dspFRET

Matlab code for analyzing TTTR data from PicoQuant. Typical application is analysis of single pair FRET measurements with pulsed interleaved excitation (PIE-FRET).

File Structure

• analyzePIE. Main analysis engine
• readHeader. Function that reads the header of a Symphotime TTTR binary file
• readCounts2. Speedup of over 100x from PicoQuant parser.
• calcMCS. calculates the MCS binning. Returns MCS data and the maps of photons into bins.

Output

• TCSPC histograms
• Photon counting histograms (PCH)
• MCS traces (also called burst traces)
• 1D histograms of TE, S, and tau
• 2D histograms of TE-S
• 2D histograms of TE-tau (new)