diff --git a/CHANGELOG.md b/CHANGELOG.md
index 95c2239935..b020b5d244 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -8,6 +8,7 @@
 - **bucket:** Make official buckets higher priority ([#5398](https://github.com/ScoopInstaller/Scoop/issues/5398))
 - **core:** Add `-Quiet` switch for `Invoke-ExternalCommand` ([#5346](https://github.com/ScoopInstaller/Scoop/issues/5346))
 - **core:** Allow global install of PowerShell modules ([#5611](https://github.com/ScoopInstaller/Scoop/issues/5611))
+- **path:** Isolate Scoop apps' PATH ([#5840](https://github.com/ScoopInstaller/Scoop/issues/5840))
 
 ### Bug Fixes
 
diff --git a/bin/uninstall.ps1 b/bin/uninstall.ps1
index 9e89481959..98b5c8d513 100644
--- a/bin/uninstall.ps1
+++ b/bin/uninstall.ps1
@@ -100,5 +100,8 @@ if ($purge) {
 }
 
 Remove-Path -Path (shimdir $global) -Global:$global
+if (get_config USE_ISOLATED_PATH) {
+    Remove-Path -Path ('%' + $scoopPathEnvVar + '%') -Global:$global
+}
 
 success 'Scoop has been uninstalled.'
diff --git a/lib/core.ps1 b/lib/core.ps1
index 37c96ebe43..cf60f27e4c 100644
--- a/lib/core.ps1
+++ b/lib/core.ps1
@@ -132,6 +132,9 @@ function set_config {
         $value = [System.Convert]::ToBoolean($value)
     }
 
+    # Initialize config's change
+    Complete-ConfigChange -Name $name -Value $value
+
     if ($null -eq $scoopConfig.$name) {
         $scoopConfig | Add-Member -MemberType NoteProperty -Name $name -Value $value
     } else {
@@ -147,6 +150,74 @@ function set_config {
     return $scoopConfig
 }
 
+function Complete-ConfigChange {
+    [CmdletBinding()]
+    param (
+        [Parameter(Mandatory, Position = 0)]
+        [string]
+        $Name,
+        [Parameter(Mandatory, Position = 1)]
+        [AllowEmptyString()]
+        [string]
+        $Value
+    )
+
+    if ($Name -eq 'use_isolated_path') {
+        $oldValue = get_config USE_ISOLATED_PATH
+        if ($Value -eq $oldValue) {
+            return
+        } else {
+            $currPathEnvVar = $scoopPathEnvVar
+        }
+        . "$PSScriptRoot\..\lib\system.ps1"
+
+        if ($Value -eq $false -or $Value -eq '') {
+            info 'Turn off Scoop isolated path... This may take a while, please wait.'
+            $movedPath = Get-EnvVar -Name $currPathEnvVar
+            if ($movedPath) {
+                Add-Path -Path $movedPath -Quiet
+                Remove-Path -Path ('%' + $currPathEnvVar + '%') -Quiet
+                Set-EnvVar -Name $currPathEnvVar -Quiet
+            }
+            if (is_admin) {
+                $movedPath = Get-EnvVar -Name $currPathEnvVar -Global
+                if ($movedPath) {
+                    Add-Path -Path $movedPath -Global -Quiet
+                    Remove-Path -Path ('%' + $currPathEnvVar + '%') -Global -Quiet
+                    Set-EnvVar -Name $currPathEnvVar -Global -Quiet
+                }
+            }
+        } else {
+            $newPathEnvVar = if ($Value -eq $true) {
+                'SCOOP_PATH'
+            } else {
+                $Value.ToUpperInvariant()
+            }
+            info "Turn on Scoop isolated path ('$newPathEnvVar')... This may take a while, please wait."
+            $movedPath = Remove-Path -Path "$scoopdir\apps\*" -TargetEnvVar $currPathEnvVar -Quiet -PassThru
+            if ($movedPath) {
+                Add-Path -Path $movedPath -TargetEnvVar $newPathEnvVar -Quiet
+                Add-Path -Path ('%' + $newPathEnvVar + '%') -Quiet
+                if ($currPathEnvVar -ne 'PATH') {
+                    Remove-Path -Path ('%' + $currPathEnvVar + '%') -Quiet
+                    Set-EnvVar -Name $currPathEnvVar -Quiet
+                }
+            }
+            if (is_admin) {
+                $movedPath = Remove-Path -Path "$globaldir\apps\*" -TargetEnvVar $currPathEnvVar -Global -Quiet -PassThru
+                if ($movedPath) {
+                    Add-Path -Path $movedPath -TargetEnvVar $newPathEnvVar -Global -Quiet
+                    Add-Path -Path ('%' + $newPathEnvVar + '%') -Global -Quiet
+                    if ($currPathEnvVar -ne 'PATH') {
+                        Remove-Path -Path ('%' + $currPathEnvVar + '%') -Global -Quiet
+                        Set-EnvVar -Name $currPathEnvVar -Global -Quiet
+                    }
+                }
+            }
+        }
+    }
+}
+
 function setup_proxy() {
     # note: '@' and ':' in password must be escaped, e.g. 'p@ssword' -> p\@ssword'
     $proxy = get_config PROXY
@@ -303,7 +374,7 @@ function filesize($length) {
     } else {
         if ($null -eq $length) {
             $length = 0
-       }
+        }
         "$($length) B"
     }
 }
@@ -1350,6 +1421,13 @@ $globaldir = $env:SCOOP_GLOBAL, (get_config GLOBAL_PATH), "$([System.Environment
 #       Use at your own risk.
 $cachedir = $env:SCOOP_CACHE, (get_config CACHE_PATH), "$scoopdir\cache" | Where-Object { $_ } | Select-Object -First 1 | Get-AbsolutePath
 
