Skip to content
Merged
Show file tree
Hide file tree
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
324 changes: 324 additions & 0 deletions docs/user_guide/02_analytical/plate-reading/bmg-clariostar.ipynb
Original file line number Diff line number Diff line change
@@ -0,0 +1,324 @@
{
"cells": [
{
"cell_type": "markdown",
"id": "18708b66",
"metadata": {},
"source": [
"# BMG Labtech CLARIOstar (Plus)\n",
"\n",
"| Summary | Photo |\n",
"|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|----------------------------------------------------|\n",
"| - [OEM Link](https://www.bmglabtech.com/en/clariostar-plus/)<br>- **Communication Protocol / Hardware**: Serial (FTDI)/ USB-A<br>- **Communication Level**: Firmware<br>- **Measurement Modes**: Absorbance, Luminescence, Fluorescence <br>- **Plate Delivery**: Loading tray<br>- **Additional Standard Features**: Temperature sontrol, Shaking<br><br>- **Additional Upgrades**: Injector system, increased max temperature, plate stacking system, ... | ![quadrants](img/bmg-labtech-clariostar-plus.png) |\n"
]
},
{
"cell_type": "markdown",
"id": "80e2e5dc",
"metadata": {},
"source": [
"---\n",
"## Setup Instructions (Physical)\n",
"\n",
"The CLARIOstar and CLARIOstar Plus require a minimum of two cable connections to be operational:\n",
"1. Power cord (standard IEC C13)\n",
"2. USB cable (USB-B with security screws at CLARIOstar end; USB-A at control PC end)\n",
"\n",
"Optional:\n",
"If you have a plate stacking unit to use with the CLARIOstar (Plus), an additional RS-232 port is available on the CLARIOstar (Plus).\n"
]
},
{
"cell_type": "markdown",
"id": "adb29364",
"metadata": {},
"source": [
"---\n",
"## Setup Instructions (Programmatic)\n",
"\n"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "34531f2c",
"metadata": {},
"outputs": [],
"source": [
"%load_ext autoreload\n",
"%autoreload 2"
]
},
{
"cell_type": "markdown",
"id": "e7a179e9",
"metadata": {},
"source": [
"To control the BMG Labtech CLARIOstar (Plus), generate a `PlateReader` frontend instance that uses a `CLARIOstarBackend` instance as its backend.\n",
"\n",
"To access the CLARIOstar-specific machine features you can still use the backend directly.\n",
"For convenience, it is useful to therefore store the backend instance as a separate `clariostar_backend` variable."
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "363b8144",
"metadata": {},
"outputs": [],
"source": [
"from pylabrobot.plate_reading import PlateReader\n",
"\n",
"from pylabrobot.plate_reading.clario_star_backend import CLARIOstarBackend\n",
"clariostar_backend = CLARIOstarBackend()\n",
"\n",
"pr = PlateReader(\n",
" name=\"CLARIOstar\",\n",
" backend=clariostar_backend,\n",
" size_x=0.0, # TODO: generate new handling for resources with loading tray \n",
" size_y=0.0,\n",
" size_z=0.0\n",
" )"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "30720acb",
"metadata": {},
"outputs": [],
"source": [
"await pr.setup()"
]
},
{
"cell_type": "markdown",
"id": "65555028",
"metadata": {},
"source": [
"```{note}\n",
"Expected behaviour: the machine should perform its initialization routine.\n",
"```"
]
},
{
"cell_type": "markdown",
"id": "7d2e9ed2",
"metadata": {},
"source": [
"---\n",
"\n",
"## Usage / Machine Features\n",
"\n",
"### Loading Tray"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "c0834a6e",
"metadata": {},
"outputs": [],
"source": [
"await pr.open()"
]
},
{
"cell_type": "code",
"execution_count": 2,
"id": "77c3406b",
"metadata": {},
"outputs": [],
"source": [
"# perform arm movement to move your plate of interest onto the CLARIOstar's loading tray\n",
"# this movement can be performed by a human\n",
"# or it can be performed by a robotic arm"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "092a02fa",
"metadata": {},
"outputs": [],
"source": [
"await pr.close()"
]
},
{
"cell_type": "markdown",
"id": "9c4d21b7",
"metadata": {},
"source": [
"### Set Temperature\n",
"\n",
"The CLARIOstar offers a temperature control feature.\n",
"Reaching a set temperature is relatively slow compared to standalone temperature controllers.\n",
"We therefore recommend setting the temperature early on in your automated Protocol (aP)."
]
},
{
"cell_type": "code",
"execution_count": 3,
"id": "bb2de16b",
"metadata": {},
"outputs": [],
"source": [
"# WIP: feature exposure in active development"
]
},
{
"cell_type": "markdown",
"id": "bf4840ea",
"metadata": {},
"source": [
"### Set Shaking\n",
"\n",
"The CLARIOstar offers a shaking feature."
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "60b71bea",
"metadata": {},
"outputs": [],
"source": [
"# WIP: feature in active development"
]
},
{
"cell_type": "markdown",
"id": "c4be6218",
"metadata": {},
"source": [
"---\n",
"### Measuring Absorbance\n",
"\n"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "e23acc3d",
"metadata": {},
"outputs": [],
"source": [
"# WIP: feature in active development including\n",
"# reading subsets of wells\n",
"# specifying orbital diameter\n",
"# specifying number of technical replicate measurements per well\n",
"# specifying start position for reading: topleft, topright, bottomleft, bottomright\n",
"# ...\n",
"\n",
"results_absorbance = await pr.read_absorbance()\n"
]
},
{
"cell_type": "markdown",
"id": "d9a13de2",
"metadata": {},
"source": [
"`results` will be a width x height array of absorbance values.\n",
"\n"
]
},
{
"cell_type": "markdown",
"id": "e57e55de",
"metadata": {},
"source": [
"\n",
"#### Performing a Spectral Scan\n",
"\n"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "8cae50a6",
"metadata": {},
"outputs": [],
"source": [
"# WIP: feature in active development"
]
},
{
"cell_type": "markdown",
"id": "c7a70d7e",
"metadata": {},
"source": [
"### Measuring Luminescence\n"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "c8656ef6",
"metadata": {},
"outputs": [],
"source": [
"# WIP: feature in active development\n",
"\n",
"results_luminescence = await pr.read_luminescence()"
]
},
{
"cell_type": "markdown",
"id": "8554a66c",
"metadata": {},
"source": [
"### Measuring Fluorescence\n"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "637f06a7",
"metadata": {},
"outputs": [],
"source": [
"# WIP: feature in active development"
]
},
{
"cell_type": "markdown",
"id": "362dd696",
"metadata": {},
"source": [
"### Using the Injector Needles\n"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "cd676c58",
"metadata": {},
"outputs": [],
"source": [
"# WIP: feature in active development"
]
}
],
"metadata": {
"kernelspec": {
"display_name": "plr",
"language": "python",
"name": "python3"
},
"language_info": {
"codemirror_mode": {
"name": "ipython",
"version": 3
},
"file_extension": ".py",
"mimetype": "text/x-python",
"name": "python",
"nbconvert_exporter": "python",
"pygments_lexer": "ipython3",
"version": "3.12.11"
}
},
"nbformat": 4,
"nbformat_minor": 5
}
49 changes: 0 additions & 49 deletions docs/user_guide/02_analytical/plate-reading/bmg-clariostar.rst

