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Drosophila Adult Brain Domains
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ItoJFRC2_w800
combinedIndexFiles
individualDomainFiles
maxProjectionViews
refData
template
LICENSE
README.md

README.md

Drosophila Adult Brain Template and Domains

This is the location of the main brain template created from JFRCtemplate2010 with labeled domains from the mask dated 19/08/13.

Folder Contence Description
templates Background neuropil template
refData index domain numbers to anatomy labels / IDs
combinedIndexFiles indexed domain label files
individualDomainFiles index domain labels split out as individual files.
maxProjectionViews z projection maximum projection views and thumbnails for each index.

Recommended files

Kind File voxel size dims
nc82 template JFRCtemplate2010.nrrd 0.622088 µm isotropic 1024 x 512 x 218
Label Field JFRCtempate2010.mask130819_Original.nrrd 0.622088 µm isotropic 1024 x 512 x 218

nrrd format images can be opened in Fiji/ImageJ, Vaa3d > 3.024, Amira (with plugin) and many medical imaging software packages.

Source Data

The source images for the template and mask files were:

file md5 voxel size dims
JFRCtemplate.tif 6e7f4e54cd630d389784132c7108a284 1 x 1 x 1 3 x 1024 x 512 x 218
JFRCtempate2010.mask130819.am d0a40b38d1a0045a423d947ebf1778d2 1 x 1 x 1.47 1024 x 512 x 218

Template data

JFRCtemplate.tif was generated by Arnim Jenett and colleagues at Janelia Farm as part of the FlyLight project reported in Jenett et al 2012. It contained 3 identical copies of the same image channel, an nc82 staining of a single female brain. This was 8 bit uncalibrated data. The original image was acquired with a 20x dry objective on a Zeiss confocal. The lateral resolution was 0.622088 and the slice spacing was 1 micron (uncorrected for air/mounting medium mismatch). This data was later upsampled to 0.622088 isotropic, with 218 slices in Z.

JFRC2

We have resaved this original data using the 0.622088 isotropic calibration and commonly refer to this as the JFRC2 template. It is available as JFRCtemplate2010.nrrd in nrrd format, which consists of a plain text header concatenated with a gzipped data block.

JFRCtemplate2010.nrrd header:

NRRD0004
# This NRRD file was generated by pynrrd
# on 2014-05-07 13:49:45(GMT).
# Complete NRRD file format specification at:
# http://teem.sourceforge.net/nrrd/format.html
type: uint8
dimension: 3
space dimension: 3
sizes: 1024 512 218
space directions: (0.622088,0.0,0.0) (0.0,0.622088,0.0) (0.0,0.0,0.622088)
endian: big
encoding: gzip
space units: "?m" "?m" "?m"

(nb this output was produced by unu head. Nrrd assumes that the header is ASCII encoded details here. However this header is implictly encoded in something like ISO-8859-1 for the µ (micro) symbol to work!

Label field data

The label field segmentation describing standardised neuropil domains was generated by Arnim Jenett (Janelia Farm Research Campus), Kazunori Shinomiya and Kei Ito (University of Tokyo) based on the template data described above.There have been at least two iterations of the label field data, the most recent dated 130819, JFRCtempate2010.mask130819.am.

The label field in Amira format was resaved as an 8 bit nrrd file with integer levels between 0 and 85, JFRCtempate2010.mask130819_Original.nrrd. The levels are defined in Original_Index.tsv.

JFRCtempate2010.mask130819_Original.nrrd header:

NRRD0004
# This NRRD file was generated by pynrrd
# on 2014-06-19 11:17:24(GMT).
# Complete NRRD file format specification at:
# http://teem.sourceforge.net/nrrd/format.html
type: uint8
dimension: 3
space dimension: 3
sizes: 1024 512 218
space directions: (0.622088,0.0,0.0) (0.0,0.622088,0.0) (0.0,0.0,0.622088)
endian: big
encoding: gzip
space units: "?m" "?m" "?m"
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