+# Scoop apps' PATH Environment Variable
+$scoopPathEnvVar = switch (get_config USE_ISOLATED_PATH) {
+    { $_ -is [string] } { $_.ToUpperInvariant() }
+    $true { 'SCOOP_PATH' }
+    default { 'PATH' }
+}
+
 # OS information
 $WindowsBuild = [System.Environment]::OSVersion.Version.Build
 
diff --git a/lib/install.ps1 b/lib/install.ps1
index 19f8d9b3f6..12d4220015 100644
--- a/lib/install.ps1
+++ b/lib/install.ps1
@@ -906,19 +906,11 @@ function env_add_path($manifest, $dir, $global, $arch) {
     $env_add_path = arch_specific 'env_add_path' $manifest $arch
     $dir = $dir.TrimEnd('\')
     if ($env_add_path) {
-        # GH-3785: Add path in ascending order.
-        [Array]::Reverse($env_add_path)
-        $env_add_path | Where-Object { $_ } | ForEach-Object {
-            if ($_ -eq '.') {
-                $path_dir = $dir
-            } else {
-                $path_dir = Join-Path $dir $_
-            }
-            if (!(is_in_dir $dir $path_dir)) {
-                abort "Error in manifest: env_add_path '$_' is outside the app directory."
-            }
-            Add-Path -Path $path_dir -Global:$global -Force
+        if (get_config USE_ISOLATED_PATH) {
+            Add-Path -Path ('%' + $scoopPathEnvVar + '%') -Global:$global
         }
+        $path = $env_add_path.Where({ $_ }).ForEach({ Join-Path $dir $_ | Get-AbsolutePath }).Where({ is_in_dir $dir $_ })
+        Add-Path -Path $path -TargetEnvVar $scoopPathEnvVar -Global:$global -Force
     }
 }
 
@@ -926,14 +918,9 @@ function env_rm_path($manifest, $dir, $global, $arch) {
     $env_add_path = arch_specific 'env_add_path' $manifest $arch
     $dir = $dir.TrimEnd('\')
     if ($env_add_path) {
-        $env_add_path | Where-Object { $_ } | ForEach-Object {
-            if ($_ -eq '.') {
-                $path_dir = $dir
-            } else {
-                $path_dir = Join-Path $dir $_
-            }
-            Remove-Path -Path $path_dir -Global:$global
-        }
+        $path = $env_add_path.Where({ $_ }).ForEach({ Join-Path $dir $_ | Get-AbsolutePath }).Where({ is_in_dir $dir $_ })
+        Remove-Path -Path $path -Global:$global # TODO: Remove after forced isolating Scoop path
+        Remove-Path -Path $path -TargetEnvVar $scoopPathEnvVar -Global:$global
     }
 }
 
diff --git a/lib/system.ps1 b/lib/system.ps1
index 2a572a9fde..affe2c5450 100644
--- a/lib/system.ps1
+++ b/lib/system.ps1
@@ -73,63 +73,79 @@ function Set-EnvVar {
     Publish-EnvVar
 }
 
