diff --git a/packages/javascript/.changesets/fix-matching-on-backtrace-paths-containing-spaces.md b/packages/javascript/.changesets/fix-matching-on-backtrace-paths-containing-spaces.md
new file mode 100644
index 00000000..4de2d2d3
--- /dev/null
+++ b/packages/javascript/.changesets/fix-matching-on-backtrace-paths-containing-spaces.md
@@ -0,0 +1,8 @@
+---
+bump: patch
+type: fix
+---
+
+Fix matching on backtrace paths containing spaces.
+
+When using `matchBacktracePaths`, when a backtrace line path contains a space, it will now match correctly against the whole path.
\ No newline at end of file
diff --git a/packages/javascript/src/__tests__/span.test.ts b/packages/javascript/src/__tests__/span.test.ts
index 6ce60a45..7adc6ab9 100644
--- a/packages/javascript/src/__tests__/span.test.ts
+++ b/packages/javascript/src/__tests__/span.test.ts
@@ -89,6 +89,35 @@ describe("Span", () => {
       })
     })
 
+    it("cleans Chrome-style backtraces with spaces in the path", () => {
+      const error = new Error("test error")
+      error.stack = [
+        "Error: test error",
+        "    at Foo (http://localhost:8080/assets with space/app/bundle.js:13:10)",
+        "    at Bar (http://localhost:8080/assets with space/app/bundle.js:17:10)",
+        "    at track (http://thirdparty.app/script.js:1:530)",
+        "    at http://localhost:8080/assets with space/app/bundle.js:21:10"
+      ].join("\n")
+
+      span.setError(error)
+      span.cleanBacktracePath(
+        [new RegExp("/assets with space/(app/.*)$")].map(toBacktraceMatcher)
+      )
+
+      const backtrace = span.serialize().error.backtrace
+      expect(backtrace).toEqual([
+        "Error: test error",
+        "    at Foo (app/bundle.js:13:10)",
+        "    at Bar (app/bundle.js:17:10)",
+        "    at track (http://thirdparty.app/script.js:1:530)",
+        "    at app/bundle.js:21:10"
+      ])
+
+      expect(span.serialize().environment).toMatchObject({
+        backtrace_paths_matched: "3"
+      })
+    })
+
     it("cleans Safari/FF-style backtraces", () => {
       const error = new Error("test error")
       error.stack = [
@@ -116,6 +145,33 @@ describe("Span", () => {
       })
     })
 
+    it("cleans Safari/FF-style backtraces with spaces in the path", () => {
+      const error = new Error("test error")
+      error.stack = [
+        "Foo@http://localhost:8080/assets with space/app/bundle.js:13:10",
+        "Bar@http://localhost:8080/assets with space/app/bundle.js:17:10",
+        "track@http://thirdparty.app/script.js:1:530",
+        "@http://localhost:8080/assets with space/app/bundle.js:21:10"
+      ].join("\n")
+
+      span.setError(error)
+      span.cleanBacktracePath(
+        [new RegExp("/assets with space/(app/.*)$")].map(toBacktraceMatcher)
+      )
+
+      const backtrace = span.serialize().error.backtrace
+      expect(backtrace).toEqual([
+        "Foo@app/bundle.js:13:10",
+        "Bar@app/bundle.js:17:10",
+        "track@http://thirdparty.app/script.js:1:530",
+        "@app/bundle.js:21:10"
+      ])
+
+      expect(span.serialize().environment).toMatchObject({
+        backtrace_paths_matched: "3"
+      })
+    })
+
     it("concatenates all match groups", () => {
       const error = new Error("test error")
       error.stack = [
diff --git a/packages/javascript/src/span.ts b/packages/javascript/src/span.ts
index a006dfb3..ddd4cd0f 100644
--- a/packages/javascript/src/span.ts
+++ b/packages/javascript/src/span.ts
@@ -186,15 +186,22 @@ function extractPath(backtraceLine: string): string | undefined {
   // A Chrome backtrace line always contains `at` near the beginning,
   // preceded by space characters and followed by one space.
   const IS_CHROME = /^\s*at\s/
+
   // In a Chrome backtrace line, the path (if it is available)
-  // is located, usually within parentheses, after the "@" towards the
-  // end of the line, along with the line number and column number,
-  // separated by colons. We check for those to reject clear non-paths.
-  const CHROME_PATH = /at(?:\s.*)?\s\(?(.*):\d*:\d*\)?$/i
+  // is located after the "at" towards the end of the line, along with
+  // the line number and column number, separated by colons.
+  // When the function name is available, the path is enclosed in
+  // parentheses. When there is no function name, the path comes
+  // immediately after the "at".
+  // We check for the line and column numbers to reject clear non-paths.
+  const CHROME_PATH_WITH_FUNCTION_NAME = /^\s*at(?:\s[^\(]+)?\s\((.*):\d+:\d+\)$/
+  const CHROME_PATH_WITHOUT_FUNCTION_NAME = /^\s*at\s(.*):\d+:\d+$/
 
   if (backtraceLine.match(IS_CHROME)) {
-    const match = backtraceLine.match(CHROME_PATH)
-    return match ? match[1] : undefined
+    return (
+      backtraceLine.match(CHROME_PATH_WITH_FUNCTION_NAME)?.[1] ??
+      backtraceLine.match(CHROME_PATH_WITHOUT_FUNCTION_NAME)?.[1]
+    )
   }
 
   // A Safari or Firefox backtrace line always contains `@` after the first
@@ -205,10 +212,9 @@ function extractPath(backtraceLine: string): string | undefined {
   // is located after the "@" at the towards end of the line, followed by
   // the line number and column number, separated by colons. We check for
   // those to reject clear non-paths.
-  const SAFARI_FF_PATH = /@\s?(.*):\d*:\d*$/i
+  const SAFARI_FF_PATH = /^.*@\s?(.*):\d+:\d+$/
 
   if (backtraceLine.match(IS_SAFARI_FF)) {
-    const match = backtraceLine.match(SAFARI_FF_PATH)
-    return match ? match[1] : undefined
+    return backtraceLine.match(SAFARI_FF_PATH)?.[1]
   }
 }