diff --git a/docs/resources/library/hamilton.md b/docs/resources/library/hamilton.md
index 307df97d9ed..76154017749 100644
--- a/docs/resources/library/hamilton.md
+++ b/docs/resources/library/hamilton.md
@@ -50,7 +50,7 @@ Sometimes called "sample carriers" in Hamilton jargon.
 | Description | Image | PLR definition |
 | - | - | - |
 | 'Tube_CAR_24_A00'<br>Part no.: 173400<br>[manufacturer website](https://www.hamiltoncompany.com/automated-liquid-handling/other-robotics/173400) <br>Carries 24 "sample" tubes with 14.5–18 mm outer diameter, 60–120 mm high. Occupies one track. | ![](img/hamilton/Tube_CAR_24_A00.png) | `Tube_CAR_24_A00` |
-| 'Tube_CAR_32_A00'<br>Part no.: 173410<br>[manufacturer website](https://www.hamiltoncompany.com/other-robotics/173410) <br>Carries 32 "sample" tubes with 11–14 mm outer diameter, 60–120 mm high. Occupies one track. | ![](img/hamilton/Tube_CAR_32_A00.png.avif) | `Tube_CAR_32_A00` |
+| 'hamilton_tube_carrier_32_a00_insert_eppendorf_1_5mL'<br>Part no.: 173410<br>[manufacturer website](https://www.hamiltoncompany.com/other-robotics/173410) <br>Carries 32 `Eppendorf_DNA_LoBind_1_5ml_Vb` or `Eppendorf_Protein_LoBind_1_5ml_Vb` tubes. Occupies one track. | ![](img/hamilton/Tube_CAR_32_A00.png.avif) | `hamilton_tube_carrier_32_a00_insert_eppendorf_1_5mL` |
 | 'hamilton_tube_carrier_12_b00'<br>Part no.: 182045<br>[manufacturer website](https://www.hamiltoncompany.com/other-robotics/50-ml-falcon-tube-carrier) <br>Carries 12 "sample" tubes with 30 mm outer diameter, 115 mm high. Occupies two tracks. | ![](img/hamilton/hamilton_tube_carrier_12_b00.jpg) | `hamilton_tube_carrier_12_b00` |
 
 ### Trough carriers
diff --git a/pylabrobot/resources/hamilton/tube_carriers.py b/pylabrobot/resources/hamilton/tube_carriers.py
index 7a34ebd53fd..6d6d233068e 100644
--- a/pylabrobot/resources/hamilton/tube_carriers.py
+++ b/pylabrobot/resources/hamilton/tube_carriers.py
@@ -1,3 +1,5 @@
+import warnings
+
 from pylabrobot.resources.carrier import (
   Coordinate,
   ResourceHolder,
@@ -31,25 +33,42 @@ def Tube_CAR_24_A00(name: str) -> TubeCarrier:
 
 
 def Tube_CAR_32_A00(name: str) -> TubeCarrier:
-  """Hamilton cat. no.: 173410
+  warnings.warn(
+    "The true dimensions of a Tube_CAR_32_A00 (SMP_CAR_32_A00) are not known. "
+    "The hamilton definitions are with inserts. "
+    "Do you want to use `hamilton_tube_carrier_32_a00_insert_eppendorf_1_5mL` instead?",
+    DeprecationWarning,
+  )
+  return hamilton_tube_carrier_32_a00_insert_eppendorf_1_5mL(name)
+
+
+def hamilton_tube_carrier_32_a00_insert_eppendorf_1_5mL(name: str) -> TubeCarrier:
+  """Hamilton cat. no.: 173410 with inserts cat no. 187350.
   Hamilton name: 'SMP_CAR_32_A00'.
   'Sample' carrier for 32 tubes
   1 track(T) wide.
+  For use with `Eppendorf_DNA_LoBind_1_5ml_Vb` and `Eppendorf_Protein_LoBind_1_5ml_Vb`.
   """
+  hole_diameter = 10.8
   return TubeCarrier(
     name=name,
-    size_x=22.5,
-    size_y=497.0,
-    size_z=71.5,
+    size_x=22.5,  # 1 track
+    size_y=497.0,  # standard
+    size_z=60.2,  # caliper
     sites=create_homogeneous_resources(
       klass=ResourceHolder,
-      locations=[Coordinate(5, 6.5 + x * 15, 24.0) for x in range(32)],
+      locations=[
+        Coordinate(14.5 - hole_diameter / 2, 14.5 - hole_diameter / 2 + x * 15, 25.88)
+        for x in range(
+          32
+        )  # SMP_CAR_32_A00 in venus, verified with caliper, custom z from z probing
+      ],
       # should fix container
-      resource_size_x=13.0,
-      resource_size_y=13.0,
+      resource_size_x=hole_diameter,  # venus
+      resource_size_y=hole_diameter,  # venus
       name_prefix=name,
     ),
-    model="Tube_CAR_32_A00",
+    model=hamilton_tube_carrier_32_a00_insert_eppendorf_1_5mL.__name__,
   )
 
 
@@ -59,6 +78,7 @@ def hamilton_tube_carrier_12_b00(name: str) -> TubeCarrier:
   'Sample' carrier for 12 50mL falcon tubes (Cor_Falcon_tube_50mL_Vb).
   2 track(T) wide.
   """
+  hole_diameter = 29.0
   return TubeCarrier(
     name=name,
     size_x=45,  # 2 tracks
@@ -67,11 +87,11 @@ def hamilton_tube_carrier_12_b00(name: str) -> TubeCarrier:
     sites=create_homogeneous_resources(
       klass=ResourceHolder,
       locations=[
-        Coordinate(26.05 - 29 / 2, 41.74 - 29 / 2 + i * 36.5, 18.9)
+        Coordinate(26.05 - hole_diameter / 2, 41.74 - hole_diameter / 2 + i * 36.5, 18.9)
         for i in range(12)  # SMP_CAR_12_A00 in venus, verified with caliper, custom z
       ],
-      resource_size_x=29.0,
-      resource_size_y=29.0,
+      resource_size_x=hole_diameter,
+      resource_size_y=hole_diameter,
       name_prefix=name,
     ),
     model=hamilton_tube_carrier_12_b00.__name__,