-function Test-PathLikeEnvVar {
+function Split-PathLikeEnvVar {
     param(
-        [string]$Name,
+        [string[]]$Pattern,
         [string]$Path
     )
 
     if ($null -eq $Path -and $Path -eq '') {
-        return $false, $null
+        return $null, $null
     } else {
-        $strippedPath = $Path.Split(';', [System.StringSplitOptions]::RemoveEmptyEntries).Where({ $_ -ne $Name }) -join ';'
-        return ($strippedPath -ne $Path), $strippedPath
+        $splitPattern = $Pattern.Split(';', [System.StringSplitOptions]::RemoveEmptyEntries)
+        $splitPath = $Path.Split(';', [System.StringSplitOptions]::RemoveEmptyEntries)
+        $inPath = @()
+        foreach ($p in $splitPattern) {
+            $inPath += $splitPath.Where({ $_ -like $p })
+            $splitPath = $splitPath.Where({ $_ -notlike $p })
+        }
+        return ($inPath -join ';'), ($splitPath -join ';')
     }
 }
 
 function Add-Path {
     param(
-        [string]$Path,
+        [string[]]$Path,
+        [string]$TargetEnvVar = 'PATH',
         [switch]$Global,
-        [switch]$Force
+        [switch]$Force,
+        [switch]$Quiet
     )
 
-    if (!$Path.Contains('%')) {
-        $Path = Get-AbsolutePath $Path
-    }
     # future sessions
-    $inPath, $strippedPath = Test-PathLikeEnvVar $Path (Get-EnvVar -Name 'PATH' -Global:$Global)
+    $inPath, $strippedPath = Split-PathLikeEnvVar $Path (Get-EnvVar -Name $TargetEnvVar -Global:$Global)
     if (!$inPath -or $Force) {
-        Write-Output "Adding $(friendly_path $Path) to $(if ($Global) {'global'} else {'your'}) path."
-        Set-EnvVar -Name 'PATH' -Value (@($Path, $strippedPath) -join ';') -Global:$Global
+        if (!$Quiet) {
+            $Path | ForEach-Object {
+                Write-Host "Adding $(friendly_path $_) to $(if ($Global) {'global'} else {'your'}) path."
+            }
+        }
+        Set-EnvVar -Name $TargetEnvVar -Value ((@($Path) + $strippedPath) -join ';') -Global:$Global
     }
     # current session
-    $inPath, $strippedPath = Test-PathLikeEnvVar $Path $env:PATH
+    $inPath, $strippedPath = Split-PathLikeEnvVar $Path $env:PATH
     if (!$inPath -or $Force) {
-        $env:PATH = @($Path, $strippedPath) -join ';'
+        $env:PATH = (@($Path) + $strippedPath) -join ';'
     }
 }
 
 function Remove-Path {
     param(
-        [string]$Path,
-        [switch]$Global
+        [string[]]$Path,
+        [string]$TargetEnvVar = 'PATH',
+        [switch]$Global,
+        [switch]$Quiet,
+        [switch]$PassThru
     )
 
-    if (!$Path.Contains('%')) {
-        $Path = Get-AbsolutePath $Path
-    }
     # future sessions
-    $inPath, $strippedPath = Test-PathLikeEnvVar $Path (Get-EnvVar -Name 'PATH' -Global:$Global)
+    $inPath, $strippedPath = Split-PathLikeEnvVar $Path (Get-EnvVar -Name $TargetEnvVar -Global:$Global)
     if ($inPath) {
-        Write-Output "Removing $(friendly_path $Path) from $(if ($Global) {'global'} else {'your'}) path."
-        Set-EnvVar -Name 'PATH' -Value $strippedPath -Global:$Global
+        if (!$Quiet) {
+            $Path | ForEach-Object {
+                Write-Host "Removing $(friendly_path $_) from $(if ($Global) {'global'} else {'your'}) path."
+            }
+        }
+        Set-EnvVar -Name $TargetEnvVar -Value $strippedPath -Global:$Global
     }
     # current session
-    $inPath, $strippedPath = Test-PathLikeEnvVar $Path $env:PATH
-    if ($inPath) {
+    $inSessionPath, $strippedPath = Split-PathLikeEnvVar $Path $env:PATH
+    if ($inSessionPath) {
         $env:PATH = $strippedPath
     }
+    if ($PassThru) {
+        return $inPath
+    }
 }
 
