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RTG Tools

Copyright (c) 2018 Real Time Genomics Ltd

This software is provided under the Simplified BSD License. See LICENSE.txt


RTG Tools is a subset of RTG Core that includes several useful utilities for dealing with VCF files and sequence data. Probably the most interesting is the vcfeval command which performs sophisticated comparison of VCF files.

Conventional tools attempt comparison by directly comparing variant positions, alleles, and genotypes, however they are inherently unable to deal with differences in representation that commonly arise, particularly when dealing with complex variants or when comparing variants produced by different callers. More details are in this presentation on slideshare and this manuscript on bioRxiv. Comparison approaches based on normalization or decomposition can alleviate these problem but often fail to deal with more complex situations.

RTG vcfeval performs variant comparison at the haplotype level, that is, it determines whether the genotypes asserted in the VCFs under comparison result in the same genomic sequence when applied to the reference genome. This in itself is a non-trivial problem and naive approaches face a combinatorial explosion to determine the most accurate analysis. To date, no other tool is capable of performing this analysis as accurately and as fast as RTG vcfeval. RTG developed vcfeval for in-house use in 2010, and through our collaborations we found this tool to be highly useful outside of RTG. RTG vcfeval outputs VCF files containing the results of comparison, summary metrics, and ROC curve data files.

In conjunction with vcfeval, the rocplot command provides an easy way to interactively examine the ROC curves from one or more vcfeval runs as an aid to selecting appropriate scoring attributes and filtering thresholds. A quick screen capture is shown below:


In order to encourage wider adoption of best-practise methods for variant comparison and benchmarking, Real Time Genomics made RTG Tools freely available, and now this includes the source code under an OSI approved open source licence. RTG Tools are mature, well tested, and under ongoing development.

RTG Tools is available pre-packaged directly from our website, or follow the instructions below to build from this repository.

If you have a need for variant calling or metagenomics analysis, please consider our full analysis suite RTG Core.


A user manual is included within the installation in both PDF and HTML versions. These may also be viewed online (HTML, PDF).

You can use the commands in RTG Tools to format your own reference datasets, or download common pre-formatted references from our website.

An rtg-users discussion group is now available for general questions, tips, and other discussions.

To be informed of new software releases, subscribe to the low-traffic rtg-announce group.

Prerequisites for building from source

  • Java 1.8 or later
  • apache ant 1.9 or later

Check out source code for RTG Tools

$ git clone
$ cd rtg-tools

Compile / run unit tests

$ ant runalltests

Build RTG Tools package

To build the RTG Tools package which can be locally installed and run:

$ ant zip-nojre

This will create an installation zip file under dist.


Uncompress the installation zip:

$ cd /my/install/dir/
$ unzip /path/to/rtg-tools/dist/

Follow the instructions contained in the README.txt. This build will use the system Java by default, so ensure that it is Java 1.8 or later.

For a quick demonstration of the features of RTG Tools for simulation and VCF processing on data generated from scratch, run the script contained in the scripts subdirectory of the installation directory:

$ cd /my/install/dir/rtg-tools-VERSION/
$ ./scripts/ $PWD/rtg