diff --git a/airflow/providers/google/cloud/operators/dataflow.py b/airflow/providers/google/cloud/operators/dataflow.py
index d650775b9967e..eeec715edfc35 100644
--- a/airflow/providers/google/cloud/operators/dataflow.py
+++ b/airflow/providers/google/cloud/operators/dataflow.py
@@ -600,7 +600,7 @@ def __init__(
         options: dict[str, Any] | None = None,
         dataflow_default_options: dict[str, Any] | None = None,
         parameters: dict[str, str] | None = None,
-        location: str = DEFAULT_DATAFLOW_LOCATION,
+        location: str | None = None,
         gcp_conn_id: str = "google_cloud_default",
         poll_sleep: int = 10,
         impersonation_chain: str | Sequence[str] | None = None,
@@ -690,7 +690,7 @@ def set_current_job(current_job):
             trigger=TemplateJobStartTrigger(
                 project_id=self.project_id,
                 job_id=job_id,
-                location=self.location,
+                location=self.location if self.location else DEFAULT_DATAFLOW_LOCATION,
                 gcp_conn_id=self.gcp_conn_id,
                 poll_sleep=self.poll_sleep,
                 impersonation_chain=self.impersonation_chain,
diff --git a/tests/providers/google/cloud/hooks/test_dataflow.py b/tests/providers/google/cloud/hooks/test_dataflow.py
index f514b2705b0b4..c17358ba78cea 100644
--- a/tests/providers/google/cloud/hooks/test_dataflow.py
+++ b/tests/providers/google/cloud/hooks/test_dataflow.py
@@ -330,6 +330,50 @@ def test_start_python_dataflow_with_custom_region_as_parameter(
             job_id=mock.ANY, job_name=job_name, location=TEST_LOCATION
         )
 
+    @mock.patch(DATAFLOW_STRING.format("uuid.uuid4"))
+    @mock.patch(DATAFLOW_STRING.format("DataflowHook.wait_for_done"))
+    @mock.patch(DATAFLOW_STRING.format("process_line_and_extract_dataflow_job_id_callback"))
+    def test_start_python_dataflow_with_no_custom_region_or_region(
+        self, mock_callback_on_job_id, mock_dataflow_wait_for_done, mock_uuid
+    ):
+        mock_beam_start_python_pipeline = self.dataflow_hook.beam_hook.start_python_pipeline
+        mock_uuid.return_value = MOCK_UUID
+        on_new_job_id_callback = MagicMock()
+        py_requirements = ["pandas", "numpy"]
+        job_name = f"{JOB_NAME}-{MOCK_UUID_PREFIX}"
+
+        passed_variables = copy.deepcopy(DATAFLOW_VARIABLES_PY)
+
+        with pytest.warns(AirflowProviderDeprecationWarning, match="This method is deprecated"):
+            self.dataflow_hook.start_python_dataflow(
+                job_name=JOB_NAME,
+                variables=passed_variables,
+                dataflow=PY_FILE,
+                py_options=PY_OPTIONS,
+                py_interpreter=DEFAULT_PY_INTERPRETER,
+                py_requirements=py_requirements,
+                on_new_job_id_callback=on_new_job_id_callback,
+            )
+
+        expected_variables = copy.deepcopy(DATAFLOW_VARIABLES_PY)
+        expected_variables["job_name"] = job_name
+        expected_variables["region"] = DEFAULT_DATAFLOW_LOCATION
+
+        mock_callback_on_job_id.assert_called_once_with(on_new_job_id_callback)
+        mock_beam_start_python_pipeline.assert_called_once_with(
+            variables=expected_variables,
+            py_file=PY_FILE,
+            py_interpreter=DEFAULT_PY_INTERPRETER,
+            py_options=PY_OPTIONS,
+            py_requirements=py_requirements,
+            py_system_site_packages=False,
+            process_line_callback=mock_callback_on_job_id.return_value,
+        )
+
+        mock_dataflow_wait_for_done.assert_called_once_with(
+            job_id=mock.ANY, job_name=job_name, location=DEFAULT_DATAFLOW_LOCATION
+        )
+
     @mock.patch(DATAFLOW_STRING.format("uuid.uuid4"))
     @mock.patch(DATAFLOW_STRING.format("DataflowHook.wait_for_done"))
     @mock.patch(DATAFLOW_STRING.format("process_line_and_extract_dataflow_job_id_callback"))
diff --git a/tests/providers/google/cloud/operators/test_dataflow.py b/tests/providers/google/cloud/operators/test_dataflow.py
index c68938042ffb2..72db54c109f11 100644
--- a/tests/providers/google/cloud/operators/test_dataflow.py
+++ b/tests/providers/google/cloud/operators/test_dataflow.py
@@ -76,6 +76,7 @@
     },
 }
 TEST_LOCATION = "custom-location"
+TEST_REGION = "custom-region"
 TEST_PROJECT = "test-project"
 TEST_SQL_JOB_NAME = "test-sql-job-name"
 TEST_DATASET = "test-dataset"
@@ -534,6 +535,42 @@ def test_validation_deferrable_params_raises_error(self):
         with pytest.raises(ValueError):
             DataflowTemplatedJobStartOperator(**init_kwargs)
 
+    @mock.patch("airflow.providers.google.cloud.operators.dataflow.DataflowHook.start_template_dataflow")
+    def test_start_with_custom_region(self, dataflow_mock):
+        init_kwargs = {
+            "task_id": TASK_ID,
+            "template": TEMPLATE,
+            "dataflow_default_options": {
+                "region": TEST_REGION,
+            },
+            "poll_sleep": POLL_SLEEP,
+            "wait_until_finished": True,
+            "cancel_timeout": CANCEL_TIMEOUT,
+        }
+        operator = DataflowTemplatedJobStartOperator(**init_kwargs)
+        operator.execute(None)
+        assert dataflow_mock.called
+        _, kwargs = dataflow_mock.call_args_list[0]
+        assert kwargs["variables"]["region"] == TEST_REGION
+        assert kwargs["location"] is None
+
+    @mock.patch("airflow.providers.google.cloud.operators.dataflow.DataflowHook.start_template_dataflow")
+    def test_start_with_location(self, dataflow_mock):
+        init_kwargs = {
+            "task_id": TASK_ID,
+            "template": TEMPLATE,
+            "location": TEST_LOCATION,
+            "poll_sleep": POLL_SLEEP,
+            "wait_until_finished": True,
+            "cancel_timeout": CANCEL_TIMEOUT,
+        }
+        operator = DataflowTemplatedJobStartOperator(**init_kwargs)
+        operator.execute(None)
+        assert dataflow_mock.called
+        _, kwargs = dataflow_mock.call_args_list[0]
+        assert not kwargs["variables"]
+        assert kwargs["location"] == TEST_LOCATION
+
 
 class TestDataflowStartFlexTemplateOperator:
     @pytest.fixture