 ## Deprecated functions
@@ -145,8 +161,8 @@ function env($name, $global, $val) {
 }
 
 function strip_path($orig_path, $dir) {
-    Show-DeprecatedWarning $MyInvocation 'Test-PathLikeEnvVar'
-    Test-PathLikeEnvVar -Name $dir -Path $orig_path
+    Show-DeprecatedWarning $MyInvocation 'Split-PathLikeEnvVar'
+    Split-PathLikeEnvVar -Name $dir -Path $orig_path
 }
 
 function add_first_in_path($dir, $global) {
diff --git a/libexec/scoop-config.ps1 b/libexec/scoop-config.ps1
index dbc1925305..ff0bff3eee 100644
--- a/libexec/scoop-config.ps1
+++ b/libexec/scoop-config.ps1
@@ -115,6 +115,11 @@
 #       Nightly version is formatted as 'nightly-yyyyMMdd' and will be updated after one day if this is set to $true.
 #       Otherwise, nightly version will not be updated unless `--force` is used.
 #
+# use_isolated_path: $true|$false|[string]
+#       When set to $true, Scoop will use `SCOOP_PATH` environment variable to store apps' `PATH`s.
+#       When set to arbitrary non-empty string, Scoop will use that string as the environment variable name instead.
+#       This is useful when you want to isolate Scoop from the system `PATH`.
+#
 # ARIA2 configuration
 # -------------------
 #
diff --git a/libexec/scoop-reset.ps1 b/libexec/scoop-reset.ps1
index e073cd4dfb..aeef05cc83 100644
--- a/libexec/scoop-reset.ps1
+++ b/libexec/scoop-reset.ps1
@@ -80,6 +80,9 @@ $apps | ForEach-Object {
     $dir = link_current $dir
     create_shims $manifest $dir $global $architecture
     create_startmenu_shortcuts $manifest $dir $global $architecture
+    # unset all potential old env before re-adding
+    env_rm_path $manifest $dir $global $architecture
+    env_rm $manifest $global $architecture
     env_add_path $manifest $dir $global $architecture
     env_set $manifest $dir $global $architecture
     # unlink all potential old link before re-persisting
diff --git a/test/Scoop-Install.Tests.ps1 b/test/Scoop-Install.Tests.ps1
index 130d6a7517..966c2c0c50 100644
--- a/test/Scoop-Install.Tests.ps1
+++ b/test/Scoop-Install.Tests.ps1
@@ -46,13 +46,10 @@ Describe 'env add and remove path' -Tag 'Scoop', 'Windows' {
     BeforeAll {
         # test data
         $manifest = @{
-            'env_add_path' = @('foo', 'bar')
+            'env_add_path' = @('foo', 'bar', '.', '..')
         }
         $testdir = Join-Path $PSScriptRoot 'path-test-directory'
         $global = $false
-
-        # store the original path to prevent leakage of tests
-        $origPath = $env:PATH
     }
 
     It 'should concat the correct path' {
@@ -61,12 +58,16 @@ Describe 'env add and remove path' -Tag 'Scoop', 'Windows' {
 
         # adding
         env_add_path $manifest $testdir $global
-        Assert-MockCalled Add-Path -Times 1 -ParameterFilter { $Path -like "$testdir\foo" }
-        Assert-MockCalled Add-Path -Times 1 -ParameterFilter { $Path -like "$testdir\bar" }
+        Should -Invoke -CommandName Add-Path -Times 1 -ParameterFilter { $Path -like "$testdir\foo" }
+        Should -Invoke -CommandName Add-Path -Times 1 -ParameterFilter { $Path -like "$testdir\bar" }
+        Should -Invoke -CommandName Add-Path -Times 1 -ParameterFilter { $Path -like $testdir }
+        Should -Invoke -CommandName Add-Path -Times 0 -ParameterFilter { $Path -like $PSScriptRoot }
 
         env_rm_path $manifest $testdir $global
-        Assert-MockCalled Remove-Path -Times 1 -ParameterFilter { $Path -like "$testdir\foo" }
-        Assert-MockCalled Remove-Path -Times 1 -ParameterFilter { $Path -like "$testdir\bar" }
+        Should -Invoke -CommandName Remove-Path -Times 1 -ParameterFilter { $Path -like "$testdir\foo" }
+        Should -Invoke -CommandName Remove-Path -Times 1 -ParameterFilter { $Path -like "$testdir\bar" }
+        Should -Invoke -CommandName Remove-Path -Times 1 -ParameterFilter { $Path -like $testdir }
+        Should -Invoke -CommandName Remove-Path -Times 0 -ParameterFilter { $Path -like $PSScriptRoot }
     }
 }