py
+++ b/src/integrations/prefect-aws/tests/conftest.py
@@ -44,14 +44,3 @@ def aws_client_parameters_empty():
 @pytest.fixture
 def aws_client_parameters_public_bucket():
     return AwsClientParameters(config=Config(signature_version=UNSIGNED))
-
-
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
diff --git a/src/integrations/prefect-azure/tests/conftest.py b/src/integrations/prefect-azure/tests/conftest.py
index 4cb3d9f0a38d..21f8fbf8eea9 100644
--- a/src/integrations/prefect-azure/tests/conftest.py
+++ b/src/integrations/prefect-azure/tests/conftest.py
@@ -17,17 +17,6 @@ def prefect_db():
         yield
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 class AsyncIter:
     def __init__(self, items):
         self.items = items
diff --git a/src/integrations/prefect-bitbucket/tests/conftest.py b/src/integrations/prefect-bitbucket/tests/conftest.py
index 8f7797306f5d..a06c90e85232 100644
--- a/src/integrations/prefect-bitbucket/tests/conftest.py
+++ b/src/integrations/prefect-bitbucket/tests/conftest.py
@@ -10,14 +10,3 @@ def prefect_db():
     """
     with prefect_test_harness():
         yield
-
-
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
diff --git a/src/integrations/prefect-dask/tests/conftest.py b/src/integrations/prefect-dask/tests/conftest.py
index c95d93952428..7292781b3329 100644
--- a/src/integrations/prefect-dask/tests/conftest.py
+++ b/src/integrations/prefect-dask/tests/conftest.py
@@ -17,17 +17,6 @@ def prefect_db():
         yield
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 @pytest.fixture(scope="session")
 def event_loop(request):
     """
diff --git a/src/integrations/prefect-databricks/tests/conftest.py b/src/integrations/prefect-databricks/tests/conftest.py
index 8f7797306f5d..a06c90e85232 100644
--- a/src/integrations/prefect-databricks/tests/conftest.py
+++ b/src/integrations/prefect-databricks/tests/conftest.py
@@ -10,14 +10,3 @@ def prefect_db():
     """
     with prefect_test_harness():
         yield
-
-
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
diff --git a/src/integrations/prefect-dbt/tests/conftest.py b/src/integrations/prefect-dbt/tests/conftest.py
index af1df7b14299..64263c1b7b43 100644
--- a/src/integrations/prefect-dbt/tests/conftest.py
+++ b/src/integrations/prefect-dbt/tests/conftest.py
@@ -36,17 +36,6 @@ def prefect_db():
         yield
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 @pytest.fixture(autouse=True)
 def google_auth_mock(monkeypatch):
     """
diff --git a/src/integrations/prefect-docker/tests/conftest.py b/src/integrations/prefect-docker/tests/conftest.py
index b1b41748ef6b..de600cde9c4a 100644
--- a/src/integrations/prefect-docker/tests/conftest.py
+++ b/src/integrations/prefect-docker/tests/conftest.py
@@ -32,17 +32,6 @@ def prefect_db():
         yield
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 @pytest.fixture(scope="session")
 def event_loop(request):
     """
diff --git a/src/integrations/prefect-gcp/tests/conftest.py b/src/integrations/prefect-gcp/tests/conftest.py
index d20a8754c7e6..1b46093ecfd7 100644
--- a/src/integrations/prefect-gcp/tests/conftest.py
+++ b/src/integrations/prefect-gcp/tests/conftest.py
@@ -24,17 +24,6 @@ def disable_logging():
         yield
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 @pytest.fixture
 def google_auth(monkeypatch):
     google_auth_mock = MagicMock()
diff --git a/src/integrations/prefect-github/tests/conftest.py b/src/integrations/prefect-github/tests/conftest.py
index 8f7797306f5d..a06c90e85232 100644
--- a/src/integrations/prefect-github/tests/conftest.py
+++ b/src/integrations/prefect-github/tests/conftest.py
@@ -10,14 +10,3 @@ def prefect_db():
     """
     with prefect_test_harness():
         yield
-
-
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
diff --git a/src/integrations/prefect-gitlab/tests/conftest.py b/src/integrations/prefect-gitlab/tests/conftest.py
index 8f7797306f5d..a06c90e85232 100644
--- a/src/integrations/prefect-gitlab/tests/conftest.py
+++ b/src/integrations/prefect-gitlab/tests/conftest.py
@@ -10,14 +10,3 @@ def prefect_db():
     """
     with prefect_test_harness():
         yield
-
-
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
diff --git a/src/integrations/prefect-kubernetes/tests/conftest.py b/src/integrations/prefect-kubernetes/tests/conftest.py
index 1cec19320f58..477440141f88 100644
--- a/src/integrations/prefect-kubernetes/tests/conftest.py
+++ b/src/integrations/prefect-kubernetes/tests/conftest.py
@@ -34,17 +34,6 @@ def disable_api_logging():
         yield
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 @pytest.fixture
 def kube_config_dict():
     return yaml.safe_load(GOOD_CONFIG_FILE_PATH.read_text())
diff --git a/src/integrations/prefect-snowflake/tests/conftest.py b/src/integrations/prefect-snowflake/tests/conftest.py
index bcb517a19554..1cf1f6d78aa3 100644
--- a/src/integrations/prefect-snowflake/tests/conftest.py
+++ b/src/integrations/prefect-snowflake/tests/conftest.py
@@ -35,17 +35,6 @@ def prefect_db():
             raise e
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 @pytest.fixture()
 def credentials_params():
     return {
diff --git a/src/integrations/prefect-sqlalchemy/tests/conftest.py b/src/integrations/prefect-sqlalchemy/tests/conftest.py
index 69455a2fde59..70ecf5f1c256 100644
--- a/src/integrations/prefect-sqlalchemy/tests/conftest.py
+++ b/src/integrations/prefect-sqlalchemy/tests/conftest.py
@@ -13,17 +13,6 @@ def prefect_db():
         yield
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 @pytest.fixture(autouse=True)
 def fetch_state_result():
     with temporary_settings(updates={PREFECT_ASYNC_FETCH_STATE_RESULT: True}):
diff --git a/src/prefect/flows.py b/src/prefect/flows.py
index d48ac84caf6c..eb8670ec512d 100644
--- a/src/prefect/flows.py
+++ b/src/prefect/flows.py
@@ -56,7 +56,7 @@
 from prefect.client.schemas.objects import FlowRun
 from prefect.client.schemas.schedules import SCHEDULE_TYPES
 from prefect.client.utilities import client_injector
-from prefect.context import PrefectObjectRegistry, registry_from_script
+from prefect.context import registry_from_script
 from prefect.deployments.runner import deploy
 from prefect.deployments.steps.core import run_steps
 from prefect.docker.docker_image import DockerImage
@@ -125,7 +125,6 @@
     from prefect.flows import FlowRun
 
