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Develop and Validate Geoduck VTG qPCR assay #491

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sr320 opened this Issue Nov 16, 2018 · 3 comments

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sr320 commented Nov 16, 2018

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sr320 commented Nov 20, 2018

I think step one might be identifying the canonical transcriptome and finding the vitellogenin gene(s)

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kubu4 commented Nov 21, 2018

I put together this transcriptome a while ago:

http://owl.fish.washington.edu/Athaliana/20180827_trinity_geoduck_RNAseq/Trinity.fasta

But, it never got put up on the Genomic Resources wiki as the canonical version. 😭

Notebook of assembly is here:

https://robertslab.github.io//sams-notebook/2018/09/04/transcriptome-assembly-geoduck-rnaseq-data.html

Regardless, I'll just use this to find some vitellogenin genes.

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kubu4 commented Nov 27, 2018

Update: Transdecoder finished late last week, so I'll be designing and ordering some VTG primers today.

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