diff --git a/src/navigate/controller/controller.py b/src/navigate/controller/controller.py
index 014387534..7a3de93e1 100644
--- a/src/navigate/controller/controller.py
+++ b/src/navigate/controller/controller.py
@@ -440,6 +440,17 @@ def update_experiment_setting(self):
 
         """
         self.camera_setting_controller.update_experiment_values()
+
+        # set waveform template
+        if self.acquire_bar_controller.mode in ["live", "single", "z-stack"]:
+            camera_setting = self.configuration["experiment"]["CameraParameters"]
+            if camera_setting["sensor_mode"] == "Light-Sheet" and camera_setting[
+                "readout_direction"
+            ] in ["Bidirectional", "Rev. Bidirectional"]:
+                self.waveform_tab_controller.set_waveform_template("Bidirectional")
+            else:
+                self.waveform_tab_controller.set_waveform_template("Default")
+
         # update multi-positions
         positions = self.multiposition_tab_controller.get_positions()
         update_config_dict(
@@ -752,9 +763,11 @@ def execute(self, command, *args):
             )
 
             # Save the waveform_constants.yaml file.
-            save_yaml_file(file_directory=file_directory,
-                           content_dict=self.configuration['waveform_constants'],
-                           filename="waveform_constants.yml")
+            save_yaml_file(
+                file_directory=file_directory,
+                content_dict=self.configuration["waveform_constants"],
+                filename="waveform_constants.yml",
+            )
 
             self.camera_setting_controller.solvent = self.configuration["experiment"][
                 "Saving"
@@ -985,7 +998,6 @@ def capture_image(self, command, mode, *args):
                 )
                 self.execute("stop_acquire")
 
-
             # Display the Image in the View
             self.camera_view_controller.try_to_display_image(image_id=image_id)
             images_received += 1
diff --git a/src/navigate/controller/sub_controllers/waveform_tab_controller.py b/src/navigate/controller/sub_controllers/waveform_tab_controller.py
index b8ec197b7..0b9a07fbf 100644
--- a/src/navigate/controller/sub_controllers/waveform_tab_controller.py
+++ b/src/navigate/controller/sub_controllers/waveform_tab_controller.py
@@ -328,3 +328,20 @@ def set_mode(self, mode):
         """
         state = "normal" if mode == "stop" else "disabled"
         self.view.waveform_settings.inputs["waveform_template"].widget["state"] = state
+
+    def set_waveform_template(self, template_name):
+        """Set the waveform template name
+
+        Parameters
+        ----------
+        template_name : str
+            Set the waveform template name
+
+        Examples
+        --------
+        >>> self.set_waveform_template(template_name)
+        """
+        self.view.waveform_settings.inputs["waveform_template"].set(template_name)
+        self.parent_controller.configuration["experiment"]["MicroscopeState"][
+            "waveform_template"
+        ] = template_name
diff --git a/test/controller/test_controller.py b/test/controller/test_controller.py
index 83166531f..193624c3d 100644
--- a/test/controller/test_controller.py
+++ b/test/controller/test_controller.py
@@ -1,5 +1,6 @@
 from pathlib import Path
 from types import SimpleNamespace
+from unittest.mock import MagicMock
 
 import pytest
 
@@ -48,6 +49,11 @@ def controller(tk_root):
         waveform_templates_path,
         args,
     )
+    # To make sure the testcases won't hang on because of the model.event_queue
+    # The changes here won't affect other testcases,
+    # because the testcases from other files use DummyController and DummyModel instead of this controller fixture
+    controller.model = MagicMock()
+    controller.model.get_offset_variance_maps.return_value = (None, None)
 
     yield controller
 
@@ -89,3 +95,43 @@ def test_prepare_acquire_data(controller):
 def test_execute(controller):
     controller.execute("acquire", "single")
     assert True
+
+
+@pytest.mark.parametrize(
+    "acquisition_mode, sensor_mode, readout_direction, template_name, expected_template_name",
+    [
+        ("live", "Normal", "", "Bidirectional", "Default"),
+        ("z-stack", "Normal", "", "Bidirectional", "Default"),
+        ("customized", "Normal", "", "Bidirectional", "Bidirectional"),
+        ("live", "Light-Sheet", "Top-To-Bottom", "Bidirectional", "Default"),
+        ("live", "Light-Sheet", "Bidirectional", "Bidirectional", "Bidirectional"),
+        ("customized", "Light-Sheet", "Bidirectional", "Bidirectional", "Bidirectional",),
+        ("z-stack", "Light-Sheet", "Bidirectional", "Default", "Bidirectional"),
+        ("z-stack", "Light-Sheet", "Top-To-Bottom", "Default", "Default"),
+    ],
+)
+def test_waveform_template(
+    controller,
+    acquisition_mode,
+    sensor_mode,
+    readout_direction,
+    template_name,
+    expected_template_name,
+):
+    controller.configuration["experiment"]["MicroscopeState"][
+        "waveform_template"
+    ] = template_name
+    controller.configuration["experiment"]["MicroscopeState"][
+        "image_mode"
+    ] = acquisition_mode
+    controller.configuration["experiment"]["CameraParameters"]["number_of_pixels"] = 10
+    controller.populate_experiment_setting(in_initialize=True)
+
+    controller.camera_setting_controller.mode_widgets["Readout"].set(readout_direction)
+    controller.camera_setting_controller.mode_widgets["Sensor"].set(sensor_mode)
+    controller.update_experiment_setting()
+
+    assert (
+        controller.configuration["experiment"]["MicroscopeState"]["waveform_template"]
+        == expected_template_name
+    )