 
-@PrefectObjectRegistry.register_instances
 class Flow(Generic[P, R]):
     """
     A Prefect workflow definition.
@@ -1699,29 +1698,26 @@ def load_flow_from_entrypoint(
         FlowScriptError: If an exception is encountered while running the script
         MissingFlowError: If the flow function specified in the entrypoint does not exist
     """
-    with PrefectObjectRegistry(  # type: ignore
-        block_code_execution=True,
-        capture_failures=True,
-    ):
-        if ":" in entrypoint:
-            # split by the last colon once to handle Windows paths with drive letters i.e C:\path\to\file.py:do_stuff
-            path, func_name = entrypoint.rsplit(":", maxsplit=1)
-        else:
-            path, func_name = entrypoint.rsplit(".", maxsplit=1)
-        try:
-            flow = import_object(entrypoint)
-        except AttributeError as exc:
-            raise MissingFlowError(
-                f"Flow function with name {func_name!r} not found in {path!r}. "
-            ) from exc
 
-        if not isinstance(flow, Flow):
-            raise MissingFlowError(
-                f"Function with name {func_name!r} is not a flow. Make sure that it is "
-                "decorated with '@flow'."
-            )
+    if ":" in entrypoint:
+        # split by the last colon once to handle Windows paths with drive letters i.e C:\path\to\file.py:do_stuff
+        path, func_name = entrypoint.rsplit(":", maxsplit=1)
+    else:
+        path, func_name = entrypoint.rsplit(".", maxsplit=1)
+    try:
+        flow = import_object(entrypoint)
+    except AttributeError as exc:
+        raise MissingFlowError(
+            f"Flow function with name {func_name!r} not found in {path!r}. "
+        ) from exc
 
