diff --git a/packages/plugin-segment/src/plugin.ts b/packages/plugin-segment/src/plugin.ts
index ebc99d9e..4a5060d5 100644
--- a/packages/plugin-segment/src/plugin.ts
+++ b/packages/plugin-segment/src/plugin.ts
@@ -15,7 +15,7 @@ export const segmentIntegrationPlugin: SegmentIntegrationPlugin = (
     return options.instance || snippetInstance(options.instanceKey);
   };
   getInstance();
-  let initialized = false;
+  let ready = false;
   const plugin: IntegrationPlugin = {
     name: '@amplitude/experiment-plugin-segment',
     type: 'integration',
@@ -23,7 +23,7 @@ export const segmentIntegrationPlugin: SegmentIntegrationPlugin = (
       const instance = getInstance();
       return new Promise<void>((resolve) => {
         instance.ready(() => {
-          initialized = true;
+          ready = true;
           resolve();
         });
         // If the segment SDK is installed via the @segment/analytics-next npm
@@ -31,8 +31,8 @@ export const segmentIntegrationPlugin: SegmentIntegrationPlugin = (
         if (!options.instance) {
           const interval = safeGlobal.setInterval(() => {
             const instance = getInstance();
-            if (instance.initialized || instance.instance?.initialized) {
-              initialized = true;
+            if (instance.initialized) {
+              ready = true;
               safeGlobal.clearInterval(interval);
               resolve();
             }
@@ -42,7 +42,7 @@ export const segmentIntegrationPlugin: SegmentIntegrationPlugin = (
     },
     getUser(): ExperimentUser {
       const instance = getInstance();
-      if (initialized) {
+      if (ready) {
         return {
           user_id: instance.user().id(),
           device_id: instance.user().anonymousId(),
@@ -60,7 +60,7 @@ export const segmentIntegrationPlugin: SegmentIntegrationPlugin = (
     },
     track(event: ExperimentEvent): boolean {
       const instance = getInstance();
-      if (!initialized) return false;
+      if (!ready) return false;
       instance.track(event.eventType, event.eventProperties);
       return true;
     },
diff --git a/packages/plugin-segment/src/snippet.ts b/packages/plugin-segment/src/snippet.ts
index f5859449..c4e3b97f 100644
--- a/packages/plugin-segment/src/snippet.ts
+++ b/packages/plugin-segment/src/snippet.ts
@@ -17,6 +17,10 @@ export const snippetInstance = (
   // Create a queue, but don't obliterate an existing one!
   const analytics = (safeGlobal[key] = safeGlobal[key] || []);
 
+  // Return the actual instance if the global analytics is nested in an instance.
+  if (analytics.instance && analytics.instance.initialize) {
+    return analytics.instance;
+  }
   // If the real analytics.js is already on the page return.
   if (analytics.initialize) {
     return analytics;