plantCmeMethods

Code for the quantitative analysis of plant clathrin-mediated endocytosis (CME).

From the paper;

"Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis"

Alexander Johnson, Nataliia Gnyliukh, Walter A. Kaufmann, Madhumitha Narasimhan, Grégory Vert, Sebastian Y. Bednarek, Jiří Friml Journal of Cell Science 2020 : jcs.248062 doi: 10.1242/jcs.248062 Published 2 July 2020

https://doi.org/10.1242/jcs.248062

Includes the analysis for – cell surface analysis of the dynamics of endocytosis proteins (single and dual channel data), inhibition of cell surface dynamics of single channel cell surface imaging and global uptake.

For further details on how to use please see the detailed PDF manuals.

Example data can be found at https://doi.org/10.5281/zenodo.3888530