-        return flow
+    if not isinstance(flow, Flow):
+        raise MissingFlowError(
+            f"Function with name {func_name!r} is not a flow. Make sure that it is "
+            "decorated with '@flow'."
+        )
+
+    return flow
 
 
 def load_flow_from_text(script_contents: AnyStr, flow_name: str) -> Flow:
diff --git a/src/prefect/runner/server.py b/src/prefect/runner/server.py
index 70376c473a0a..018558067a6c 100644
--- a/src/prefect/runner/server.py
+++ b/src/prefect/runner/server.py
@@ -10,7 +10,7 @@
 from prefect._internal.schemas.validators import validate_values_conform_to_schema
 from prefect.client.orchestration import get_client
 from prefect.exceptions import MissingFlowError, ScriptError
-from prefect.flows import Flow, load_flow_from_entrypoint, load_flows_from_script
+from prefect.flows import Flow, load_flow_from_entrypoint
 from prefect.logging import get_logger
 from prefect.runner.utils import (
     inject_schemas_into_openapi,
@@ -24,6 +24,7 @@
     PREFECT_RUNNER_SERVER_PORT,
 )
 from prefect.utilities.asyncutils import sync_compatible
+from prefect.utilities.importtools import load_script_as_module
 
 if TYPE_CHECKING:
     from prefect.client.schemas.responses import DeploymentResponse
@@ -155,7 +156,10 @@ async def get_subflow_schemas(runner: "Runner") -> Dict[str, Dict]:
                 continue
 
             script = deployment.entrypoint.split(":")[0]
-            subflows = load_flows_from_script(script)
+            module = load_script_as_module(script)
+            subflows = [
+                obj for obj in module.__dict__.values() if isinstance(obj, Flow)
+            ]
             for flow in subflows:
                 schemas[flow.name] = flow.parameters.model_dump()
 
diff --git a/src/prefect/tasks.py b/src/prefect/tasks.py
index d2387db025a6..a5f5fd65e3a1 100644
--- a/src/prefect/tasks.py
+++ b/src/prefect/tasks.py
@@ -37,7 +37,6 @@
 from prefect.client.schemas.objects import TaskRunInput, TaskRunResult
 from prefect.context import (
     FlowRunContext,
-    PrefectObjectRegistry,
     TagsContext,
     TaskRunContext,
     serialize_context,
@@ -174,7 +173,6 @@ def _infer_parent_task_runs(
     return parents
 
 
-@PrefectObjectRegistry.register_instances
 class Task(Generic[P, R]):
     """
     A Prefect task definition.
diff --git a/tests/conftest.py b/tests/conftest.py
index adc3ceefcf79..f188692c2322 100644
--- a/tests/conftest.py
+++ b/tests/conftest.py
@@ -482,17 +482,6 @@ def test_database_connection_url(generate_test_database_connection_url):
             yield url
 
 
-@pytest.fixture(autouse=True)
-def reset_object_registry():
-    """
-    Ensures each test has a clean object registry.
-    """
-    from prefect.context import PrefectObjectRegistry
-
-    with PrefectObjectRegistry():
-        yield
-
-
 @pytest.fixture(autouse=True)
 def reset_registered_blocks():
     """
diff --git a/tests/test_flows.py b/tests/test_flows.py
index 82f4503d18b6..3895445f878e 100644
--- a/tests/test_flows.py
+++ b/tests/test_flows.py
@@ -31,7 +31,6 @@
     IntervalSchedule,
     RRuleSchedule,
 )
-from prefect.context import PrefectObjectRegistry
 from prefect.deployments.runner import RunnerDeployment
 from prefect.docker.docker_image import DockerImage
 from prefect.events import DeploymentEventTrigger, Posture
@@ -743,16 +742,6 @@ def foo():
         with pytest.raises(ValueError, match="Test 2"):
             await second.result()
 
-    @pytest.mark.skip(reason="Fails with new engine, passed on old engine")
-    async def test_call_execution_blocked_does_not_run_flow(self):
-        @flow(version="test")
-        def foo(x, y=3, z=3):
-            return x + y + z
-
-        with PrefectObjectRegistry(block_code_execution=True):
-            state = foo(1, 2)
-            assert state is None
-
     def test_flow_can_end_in_paused_state(self):
         @flow
         def my_flow():