diff --git a/packages/vite/src/node/optimizer/esbuildDepPlugin.ts b/packages/vite/src/node/optimizer/esbuildDepPlugin.ts
index 71d8ed81c70c61..f53a905d16a5e3 100644
--- a/packages/vite/src/node/optimizer/esbuildDepPlugin.ts
+++ b/packages/vite/src/node/optimizer/esbuildDepPlugin.ts
@@ -23,6 +23,7 @@ const externalTypes = [
   // known SFC types
   'vue',
   'svelte',
+  'marko',
   // JSX/TSX may be configured to be compiled differently from how esbuild
   // handles it by default, so exclude them as well
   'jsx',
diff --git a/packages/vite/src/node/utils.ts b/packages/vite/src/node/utils.ts
index f259fb61ebe667..efa08c8c7b4474 100644
--- a/packages/vite/src/node/utils.ts
+++ b/packages/vite/src/node/utils.ts
@@ -106,7 +106,7 @@ export const isExternalUrl = (url: string): boolean => externalRE.test(url)
 export const dataUrlRE = /^\s*data:/i
 export const isDataUrl = (url: string): boolean => dataUrlRE.test(url)
 
-const knownJsSrcRE = /\.((j|t)sx?|mjs|vue)($|\?)/
+const knownJsSrcRE = /\.((j|t)sx?|mjs|vue|marko)($|\?)/
 export const isJSRequest = (url: string): boolean => {
   if (knownJsSrcRE.test(url)) {
     return true