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Add script to perform normalization and dimension reduction #203

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jashapiro opened this issue Aug 1, 2022 · 1 comment
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Add script to perform normalization and dimension reduction #203

jashapiro opened this issue Aug 1, 2022 · 1 comment
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@jashapiro
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We should add a new step to the workflow that takes the filtered SCE object and applies normalization and dimension reduction (UMAP), storing those results in the SCE that is finally published.

This step should immediately precede the QC report.

We should follow the same code steps that we use in https://github.com/AlexsLemonade/scpca-downstream-analyses, but I expect we will want to combine any additional filtering, normalization, and the UMAP calcualtion into a single script so it can run all together.

One question is whether we want to include filtering as specified by miQC, and any additional criteria. I had the thought that we could do this in a "soft" filter. In other words, we would do so before calculating UMAP, and then merge the calculated UMAP matrix in with the full data set, setting values to NA for cells that were filtered out. This allows us to keep the QC template as is, but still include UMAP plots for only those cells that pass additional filtering (avoiding a cluster of high-mito cells, for example)

@allyhawkins allyhawkins changed the title Add normalization and dimension reduction to workflow Add script to perform normalization and dimension reduction Aug 29, 2022
@allyhawkins allyhawkins self-assigned this Aug 29, 2022
@jashapiro
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closed by #207

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