diff --git a/packages/angular/build/src/builders/unit-test/runners/vitest/plugins.ts b/packages/angular/build/src/builders/unit-test/runners/vitest/plugins.ts
index c375d182750d..04554955cd18 100644
--- a/packages/angular/build/src/builders/unit-test/runners/vitest/plugins.ts
+++ b/packages/angular/build/src/builders/unit-test/runners/vitest/plugins.ts
@@ -119,6 +119,7 @@ export async function createVitestConfigPlugin(
           globals: true,
           // Default to `false` to align with the Karma/Jasmine experience.
           isolate: false,
+          sequence: { setupFiles: 'list' },
         },
         optimizeDeps: {
           noDiscovery: true,
diff --git a/tests/legacy-cli/e2e/tests/vitest/browser-playwright.ts b/tests/legacy-cli/e2e/tests/vitest/browser-playwright.ts
new file mode 100644
index 000000000000..9b22b81736e4
--- /dev/null
+++ b/tests/legacy-cli/e2e/tests/vitest/browser-playwright.ts
@@ -0,0 +1,34 @@
+import assert from 'node:assert/strict';
+import { applyVitestBuilder } from '../../utils/vitest';
+import { exec, ng } from '../../utils/process';
+import { installPackage } from '../../utils/packages';
+import { writeFile } from '../../utils/fs';
+
+export default async function (): Promise<void> {
+  await applyVitestBuilder();
+  await installPackage('playwright@1');
+  await installPackage('@vitest/browser-playwright@4');
+  await exec('npx', 'playwright', 'install', 'chromium', '--only-shell');
+
+  await ng('generate', 'component', 'my-comp');
+
+  await writeFile(
+    'src/setup1.ts',
+    `
+      import { getTestBed } from '@angular/core/testing';
+
+      getTestBed().configureTestingModule({});
+    `,
+  );
+
+  const { stdout } = await ng(
+    'test',
+    '--no-watch',
+    '--browsers',
+    'chromiumHeadless',
+    '--setup-files',
+    'src/setup1.ts',
+  );
+
+  assert.match(stdout, /2 passed/, 'Expected 2 tests to pass.');
+}
diff --git a/tests/legacy-cli/e2e/tests/vitest/browser-webdriverio.ts b/tests/legacy-cli/e2e/tests/vitest/browser-webdriverio.ts
new file mode 100644
index 000000000000..4ea1b913c3b0
--- /dev/null
+++ b/tests/legacy-cli/e2e/tests/vitest/browser-webdriverio.ts
@@ -0,0 +1,33 @@
+import assert from 'node:assert/strict';
+import { applyVitestBuilder } from '../../utils/vitest';
+import { ng } from '../../utils/process';
+import { installPackage } from '../../utils/packages';
+import { writeFile } from '../../utils/fs';
+
+export default async function (): Promise<void> {
+  await applyVitestBuilder();
+  await installPackage('webdriverio@9');
+  await installPackage('@vitest/browser-webdriverio@4');
+
+  await ng('generate', 'component', 'my-comp');
+
+  await writeFile(
+    'src/setup1.ts',
+    `
+    import { getTestBed } from '@angular/core/testing';
+
+    getTestBed().configureTestingModule({});
+  `,
+  );
+
+  const { stdout } = await ng(
+    'test',
+    '--no-watch',
+    '--browsers',
+    'chromeHeadless',
+    '--setup-files',
+    'src/setup1.ts',
+  );
+
+  assert.match(stdout, /2 passed/, 'Expected 2 tests to pass.');
+}