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Motif marker

This script was created in order to provide a tool that would enable the visualization of motifs within gene sequences. Given an input fasta file, and a reference file containing motifs to search for, the script will produce a scaled image containing the locations of the motifs provided.

Usage

To use this script, execute:

motif-mark-oop.py

with the following arguments:

-f --fasta file: input fasta file containing either RNA and/or DNA sequence reads. The script identifies exons based on character captilization.

-m --motifs file: file containing motifs to search for within each gene. Motif file should contain 1 motif per line and can handle any combination of pyrimadines and/or purines (i.e 'r' and 'y' are acceptable characters for purine and pyrimidine searching, respectively).

See example input motif file labled Fig_1_motifs.txt and fasta file labeled Figure_1.fasta for reference.

See Figure_1.png below for an example of the output of the script.

image