diff --git a/.changeset/loud-cycles-check.md b/.changeset/loud-cycles-check.md
new file mode 100644
index 00000000000..dedbf545592
--- /dev/null
+++ b/.changeset/loud-cycles-check.md
@@ -0,0 +1,5 @@
+---
+'@shopify/polaris': patch
+---
+
+Fixed Navigation item secondaryActions alignment in mobile when floating actions are enabled
diff --git a/polaris-react/src/components/Navigation/Navigation.scss b/polaris-react/src/components/Navigation/Navigation.scss
index ba511e360e4..6f20a15d932 100644
--- a/polaris-react/src/components/Navigation/Navigation.scss
+++ b/polaris-react/src/components/Navigation/Navigation.scss
@@ -298,6 +298,9 @@ $disabled-fade: 0.6;
 .ItemWithFloatingActions {
   // stylelint-disable-next-line -- required for absolute positioning of actions
   position: relative;
+  // stylelint-disable-next-line -- position actions to to the right of label
+  display: flex;
+  flex-wrap: nowrap;
 
   .SecondaryActions {
     margin-right: 0;