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MuPeXI: Mutant Peptide eXtractor and Informer

Given a list of somatic mutations (VCF file) as input, MuPeXI returns a table containing all mutated peptides (neo-peptides) of user-defined lengths, along with several pieces of information relevant for identifying which of these neo-peptides are likely to serve as neo-epitopes.

NEW: MuPeXI is now tested and compatible for suquencing data of murine origin.


Anne-Mette Bjerregaard and Aron C. Eklund


MuPeXI is freely available for academic users (see License) , other users are requested to contact CBS Software Package Manager at


Original paper

Bjerregaard AM, Nielsen M, Hadrup SR, Szallasi Z, Eklund AC.
MuPeXI: Prediction of neo-epitopes from tumor sequencing data.
Cancer Immunol Immunother. 2017 Apr 20. doi: 10.1007/s00262-017-2001-3.
PubMed ID: 28429069
You can read the paper here:

Murine compatible version

For use of the murine species option additonal citation of the following would be highly appreciated. Bjerregaard AM, Pedersen TK, Marquard AM, Hadrup SR. Prediction of neoepitopes from murine sequencing data. Cancer Immunol Immunother. 2018 Oct 05. doi: 10.1007/s00262-018-2254-5. PubMed ID: 30291365 You can read the paper here:

Web servers:

For limited data, MuPeXI can be run on our human specific web server and mouse specific web server



MuPeXI currently runs only on x86_64 machines running Linux or Darwin.

Required software:

Required Python modules:

Note: These modules are already included if using Python through Anaconda.

Optional software, required only for liftover from HG19


  1. Install all software listed above.

  2. Download or clone the MuPeXI repository to your local system

     git clone
  3. Obtain the reference files from GRCh38. These include cDNA, peptide and COSMIC files; see the References section in the user manual for a detailed description.

  4. Fill in the config.ini file

    • Provide the full path to NetMHCpan and VEP.
    • Provide the full path to the reference files:
      • cDNA
      • peptide
      • COSMIC

    Additional peptide references and liftover paths can be provided in the config.ini file; see the user manual for detailed information. Instructions on how to fill in the config.ini file are found within the file. config.ini is automatically found if it is in the same directory as script, but it can also be placed elsewhere and specified by the -c option.


Here is a simple example in which somatic mutation calls and gene expression data are provided, and MHC binding is predicted for HLA types HLA-A01:01 and HLA-B08:01.

path/to/ -v mutations.vcf -a HLA-A01:01,HLA-B08:01 -e expression.tsv

MuPeXI can be used for both peptide extraction, giving immunogenicity information for peptide selection (the default), and for generation of a FASTA-formatted mutant-peptide file suitable for input to mass spectrometry peptide search software (with the -f option).

All options can be displayed using the usage information with the -h option:

path/to/ -h

User Manual

For detailed information about usage, input and output files, test examples and data preparation read the MuPeXI User Manual


  • We pronounce it moo-PECKS-ee


Anne-Mette Bjerregaard


Aron Charles Eklund

Department of Bio and Health Informatics
Technical University of Denmark

Algorithmic Flow Chart


MuPeXI: the mutant peptide extractor and informer, a tool for predicting neo-epitopes from tumor sequencing data.







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Contributors 4