This file was deleted.

Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Original file line number Diff line number Diff line change
Expand Up @@ -3,8 +3,9 @@ Plate Readers

PyLabRobot supports the following plate readers:

- :ref:`BMG Clariostar <clariostar>`
- `BMG Labtech CLARIOstar`

Plate readers are controlled by the :class:`~pylabrobot.plate_reading.plate_reader.PlateReader` class. This class takes a backend as an argument. The backend is responsible for communicating with the plate reader and is specific to the hardware being used.


.. toctree::
Expand Down
2 changes: 1 addition & 1 deletion docs/user_guide/machines.md
Original file line number Diff line number Diff line change
Expand Up @@ -144,7 +144,7 @@ tr > td:nth-child(5) { width: 15%; }

| Manufacturer | Machine | Features | PLR-Support | Links |
|--------------|---------|----------|-------------|--------|
| BMG Labtech | CLARIOstar | <span class="badge badge-absorbance">absorbance</span><span class="badge badge-fluorescence">fluorescence</span><span class="badge badge-luminescence">luminescence</span> | Full | [PLR](https://docs.pylabrobot.org/user_guide/02_analytical/plate-reading/bmg-clariostar.html) / [OEM](https://www.bmglabtech.com/en/clariostar-plus/) |
| BMG Labtech | CLARIOstar (Plus) | <span class="badge badge-absorbance">absorbance</span><span class="badge badge-fluorescence">fluorescence</span><span class="badge badge-luminescence">luminescence</span> | Full | [PLR](https://docs.pylabrobot.org/user_guide/02_analytical/plate-reading/bmg-clariostar.html) / [OEM](https://www.bmglabtech.com/en/clariostar-plus/) |
| Agilent (BioTek) | Cytation 1 | <span class="badge badge-absorbance">absorbance</span><span class="badge badge-fluorescence">fluorescence</span><span class="badge badge-luminescence">luminescence</span><span class="badge badge-microscopy">microscopy</span> | Full | [PLR](https://docs.pylabrobot.org/user_guide/02_analytical/plate-reading/cytation5.html) / [OEM](https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-1-cell-imaging-multimode-reader-1623200) |
| Agilent (BioTek) | Cytation 5 | <span class="badge badge-absorbance">absorbance</span><span class="badge badge-fluorescence">fluorescence</span><span class="badge badge-luminescence">luminescence</span><span class="badge badge-microscopy">microscopy</span> | Full | [PLR](https://docs.pylabrobot.org/user_guide/02_analytical/plate-reading/cytation5.html) / [OEM](https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-5-cell-imaging-multimode-reader-1623202) |
| Byonoy | Absorbance 96 Automate | <span class="badge badge-absorbance">absorbance</span> | WIP | [OEM](https://byonoy.com/absorbance-96-automate/) |
Expand Down
Loading
Loading